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To analyze where the immunogenic parts were localized, seven highly purified truncated fusion proteins constituting different regions of the protein were produced (<jats:italic>Chlamydia pneumoniae<\/jats:italic>-Omp2<jats:sub>aa23-aa93<\/jats:sub>,<jats:italic>Chlamydia psittaci<\/jats:italic>-Omp2<jats:sub>aa23-aa94<\/jats:sub>, and<jats:italic>Chlamydia trachomatis<\/jats:italic>-Omp2<jats:sub>aa23-aa84, aa87-aa547, aa23-aa182, aa167-aa434, aa420-aa547<\/jats:sub>). By an enzyme-linked immunosorbent assay with serologically defined patient sera, Omp2 was found to be a major immunogen of both<jats:italic>C. pneumoniae<\/jats:italic>and<jats:italic>C. trachomatis<\/jats:italic>infections (<jats:italic>P<\/jats:italic>\u226a 0.0001). The humoral immune responses were not confined to any particular region of the Omp2 protein, and no species-specific anti-Omp2 immunoglobulins were detected.<\/jats:p>","DOI":"10.1128\/cdli.5.3.313-318.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T19:20:25Z","timestamp":1577820025000},"page":"313-318","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Analysis of the Humoral Immune Response to<i>Chlamydia<\/i>Outer Membrane Protein 2"],"prefix":"10.1128","volume":"5","author":[{"given":"Per","family":"Mygind","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus, Denmark,1 and"}]},{"given":"Gunna","family":"Christiansen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus, Denmark,1 and"}]},{"given":"Kenneth","family":"Persson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Virology, The University Hospital, Malmo\u0308, Sweden2"}]},{"given":"Svend","family":"Birkelund","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus, Denmark,1 and"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830230529"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.6.1261-1264.1990"},{"key":"e_1_3_2_4_2","unstructured":"Christiansen G. \u00d8stergaard L. 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