{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T11:59:22Z","timestamp":1774785562225,"version":"3.50.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[1997,12,1]],"date-time":"1997-12-01T00:00:00Z","timestamp":880934400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1997,12]]},"abstract":"<jats:p>Infection of epithelial cells by two biovars of Chlamydia trachomatis results in the tyrosine phosphorylation of several host proteins. The most prominent change in host protein tyrosine phosphorylation involves a complex of proteins with molecular masses of 75 to 85 kDa (pp75\/85) and 100 kDa (pp100). The C. trachomatis-induced tyrosine phosphorylation of pp75\/85 and pp100 is observed in several cell lines, including epithelial cells, fibroblasts, and macrophages. Subcellular fractionation and detergent solubility properties of pp75\/85 are consistent with its association with the cytoskeleton. Phosphoamino acid analysis demonstrates that the pp75\/85 complex is phosphorylated on both tyrosine and serine residues. Immunofluorescence studies of chlamydia-infected cells by using fluorescein isothiocyanate-phalloidin and antibodies to phosphotyrosine and cortactin demonstrate that tyrosine-phosphorylated proteins, as well as cortactin, are localized to the chlamydial vacuole and that this process is facilitated by actin.<\/jats:p>","DOI":"10.1128\/iai.65.12.5301-5308.1997","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T19:11:34Z","timestamp":1578337894000},"page":"5301-5308","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["Infection with Chlamydia trachomatis alters the tyrosine phosphorylation and\/or localization of several host cell proteins including cortactin"],"prefix":"10.1128","volume":"65","author":[{"given":"F S","family":"Fawaz","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143-0654, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"van Ooij","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143-0654, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Homola","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143-0654, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S C","family":"Mutka","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143-0654, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J N","family":"Engel","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco 94143-0654, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.65.12.5301-5308.1997","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.65.12.5301-5308.1997","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:59:33Z","timestamp":1646470773000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.65.12.5301-5308.1997"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1997,12]]}},"alternative-id":["10.1128\/iai.65.12.5301-5308.1997"],"URL":"https:\/\/doi.org\/10.1128\/iai.65.12.5301-5308.1997","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,12]]},"assertion":[{"value":"1997-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}