{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:32:43Z","timestamp":1760783563770},"reference-count":0,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1978,1,1]],"date-time":"1978-01-01T00:00:00Z","timestamp":252460800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1978,1]]},"abstract":"<jats:p>\n            Conditions are described for the formation of protoplasts from\n            <jats:italic>Streptomyces parvulus<\/jats:italic>\n            that are able to synthesize actinomycin D de novo. Antibiotic synthesis by protoplasts, in contrast to that by mycelium, was sensitive to inhibition by actinomycin D and to a decrease in sucrose concentration. On the other hand, synthesis by mycelium was much more sensitive to inhibition by amino acid analogs (\n            <jats:sc>d<\/jats:sc>\n            -valine,\n            <jats:italic>cis<\/jats:italic>\n            -3-methylproline, and \u03b1-methyl-\n            <jats:sc>dl<\/jats:sc>\n            -tryptophan). In addition, the uptake of amino acids (\n            <jats:sc>l<\/jats:sc>\n            -methionine, sarcosine, and\n            <jats:sc>l<\/jats:sc>\n            - and\n            <jats:sc>d<\/jats:sc>\n            -valine) by protoplasts was significantly lower than that by mycelium. The advantages and limitations of using protoplasts for studying in vivo actinomycin synthesis are discussed.\n          <\/jats:p>","DOI":"10.1128\/aac.13.1.104","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T23:57:02Z","timestamp":1340841422000},"page":"104-114","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Actinomycin Biosynthesis by Protoplasts Derived from\n            <i>Streptomyces parvulus<\/i>"],"prefix":"10.1128","volume":"13","author":[{"given":"Michael J. M.","family":"Hitchcock","sequence":"first","affiliation":[{"name":"Department of Microbiology, Georgetown University Schools of Medicine and Dentistry, Washington, D.C. 20007"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Katz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Georgetown University Schools of Medicine and Dentistry, Washington, D.C. 20007"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.13.1.104","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.13.1.104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:15:26Z","timestamp":1645485326000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.13.1.104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1978,1]]}},"alternative-id":["10.1128\/AAC.13.1.104"],"URL":"https:\/\/doi.org\/10.1128\/aac.13.1.104","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,1]]},"assertion":[{"value":"1978-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}