{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:33:48Z","timestamp":1762868028167},"reference-count":29,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1998,6,1]],"date-time":"1998-06-01T00:00:00Z","timestamp":896659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Active biological containment systems consist of two components, a killing element designed to induce cell death and a control element which modulates the expression of the killing function. We constructed a mini-Tn\n            <jats:italic>5<\/jats:italic>\n            transposon bearing a fusion of the P\n            <jats:sub>\n              <jats:italic>lac<\/jats:italic>\n            <\/jats:sub>\n            promoter to the\n            <jats:italic>gef<\/jats:italic>\n            killing gene and a fusion of the Pm promoter to the\n            <jats:italic>lacI<\/jats:italic>\n            gene plus the positive regulator of the Pm promoter, the\n            <jats:italic>xylS<\/jats:italic>\n            gene. This mini-Tn\n            <jats:italic>5<\/jats:italic>\n            transposon was transferred to the chromosome of\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            CMC4, and in culture this strain survived in the presence of 3-methylbenzoate (an XylS effector) and committed suicide in the absence of this aromatic compound. The rate of killing escape was on the order of 10\n            <jats:sup>\u22128<\/jats:sup>\n            per cell and per generation. This contained strain and an uncontained control strain were used in outdoor tests performed in the spring-summer and autumn-winter periods to determine their survival in planted and unplanted soils with and without 3-methylbenzoate. In unplanted soils the numbers of both the contained strain and the uncontained strain per gram of soil tended to decrease, but the numbers of the contained strain decreased faster in soils without 3-methylbenzoate. The decrease in the number of CFU per gram of soil was faster in the spring-summer period than in the autumn-winter period. In planted soils survival in the rhizosphere and survival in bulk soil were studied. In the rhizosphere the uncontained control strain tended to become established at levels on the order of 10\n            <jats:sup>5<\/jats:sup>\n            to 10\n            <jats:sup>6<\/jats:sup>\n            CFU\/g of soil regardless of the presence of 3-methylbenzoate. In the bulk soil the numbers of bacterial cells were 2 to 3 orders of magnitude lower. In planted soils the contained strain tended to disappear, but this tendency was more pronounced in the absence of 3-methylbenzoate and occurred faster in the summer assay than in the winter assay. We found no evidence of dispersal of the test strains outside the experimental plots.\n          <\/jats:p>","DOI":"10.1128\/aem.64.6.2072-2078.1998","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T19:36:11Z","timestamp":1576784171000},"page":"2072-2078","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Construction of an Efficient Biologically Contained\n            <i>Pseudomonas putida<\/i>\n            Strain and Its Survival in Outdoor Assays"],"prefix":"10.1128","volume":"64","author":[{"given":"La\u0301zaro","family":"Molina","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->GX-Biosystems Espan\u0303a, Granada,1 and"}]},{"given":"Cayo","family":"Ramos","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Biochemistry and Molecular and Cellular Biology of Plants, Estacio\u0301n Experimental del Zaid\u0131\u0301n, Consejo Superior de Investigaciones Cient\u0131\u0301ficas, 18008 Granada,2 Spain, and"},{"name":"<!--label omitted: 3-->Department of Microbiology, Technical University of Denmark, Lyngby, Denmark3"}]},{"given":"Mari\u0301a-Carmen","family":"Ronchel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Biochemistry and Molecular and Cellular Biology of Plants, Estacio\u0301n Experimental del Zaid\u0131\u0301n, Consejo Superior de Investigaciones Cient\u0131\u0301ficas, 18008 Granada,2 Spain, and"}]},{"given":"S\u00f8ren","family":"Molin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Microbiology, Technical University of Denmark, Lyngby, Denmark3"}]},{"given":"Juan L.","family":"Ramos","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Biochemistry and Molecular and Cellular Biology of Plants, Estacio\u0301n Experimental del Zaid\u0131\u0301n, Consejo Superior de Investigaciones Cient\u0131\u0301ficas, 18008 Granada,2 Spain, and"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.12.6782-6790.1989"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260150402"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0958-1669(94)90028-0"},{"key":"e_1_3_2_5_2","first-page":"141","article-title":"Expression and secretion of foreign genes in yeast","author":"Carter B. 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Biotechnology 28 1995 65 102","journal-title":"Biotechnology"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.11.3713-3717.1993"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.1.85-92.1991"},{"key":"e_1_3_2_15_2","first-page":"59","article-title":"The purification of eukaryotic polypeptides expressed in Escherichia coli","author":"Marston F. A. O.","year":"1987","unstructured":"Marston F. A. O. The purification of eukaryotic polypeptides expressed in Escherichia coli DNA cloning Glover D. M. III 1987 59 88 IRL Press Oxford, United Kingdom","journal-title":"DNA cloning"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.47.100193.001035"},{"key":"e_1_3_2_17_2","unstructured":"Ramos C. L. Molina S. Molin and J. L. Ramos. Colonization of the rhizosphere of corn ( Zea mays ) by a bioluminescent Pseudomonas putida strain and its competitiveness advantages on soil isolates. Submitted for publication."},{"key":"e_1_3_2_18_2","first-page":"1349","article-title":"The behavior of bacteria designed for biodegradation.","volume":"12","author":"Ramos J. L.","year":"1994","unstructured":"Ramos J. L. D\u0131\u0301az E. Dowling D. de Lorenzo V. Molin S. O\u2019Gara F. Ramos C. Timmis K. N. The behavior of bacteria designed for biodegradation. Bio\/Technology 12 1994 1349 1356","journal-title":"Bio\/Technology"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.1.260-266.1991"},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","unstructured":"Ramos\nJ. L.\nHa\u0131\u0308dour\nA.\nDelgado\nA.\nDuque\nE.\nFandila\nM.-D.\nGil\nM.\nPi\u00f1ar\nG.\nPotential of toluene-degrading systems for the construction of hybrid pathways for nitrotoluene metabolism\nBiodegradation of nitroaromatic compounds.\nSpain\nJ. C.\n1995\n53\n68\nPlenum Press\nNew York N.Y","DOI":"10.1007\/978-1-4757-9447-2_4"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0396-320"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.51.1.341"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.22.8467"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.15.4635-4641.1994"},{"key":"e_1_3_2_25_2","unstructured":"Rodr\u0131\u0301guez-Herva J. J. D. Reniero M. A. Ramos-D\u0131\u0301az L. Molina C. Ramos E. Galli and J. L. Ramos. LPS and OprL mutants of Pseudomonas putida exhibit increased sensitivity to drugs and limited capacity to colonize soils. Submitted for publication."},{"key":"e_1_3_2_26_2","unstructured":"Ronchel\nM. C.\nConstrucci\u00f3n de cepas de Pseudomonas putida portadoras de sistemas condicionales de contenci\u00f3n biol\u00f3gica para la eliminaci\u00f3n de contaminantes ambientales. Ph.D. thesis.\n1997\nUniversity of Granada\nGranada Spain"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.8.2990-2994.1995"},{"key":"e_1_3_2_28_2","unstructured":"Sambrook\nJ.\nFritsch\nE. F.\nManiatis\nT.\nMolecular cloning: a laboratory manual\n2nd ed.\n1989\nCold Spring Harbor Laboratory\nCold Spring Harbor N.Y"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00340213"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-9-1947"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.6.2072-2078.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.64.6.2072-2078.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T06:09:32Z","timestamp":1645596572000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.64.6.2072-2078.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,6]]},"references-count":29,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1998,6]]}},"alternative-id":["10.1128\/AEM.64.6.2072-2078.1998"],"URL":"https:\/\/doi.org\/10.1128\/aem.64.6.2072-2078.1998","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,6]]},"assertion":[{"value":"1997-11-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-03-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}