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Associated with this rise in\n p<\/jats:italic>\n H is a reduced cell permeability to certain precursors of ribonucleic acid (RNA), deoxyribonucleic acid or protein. Uptake of adenine, alanine, and leucine was optimal at\n p<\/jats:italic>\n H 5.6 to 6.0, but sporulation was inhibited when the sporulation medium was buffered below\n p<\/jats:italic>\n H 7.0. Cellular impermeability can be largely overcome by adjusting the acetate sporulation medium to\n p<\/jats:italic>\n H 6.0 for optimal uptake of\n 14<\/jats:sup>\n C-adenine during short pulses without any apparent effect on sporulation. Sporulating cells pulse-labeled 20 min at\n p<\/jats:italic>\n H 6.0 incorporated 40 times more\n 14<\/jats:sup>\n C-adenine into RNA than sporulating cells pulse-labeled at\n p<\/jats:italic>\n H 8.0. This increased incorporation can be attributed to a 100-fold increase in labeled adenosine triphosphate in cells pulse-labeled at\n p<\/jats:italic>\n H 6.0 where maximum uptake occurs.\n <\/jats:p>","DOI":"10.1128\/jb.112.1.519-526.1972","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T14:38:43Z","timestamp":1578062323000},"page":"519-526","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Effect of\n p<\/i>\n H on Adenine and Amino Acid Uptake During Sporulation in\n Saccharomyces cerevisiae<\/i>"],"prefix":"10.1128","volume":"112","author":[{"given":"Dallice","family":"Mills","sequence":"first","affiliation":[{"name":"Department of Genetics SJ-10, University of Washington, Seattle, Washington 98105"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/S0021-9258(19)67936-6","article-title":"The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid","volume":"235","author":"Ames B. 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