{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T01:35:07Z","timestamp":1768095307082,"version":"3.49.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,3,15]],"date-time":"2012-03-15T00:00:00Z","timestamp":1331769600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The polyhydroxyalkanoate (PHA) granule-associated proteins (PGAPs) are important for PHA synthesis and granule formation, but currently little is known about the haloarchaeal PGAPs. This study focused on the identification and functional analysis of the PGAPs in the haloarchaeon\n            <jats:named-content content-type=\"genus-species\">Haloferax mediterranei<\/jats:named-content>\n            . These PGAPs were visualized with two-dimensional gel electrophoresis (2-DE) and identified by matrix-assisted laser desorption ionization\u2013tandem time of flight mass spectrometry (MALDI-TOF\/TOF MS). The most abundant protein on the granules was identified as a hypothetical protein, designated PhaP. A genome-wide analysis revealed that the\n            <jats:italic>phaP<\/jats:italic>\n            gene is located upstream of the previously identified\n            <jats:italic>phaEC<\/jats:italic>\n            genes. Through an integrative approach of gene knockout\/complementation and fermentation analyses, we demonstrated that this PhaP is involved in PHA accumulation. The \u0394\n            <jats:italic>phaP<\/jats:italic>\n            mutant was defective in both PHA biosynthesis and cell growth compared to the wild-type strain. Additionally, transmission electron microscopy results indicated that the number of PHA granules in the \u0394\n            <jats:italic>phaP<\/jats:italic>\n            mutant cells was significantly lower, and in most of the \u0394\n            <jats:italic>phaP<\/jats:italic>\n            cells only a single large granule was observed. These results demonstrated that the\n            <jats:named-content content-type=\"genus-species\">H. mediterranei<\/jats:named-content>\n            PhaP was the predominant structure protein (phasin) on the PHA granules involved in PHA accumulation and granule formation. In addition, BLASTp and phylogenetic results indicate that this type of PhaP is exclusively conserved in haloarchaea, implying that it is a representative of the haloarchaeal type PHA phasin.\n          <\/jats:p>","DOI":"10.1128\/aem.07114-11","type":"journal-article","created":{"date-parts":[[2012,1,14]],"date-time":"2012-01-14T20:08:29Z","timestamp":1326571709000},"page":"1946-1952","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":107,"title":["Identification of the Haloarchaeal Phasin (PhaP) That Functions in Polyhydroxyalkanoate Accumulation and Granule Formation in Haloferax mediterranei"],"prefix":"10.1128","volume":"78","author":[{"given":"Shuangfeng","family":"Cai","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}]},{"given":"Lei","family":"Cai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}]},{"given":"Hailong","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}]},{"given":"Xiaoqing","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}]},{"given":"Jing","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}]},{"given":"Jian","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}]},{"given":"Hua","family":"Xiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.54.4.450-472.1990"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1002\/pmic.200300525","article-title":"Improvement of the two-dimensional gel electrophoresis analysis for the proteome study of Halobacterium salinarum","volume":"3","author":"Cho CW","year":"2003","unstructured":"ChoCW. 2003. Improvement of the two-dimensional gel electrophoresis analysis for the proteome study of Halobacterium salinarum. Proteomics 3:2325\u20132329.","journal-title":"Proteomics"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1139\/m89-022"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05469-11"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01668-07"},{"key":"e_1_3_2_7_2","first-page":"1305","article-title":"Identification of the phaB genes and analysis of the PHBV precursor supplying pathway in Haloferax mediterranei","volume":"50","author":"Feng B","year":"2010","unstructured":"FengB. 2010. Identification of the phaB genes and analysis of the PHBV precursor supplying pathway in Haloferax mediterranei. Wei Sheng Wu Xue Bao 50:1305\u20131312.","journal-title":"Wei Sheng Wu Xue Bao"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.51.1.214-216.1986"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07450.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00850a047"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01117-10"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00938-09"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00953-07"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.8.2466-2475.2004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01723-08"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1002\/pmic.200400950","article-title":"Improvement of two-dimensional gel electrophoresis proteome maps of the haloarchaeon Haloferax volcanii","volume":"5","author":"Karadzic IM","year":"2005","unstructured":"KaradzicIM Maupin-FurlowJA. 2005. Improvement of two-dimensional gel electrophoresis proteome maps of the haloarchaeon Haloferax volcanii. Proteomics 5:354\u2013359.","journal-title":"Proteomics"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.ab.2006.01.017","article-title":"Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins","volume":"351","author":"Kirkland PA","year":"2006","unstructured":"KirklandPA BusbyJ StevensSJr Maupin-FurlowJA. 2006. Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins. Anal. Biochem. 351:254\u2013259.","journal-title":"Anal. Biochem"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.5.1987"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.14.5478"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19960105)49:1<1::AID-BIT1>3.0.CO;2-P"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2611-6"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2011.05.003"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00134-08"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.2.585-592.1999"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01486-07"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.051508-0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07869.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.9.2513-2523.1995"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26970-0"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/bm049401n"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.3.858-868.1999"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1186\/1471-2180-10-254","article-title":"Influence of growth stage on activities of polyhydroxyalkanoate (PHA) polymerase and PHA depolymerase in Pseudomonas putida U","volume":"10","author":"Ren Q","year":"2010","unstructured":"RenQ de RooG WitholtB ZinnM Th\u00f6ny-MeyerL. 2010. Influence of growth stage on activities of polyhydroxyalkanoate (PHA) polymerase and PHA depolymerase in Pseudomonas putida U. BMC Microbiol. 10:254.","journal-title":"BMC Microbiol"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.12.7530-7538.2004"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/bm8001655"},{"key":"e_1_3_2_35_2","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"2001","unstructured":"SambrookJ RussellDW. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.","edition":"3"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2006.01196.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac950914h"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1139\/m95-175"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(98)01194-9"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.11.3814-3824.2005"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01190-07"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.9.2425-2435.1995"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.7.2394-2397.2001"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.07114-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.07114-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T01:35:54Z","timestamp":1645580154000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.07114-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,15]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,3,15]]}},"alternative-id":["10.1128\/AEM.07114-11"],"URL":"https:\/\/doi.org\/10.1128\/aem.07114-11","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,15]]},"assertion":[{"value":"2011-10-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-02-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}