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To evaluate the impact of methicillin resistance (\n            <jats:italic>mec<\/jats:italic>\n            ) determinants on bacterial adhesion mediated by fibrinogen or fibronectin adhesins, we compared the in vitro attachment of two genetically distinct susceptible strains (NCTC8325 and Newman) to protein-coated surfaces with that of isogenic methicillin-resistant derivatives. All strains containing an intact\n            <jats:italic>mec<\/jats:italic>\n            element in their chromosomes were found to be defective in adhesion to fibrinogen and fibronectin immobilized on polymethylmethacrylate coverslips, regardless of the presence or absence of additional mutations in the\n            <jats:italic>femA<\/jats:italic>\n            ,\n            <jats:italic>femB<\/jats:italic>\n            , or\n            <jats:italic>femC<\/jats:italic>\n            gene, known to decrease expression of methicillin resistance in\n            <jats:italic>S. aureus<\/jats:italic>\n            . Western ligand affinity blotting or immunoblotting of cell wall-associated adhesins revealed similar contents of fibrinogen- or fibronectin-binding proteins in methicillin-resistant strains compared to those of their methicillin-susceptible counterparts. In contrast to methicillin-resistant strains carrying a\n            <jats:italic>mec<\/jats:italic>\n            element in their genomes, methicillin-resistant strains constructed in vitro, by introducing the\n            <jats:italic>mecA<\/jats:italic>\n            gene on a plasmid, retained their adhesion phenotypes. In conclusion, the chromosomal insertion of the\n            <jats:italic>mec<\/jats:italic>\n            element into genetically defined strains of\n            <jats:italic>S. aureus<\/jats:italic>\n            impairs the in vitro functional activities of fibrinogen or fibronectin adhesins without altering their production. This effect is unrelated to the activity of the\n            <jats:italic>mecA<\/jats:italic>\n            gene.\n          <\/jats:p>","DOI":"10.1128\/aac.42.3.564","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:30:35Z","timestamp":1539045035000},"page":"564-570","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Introduction of the\n            <i>mec<\/i>\n            Element (Methicillin Resistance) into\n            <i>Staphylococcus aureus<\/i>\n            Alters In Vitro Functional Activities of Fibrinogen and Fibronectin Adhesins"],"prefix":"10.1128","volume":"42","author":[{"given":"Pierre E.","family":"Vaudaux","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Division of Infectious Diseases, University Hospital, Geneva,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenza","family":"Monzillo","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Infectious Diseases, University Hospital, Geneva,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Francois","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Infectious Diseases, University Hospital, Geneva,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel P.","family":"Lew","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Infectious Diseases, University Hospital, Geneva,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim J.","family":"Foster","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Microbiology Department, Moyne Institute, Trinity College, Dublin 2, Ireland2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Berger-Ba\u0308chi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Institute of Medical Microbiology, University of Zu\u0308rich, Zu\u0308rich,3 Switzerland, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.165.2.373-378.1986"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.154.1.479-487.1983"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1159\/000239498","article-title":"Update on methicillin resistance mechanisms in staphylococci.","volume":"42","author":"Berger-B\u00e4chi B.","year":"1996","unstructured":"Berger-B\u00e4chi B. 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