{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T23:25:21Z","timestamp":1769383521180,"version":"3.49.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[1998,10,1]],"date-time":"1998-10-01T00:00:00Z","timestamp":907200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Strains of\n            <jats:italic>Pseudomonas syringae<\/jats:italic>\n            pv. syringae were isolated from healthy and diseased stone fruit tissues sampled from 43 orchard sites in California in 1995 and 1996. These strains, together with\n            <jats:italic>P. syringae<\/jats:italic>\n            strains from other hosts and pathovars, were tested for pathogenicity and the presence of the\n            <jats:italic>syrB<\/jats:italic>\n            and\n            <jats:italic>syrC<\/jats:italic>\n            genes and were genetically characterized by using enterobacterial repetitive intergenic consensus (ERIC) primers and PCR. All 89 strains of\n            <jats:italic>P. syringae<\/jats:italic>\n            pv. syringae tested were moderately to highly pathogenic on Lovell peach seedlings regardless of the host of origin, while strains of other pathovars exhibited low or no pathogenicity. The 19 strains of\n            <jats:italic>P. syringae<\/jats:italic>\n            pv. syringae examined by restriction fragment length polymorphism analysis contained the\n            <jats:italic>syrB<\/jats:italic>\n            and\n            <jats:italic>syrC<\/jats:italic>\n            genes, whereas no hybridization occurred with 4 strains of other\n            <jats:italic>P. syringae<\/jats:italic>\n            pathovars. The\n            <jats:italic>P. syringae<\/jats:italic>\n            pv. syringae strains from stone fruit, except for a strain from New Zealand, generated ERIC genomic fingerprints which shared four fragments of similar mobility. Of the\n            <jats:italic>P. syringae<\/jats:italic>\n            pv. syringae strains tested from other hosts, only strains from rose, kiwi, and pear generated genomic fingerprints that had the same four fragments as the stone fruit strains. Analysis of the ERIC fingerprints from\n            <jats:italic>P. syringae<\/jats:italic>\n            pv. syringae strains showed that the strains isolated from stone fruits formed a distinct cluster separate from most of the strains isolated from other hosts. These results provide evidence of host specialization within the diverse pathovar\n            <jats:italic>P. syringae<\/jats:italic>\n            pv. syringae.\n          <\/jats:p>","DOI":"10.1128\/aem.64.10.3818-3823.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T18:54:36Z","timestamp":1576781676000},"page":"3818-3823","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":77,"title":["Genetic Characterization of\n            <i>Pseudomonas syringae<\/i>\n            pv. syringae Strains from Stone Fruits in California"],"prefix":"10.1128","volume":"64","author":[{"given":"E. L.","family":"Little","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Plant Pathology, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M.","family":"Bostock","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Plant Pathology, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. C.","family":"Kirkpatrick","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Plant Pathology, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Bradbury\nJ. F. Pseudomonas syringae pv. syringae Guide to Plant Pathogenic Bacteria.\n1986\n175\n177\nCAB International Mycological Institute\nKew England"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1094\/PD-73-0419"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.6.1743-1749.1990"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.7.2180-2187.1992"},{"key":"e_1_3_2_6_2","first-page":"1949","article-title":"Genetic diversity and relationships of two pathovars of Pseudomonas syringae.","volume":"134","author":"Denny T. P.","year":"1988","unstructured":"Denny T. P. Gilmour M. N. Selander R. K. Genetic diversity and relationships of two pathovars of Pseudomonas syringae. J. Gen. Microbiol. 134 1988 1949 1960","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_7_2","first-page":"95","article-title":"A biocide produced by pathogenic isolates of Pseudomonas syringae and its possible role in the bacterial canker disease of peach trees.","volume":"58","author":"DeVay J. E.","year":"1968","unstructured":"DeVay J. E. Lukezic F. L. Sinden S. L. English H. Coplin D. L. A biocide produced by pathogenic isolates of Pseudomonas syringae and its possible role in the bacterial canker disease of peach trees. Phytopathology 58 1968 95 101","journal-title":"Phytopathology"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-64-1330"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-79-518"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-74-241"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-67-475"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1992.tb05005.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.py.20.090182.001315"},{"key":"e_1_3_2_14_2","first-page":"301","article-title":"Two simple media for the demonstration of pyocyanin and fluorescein.","volume":"44","author":"King E. O.","year":"1954","unstructured":"King E. O. Ward M. K. Raney D. E. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44 1954 301 307","journal-title":"J. Lab. Clin. Med."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-63-1099"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-69-1122"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.12.4180-4188.1993"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1966.tb03499.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","unstructured":"Little\nE. L.\nGilbertson\nR. L.\nPhenotypic and genotypic characters support placement of Pseudomonas syringae strains from tomato celery and cauliflower into distinct pathovars\nPseudomonas syringae pathovars and related pathogens.\nRudolph\nK.\nBurr\nT. J.\nMansfield\nJ. W.\nStead\nD.\nVivian\nA.\nvon Kietzell\nJ.\n1997\n542\n547\nKluwer Academic Publishers\nDordrecht The Netherlands","DOI":"10.1007\/978-94-011-5472-7_99"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.7.2286-2295.1994"},{"key":"e_1_3_2_21_2","unstructured":"Ogawa\nJ. M.\nEnglish\nH.\nDiseases of temperate zone tree fruit and nut crops. Publication 3345.\n1991\nUniversity of California Division of Agriculture and Natural Resources\nOakland"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1094\/PD-80-0868"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-61-443"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-7-0078"},{"key":"e_1_3_2_25_2","first-page":"5","article-title":"Weeds in orchards as potential source of inoculum for bacterial canker of stone fruit.","volume":"18","author":"Roos I. M. M.","year":"1986","unstructured":"Roos I. M. M. Hattingh M. J. Weeds in orchards as potential source of inoculum for bacterial canker of stone fruit. Phytophylactica 18 1986 5 6","journal-title":"Phytophylactica"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-77-900"},{"key":"e_1_3_2_27_2","first-page":"75","article-title":"Bacterial brown spot disease of bush bean (Phaseolus vulgaris L.) in Germany, incited by Pseudomonas syringae van Hall s. s. pathovar phaseoli.","volume":"86","author":"Rudolph K.","year":"1979","unstructured":"Rudolph K. Bacterial brown spot disease of bush bean (Phaseolus vulgaris L.) in Germany, incited by Pseudomonas syringae van Hall s. s. pathovar phaseoli. Z. Pflanzenkr. Pflanzenschutz 86 1979 75 85","journal-title":"Z. Pflanzenkr. Pflanzenschutz"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-62-678"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-79-903"},{"key":"e_1_3_2_30_2","unstructured":"Seem\u00fcller\nE.\nArnold\nM.\nPathogenicity syringomycin production and other characteristics of pseudomonad strains isolated from deciduous fruit trees\nProceedings of the 4th International Conference on Plant Pathogenic Bacteria\n1978\n703\n710"},{"key":"e_1_3_2_31_2","unstructured":"Sokal\nR. R.\nSneath\nP. H. A.\nPrinciples of numerical taxonomy\n1963\n169\n210\nW. H. Freeman & Co.\nSan Francisco Calif"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.12.4421-4431.1994"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0434.1997.tb00411.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7348.1991.tb05629.x"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.10.3818-3823.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.10.3818-3823.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T05:34:09Z","timestamp":1645594449000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.64.10.3818-3823.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,10]]},"references-count":34,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1998,10]]}},"alternative-id":["10.1128\/AEM.64.10.3818-3823.1998"],"URL":"https:\/\/doi.org\/10.1128\/aem.64.10.3818-3823.1998","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,10]]},"assertion":[{"value":"1998-01-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-07-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}