{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T21:54:40Z","timestamp":1757541280186},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1984,2,1]],"date-time":"1984-02-01T00:00:00Z","timestamp":444441600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1984,2]]},"abstract":"<jats:p>Escherichia coli K-12 minicells were used to study the expression of plasmid pFK99 encoding for the production of K99 fimbriae. Plasmid pFK99 is composed of a 6.7-kilobase pair DNA fragment derived from the wild-type K99 plasmid and the vector pBR322. The cloned K99 DNA expressed seven polypeptides with apparent masses of 18.2, 19.0, 21.0, 21.5, 26.5, 33.5, and 76.0 kilodaltons (kd). The 18.2-kd polypeptide was identified as the K99 fimbrial subunit by reaction with specific anti-K99 antibodies. The fimbrial subunit and the 19.0-, 26.5-, 33.5-, and 76.0-kd polypeptides appeared to be synthesized in a precursor form which was ca. 2 kd larger than the mature polypeptide. The location of the structural genes encoding the seven polypeptides on the physical map of pFK99 was established by analyzing a set of deletion derivatives of pFK99. The gene encoding the fimbrial subunit was located at the promoter proximal end of the K99 operon. Only mutants with a deletion in the gene encoding the 33.5- or the 19.0-kd polypeptide or both showed a weak expression of the K99 antigen and a comparably weak agglutination of horse or sheep erythrocytes. None of the deletion mutants was able to adhere to calf intestinal epithelial cells.<\/jats:p>","DOI":"10.1128\/iai.43.2.508-514.1984","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T03:01:24Z","timestamp":1578020484000},"page":"508-514","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Organization and expression of genes involved in the biosynthesis of K99 fimbriae"],"prefix":"10.1128","volume":"43","author":[{"given":"F K","family":"de Graaf","sequence":"first","affiliation":[]},{"given":"B E","family":"Krenn","sequence":"additional","affiliation":[]},{"given":"P","family":"Klaasen","sequence":"additional","affiliation":[]}],"member":"235","container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.43.2.508-514.1984","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.43.2.508-514.1984","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T22:13:27Z","timestamp":1646432007000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.43.2.508-514.1984"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1984,2]]}},"alternative-id":["10.1128\/iai.43.2.508-514.1984"],"URL":"https:\/\/doi.org\/10.1128\/iai.43.2.508-514.1984","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,2]]},"assertion":[{"value":"1984-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}