{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T18:52:16Z","timestamp":1775674336946,"version":"3.50.1"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1974,5,1]],"date-time":"1974-05-01T00:00:00Z","timestamp":136598400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1974,5]]},"abstract":"<jats:p>\n            The limiting sizes of molecules that can permeate the intact cell wall and protoplast membrane of\n            <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n            were determined from the inflection points in a triphasic pattern of passive equilibrium uptake values obtained with a series of inert probing molecules varying in molecular size. In the phase identified with the yeast protoplast, the uptake-exclusion threshold corresponded to a monodisperse ethylene glycol of molecular weight = 110 and Einstein-Stokes hydrodynamic radius (\n            <jats:italic>\n              r\n              <jats:sub>ES<\/jats:sub>\n            <\/jats:italic>\n            ) = 0.42 nm. In the cell wall phase, the threshold corresponded to a polydisperse polyethylene glycol of number-average molecular weight (\n            <jats:italic>\n              \u00afM\n              <jats:sub>n<\/jats:sub>\n            <\/jats:italic>\n            ) = 620 and average radius (\n            <jats:italic>\n              r\n              <jats:sub>ES<\/jats:sub>\n            <\/jats:italic>\n            ) = 0.81 nm. The third phase corresponded to complete exclusion of larger molecules. The assessment of cell wall porosity was confirmed by use of a second method involving analytical gel chromatographic analyses of the molecular weight distribution for a single polydisperse polyglycol before and after uptake by the cells, which indicated a quasi-monodisperse threshold for the cell wall of\n            <jats:italic>\n              M\n              <jats:sub>n<\/jats:sub>\n            <\/jats:italic>\n            = 760 and\n            <jats:italic>\n              r\n              <jats:sub>ES<\/jats:sub>\n            <\/jats:italic>\n            = 0.89 nm. The results were reconciled with two situations in which much larger protein molecules previously have been reported able to penetrate the yeast cell wall.\n          <\/jats:p>","DOI":"10.1128\/jb.118.2.534-540.1974","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T15:00:48Z","timestamp":1578063648000},"page":"534-540","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":139,"title":["Porosity of the Yeast Cell Wall and Membrane"],"prefix":"10.1128","volume":"118","author":[{"given":"Rene","family":"Scherrer","sequence":"first","affiliation":[{"name":"Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise","family":"Louden","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipp","family":"Gerhardt","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1128\/jb.112.3.1346-1352.1972","article-title":"Location 6f acid phosphatase and ,8-fructofuranosidase within yeast cell envelopes","volume":"112","author":"Arnold W. 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