{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T06:42:02Z","timestamp":1759992122101},"reference-count":26,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1974,6,1]],"date-time":"1974-06-01T00:00:00Z","timestamp":139276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1974,6]]},"abstract":"<jats:p>Covalently closed extrachromosomal deoxyribonucleic acid (DNA) was isolated from alpha-hemolytic wild-type strains of<jats:italic>Escherichia coli<\/jats:italic>. Most strains examined were able to transfer the hemolytic property with varying frequencies to nonhemolytic recipient strains. Out of eight naturally isolated alphahemolytic<jats:italic>E. coli<\/jats:italic>strains, four contained a set of three different supercoiled DNAs with sedimentation coefficients of 76<jats:italic>S<\/jats:italic>(plasmid A), 63<jats:italic>S<\/jats:italic>(plasmid B), and 55<jats:italic>S<\/jats:italic>(plasmid C). The sedimentation coefficients and the contour lengths of the isolated molecules correspond to molecular weights of 65 \u00d7 10<jats:sup>6<\/jats:sup>, 41 \u00d7 10<jats:sup>6<\/jats:sup>, and 32 \u00d7 10<jats:sup>6<\/jats:sup>. Three alpha-hemolytic wild-type strains carried only one plasmid with a molecular weight of 41 \u00d7 10<jats:sup>6<\/jats:sup>, and one strain harbored two plasmids with molecular weights of 41 \u00d7 10<jats:sup>6<\/jats:sup>and 32 \u00d7 10<jats:sup>6<\/jats:sup>. Alpha-hemolytic transconjugants were obtained by conjugation of<jats:italic>E. coli<\/jats:italic>K-12 with the hemolytic wild-type strains. A detailed examination revealed that plasmids with the same sizes as plasmids B and C of the wild-type strains can be transferred separately or together to the recipients. Both plasmids possess the hemolytic determinant and transfer properties. Plasmid A appears to be, at least in one wild-type strain, an additional transfer factor without a hemolytic determinant. In one case a hemolytic factor was isolated, after conjugation, that is larger in size than plasmid A and appears to be a recombinant of both plasmids B and C.<\/jats:p>","DOI":"10.1128\/jb.118.3.964-973.1974","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T15:01:16Z","timestamp":1578063676000},"page":"964-973","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Plasmids Controlling Synthesis of Hemolysin in<i>Escherichia coli<\/i>: Molecular Properties"],"prefix":"10.1128","volume":"118","author":[{"given":"Werner","family":"Goebel","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie und Molekularbiologie der Universit\u00e4t Hohenheim, Hohenheim, Germany, and Institut f\u00fcr Molekulare Genetik der Universit\u00e4t Heidelberg, Heidelberg, Germany"}]},{"given":"Brigitte","family":"Royer-Pokora","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie und Molekularbiologie der Universit\u00e4t Hohenheim, Hohenheim, Germany, and Institut f\u00fcr Molekulare Genetik der Universit\u00e4t Heidelberg, Heidelberg, Germany"}]},{"given":"Werner","family":"Lindenmaier","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie und Molekularbiologie der Universit\u00e4t Hohenheim, Hohenheim, Germany, and Institut f\u00fcr Molekulare Genetik der Universit\u00e4t Heidelberg, Heidelberg, Germany"}]},{"given":"Hermann","family":"Bujard","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie und Molekularbiologie der Universit\u00e4t Hohenheim, Hohenheim, Germany, and Institut f\u00fcr Molekulare Genetik der Universit\u00e4t Heidelberg, Heidelberg, Germany"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/206579a0","article-title":"Drug resistance and its transf'er in Salmonella tfphimurium","volume":"206","author":"Anderson E. 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