{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:09:17Z","timestamp":1790834957981,"version":"4.1.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100009837","name":"Canadian Food Inspection Agency","doi-asserted-by":"crossref","award":["OLF-F-1602"],"award-info":[{"award-number":["OLF-F-1602"]}],"id":[{"id":"10.13039\/100009837","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,12,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rapid identification of foods and food-processing environments contaminated with\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            is crucial for controlling human listeriosis, a leading cause of fatality related to foodborne illnesses. The\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            autolysin IspC anchors to the bacterial surface non-covalently via its C-terminal cell wall-binding domain (CWBD), which contains conserved Gly-Trp (GW) dipeptides. This study explored the binding property of CWBD\n            <jats:sub>IspC<\/jats:sub>\n            to design an affinity probe for detecting\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            . Immunogold electron microscopy analysis of a \u2206\n            <jats:italic>ispC<\/jats:italic>\n            mutant and its wild type (WT) revealed that exogenously added CWBD\n            <jats:sub>IspC<\/jats:sub>\n            bound to surface ligands that were evenly distributed and remained mostly unoccupied in the wild type. CWBD\n            <jats:sub>IspC<\/jats:sub>\n            detected, via recognizing the cell wall component lipoteichoic acid,\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            strains primarily belonging to the serotype 4 group, as well as\n            <jats:italic>Listeria innocua<\/jats:italic>\n            , and\n            <jats:italic>Listeria welshimeri<\/jats:italic>\n            but did not detect three other\n            <jats:italic>Listeria<\/jats:italic>\n            spp. (\n            <jats:italic>Listeria seeligeri<\/jats:italic>\n            ,\n            <jats:italic>Listeria ivanovii,<\/jats:italic>\n            and\n            <jats:italic>Listeria grayi<\/jats:italic>\n            ) nor any non-\n            <jats:italic>Listeria<\/jats:italic>\n            spp. tested. CWBD\n            <jats:sub>IspC<\/jats:sub>\n            , when tagged with the enhanced green fluorescent protein, was capable of detecting\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            by fluorescence microscopy, a colony lift filter assay, and a microtube binding assay. CWBD\n            <jats:sub>IspC<\/jats:sub>\n            , when covalently attached to magnetic beads, showed the ability to separate\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            cells with a maximum capture rate of 90 to nearly 100% from 1 \u00d7 10\n            <jats:sup>3<\/jats:sup>\n            to 1 \u00d7 10\n            <jats:sup>5<\/jats:sup>\n            target cells. This study demonstrated for the first time that the CWBD derived from a GW surface protein IspC is a novel antibody alternative for the rapid detection and isolation of\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            despite binding to two other\n            <jats:italic>Listeria<\/jats:italic>\n            species.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Human listeriosis is caused by consuming foods contaminated with the bacterial pathogen\n              <jats:italic>Listeria monocytogenes<\/jats:italic>\n              , leading to the development of a severe and life-threatening foodborne illness. Detection of\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              present in food and food processing environments is crucial for preventing\n              <jats:italic>Listeria<\/jats:italic>\n              infection. The\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              peptidoglycan hydrolase IspC anchors non-covalently to the bacterial surface through its C-terminal cell wall-binding domain (CWBD), CWBD\n              <jats:sub>IspC<\/jats:sub>\n              . This study explored the surface binding property of CWBD\n              <jats:sub>IspC<\/jats:sub>\n              to design, construct, characterize, and assess an affinity molecular probe for detecting\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              . CWBD\n              <jats:sub>IspC<\/jats:sub>\n              recognized a cell wall ligand lipoteichoic acid that remains evenly displayed and mostly unoccupied on the bacterial surface for interaction with the exogenously added CWBD\n              <jats:sub>IspC<\/jats:sub>\n              . CWBD\n              <jats:sub>IspC<\/jats:sub>\n              , when fused to the enhanced green fluorescent protein reporter or covalently conjugated onto magnetic beads, exhibited the functionality as an antibody alternative for rapid detection and efficient separation of the pathogen.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.05356-22","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T13:01:09Z","timestamp":1696510869000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Design of a novel affinity probe using the cell wall-binding domain of a\n            <i>Listeria monocytogenes<\/i>\n            autolysin for pathogen detection"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7351-6046","authenticated-orcid":false,"given":"Min","family":"Lin","sequence":"first","affiliation":[{"name":"Ottawa Laboratory (Fallowfield), Canadian Food Inspection Agency , Ottawa, Ontario, Canada"},{"name":"Department of Biochemistry Microbiology and Immunology, University of Ottawa , Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanhong","family":"Dan","sequence":"additional","affiliation":[{"name":"Ottawa Laboratory (Fallowfield), Canadian Food Inspection Agency , Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.011"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.05.002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028x-65.11.1811"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0740-0020(89)80025-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.11.3478-3481.1990"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.6.1924-1929.1992"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.7.2827-2832.1999"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2003.07.002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.10.6138-6146.2004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.11.3.446-451.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46305-0"},{"key":"e_1_3_2_13_2","first-page":"1152","article-title":"Production of monoclonal antibody against Listeria monocytogenes and its application to immunochromatography strip test","volume":"17","author":"Shim WB","year":"2007","unstructured":"Shim WB , Choi JG , Kim JY , Yang ZY , Lee KH , Kim MG , Ha SD , Kim KS , Kim KY , Kim CH , Ha KS , Eremin SA , Chung DH . 2007. Production of monoclonal antibody against Listeria monocytogenes and its application to immunochromatography strip test. J Microbiol Biotechnol 17:1152\u20131161.","journal-title":"J Microbiol Biotechnol"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2008.04140.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2014.07.049"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00774-16"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01908-16"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00035-22"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0966-842x(02)02342-9"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00122.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.60.080805.142256"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00039-06"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46977-0"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01375-06"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cws100"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/015172-0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4621825.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01652.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14472"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.2.712-718.2001"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4101774.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00983.x"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsb724"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02889.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2011.00263.x"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02402-06"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2009.0475"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/v10110626"},{"key":"e_1_3_2_39_2","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"2001","unstructured":"Sambrook J , Russell D . 2001. Molecular cloning: a laboratory manual. 3rd ed. Cold Spring Harbor Laboratory, New York.","edition":"3"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2020.106121"},{"key":"e_1_3_2_41_2","first-page":"241","article-title":"A pair of ligation-independent Escherichia coli expression vectors for rapid addition of a polyhistidine affinity tag to the N- or C-termini of recombinant proteins","volume":"20","author":"Dan H","year":"2009","unstructured":"Dan H , Balachandran A , Lin M . 2009. A pair of ligation-independent Escherichia coli expression vectors for rapid addition of a polyhistidine affinity tag to the N- or C-termini of recombinant proteins. J Biomol Tech 20:241\u2013248.","journal-title":"J Biomol Tech"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.12.218"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1976.9999"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055098"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00808-12"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1263\/jbb.104.55"},{"key":"e_1_3_2_47_2","first-page":"1235","volume-title":"Bergey\u2019s manual of systematic bacteriology","author":"Seeliger HPR","year":"1986","unstructured":"Seeliger HPR , Jones D . 1986. Genus Listeria, p 1235\u20131245. In Sneath PHA , NS Mair , ME Shrape (ed), Bergey\u2019s manual of systematic bacteriology. Williams Wilkins Co, Baltimore, Md."},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1097(90)90178-s"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.2007.01629.x"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-384730-0.00186-5"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.4060\/cc2400en"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2022.0079"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.8.4401-4409.2004"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.05356-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.05356-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T13:24:53Z","timestamp":1702387493000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.05356-22"}},"subtitle":[],"editor":[{"given":"Jasna","family":"Kovac","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,12,12]]},"references-count":52,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,12,12]]}},"alternative-id":["10.1128\/spectrum.05356-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.05356-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,12]]},"assertion":[{"value":"2022-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e05356-22"}}