{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T01:32:44Z","timestamp":1773624764377,"version":"3.50.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1975,4,1]],"date-time":"1975-04-01T00:00:00Z","timestamp":165542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1975,4]]},"abstract":"<jats:p>Uptake of arsenate and phosphate by Streptococcus faecalis 9790 is strictly dependent on concurrent energy metabolism and essentially unidirectional. targinine supports uptake only in presence of glycerol or related substances; glycerol is not directly involved in transport but depletes the cellular orthophosphate pool and thus relieves feedback inhibition of transport. Uptake of phosphate and arsenate is stimulated by K+ and by other permeant cations. The results suggest that electroneutrality is preserved by compensatory movement of either H+ or OH minus. Ionophores and N,N'-dicyclohexylcarbodiimide, which prevent establishment of a proton motive force, block the accumulation of thiomethylgalactoside and of threonine but not that of arsenate or phosphate. We conclude that arsenate accumulation requires adenosine 5'-triphosphate but is not driven by the proton-motive force. However, conditions and reagents that lower the cytoplasmic pH do inhibit accumulation of arsenate and phosphate, suggesting that uptake depends on the capacity of the cells to maintain a neutral or alkaline cytoplasm. We therefore propose that phosphate accumulation is an electroneutral exchange for OH driven by adenosine 5'-triphosphate or by a metabolite thereof. Accumulation of aspartate and glutamate also requires adenosine 5'-triphosphate but not the proton-motive force and may involve a similar mechanism.<\/jats:p>","DOI":"10.1128\/jb.122.1.266-277.1975","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T15:08:25Z","timestamp":1578064105000},"page":"266-277","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":85,"title":["Accumulation of arsenate, phosphate, and aspartate by Sreptococcus faecalis"],"prefix":"10.1128","volume":"122","author":[{"given":"F M","family":"Harold","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Spitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1016\/S0021-9258(19)45584-1","article-title":"Carbodiimide-resistant membrane adenosine triphosphatase in mutants of Streptococcus faecalis. I. Studies of the mechanism of resistance","volume":"247","author":"Abrams A.","year":"1972","unstructured":"Abrams , A. , J. B. Smith , and C. Baron . 1972 . Carbodiimide-resistant membrane adenosine triphosphatase in mutants of Streptococcus faecalis. I. Studies of the mechanism of resistance . J. Biol. Chem. 247 : 1484 - 1488 .","journal-title":"J. Biol. Chem."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"5225","DOI":"10.1016\/S0021-9258(19)43593-X","article-title":"Accumulation of neutral amino acids by Streptococcus faecalis: energy coupling by a proton-motive force","volume":"248","author":"Asghar S. S.","year":"1973","unstructured":"Asghar , S. S. , E. Levin , and F. M. Harold . 1973 . Accumulation of neutral amino acids by Streptococcus faecalis: energy coupling by a proton-motive force . J. Biol. Chem. 248 : 5225 - 5233 .","journal-title":"J. Biol. Chem."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/j.1749-6632.1958.tb36855.x","article-title":"Organization of red cell glycolytic enzymes: cell coat phosphorus transfer","volume":"75","author":"Bartlett G. R.","year":"1958","unstructured":"Bartlett . G. R. 1958 . Organization of red cell glycolytic enzymes: cell coat phosphorus transfer . Ann. N. Y. Acad. Sci. 75 : 110 - 114 .","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1073\/pnas.70.5.1514","article-title":"Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli","volume":"70","author":"Berger E. A.","year":"1973","unstructured":"Berger , E. A. 1973 . Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli . Proc. Natl. Acad. Sci. U.S.A. 70 : 1514 - 1518 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"7747","DOI":"10.1016\/S0021-9258(19)42031-0","article-title":"Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases of Escherichia coli","volume":"249","author":"Berger E. A.","year":"1974","unstructured":"Berger , E. A. , and L. A. Heppel . 1974 . Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases of Escherichia coli . J. Biol. Chem. 249 : 7747 - 7755 .","journal-title":"J. Biol. Chem."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/0005-2736(72)90077-6","article-title":"The mechanism of inhibition of anaerobic phosphate uptake by fatty acids in yeast","volume":"290","author":"Borst-Pauwels G. W. F. H.","year":"1972","unstructured":"Borst-Pauwels , G. W. F. H. , and J. Dobbelmann . 1972 . The mechanism of inhibition of anaerobic phosphate uptake by fatty acids in yeast . Biochim. Biophys. Acta 290 : 348 - 354 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_7","first-page":"5293","article-title":"Phosphate transport in rat liver mitochondria: kinetics and energy requirements","volume":"249","author":"Coty W. A.","year":"1974","unstructured":"Coty , W. A. , and P. Pedersen . 1974 . Phosphate transport in rat liver mitochondria: kinetics and energy requirements . J. Biol. Chem. 249 : 5293 - 5298 .","journal-title":"J. Biol. Chem."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1128\/jb.120.1.139-146.1974","article-title":"Energetics of glycylglycine transport in Escherichia coli","volume":"120","author":"Cowell J. L.","year":"1974","unstructured":"Cowell , J. L. 1974 . Energetics of glycylglycine transport in Escherichia coli . J. Bacteriol. 120 : 139 - 146 .","journal-title":"J. Bacteriol."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1128\/jb.120.1.295-303.1974","article-title":"Mechanism of energy coupling for transport of D-ribose in Escherichia coli","volume":"120","author":"Curtis S. J.","year":"1974","unstructured":"Curtis , S. J. 1974 . Mechanism of energy coupling for transport of D-ribose in Escherichia coli . J. Bacteriol. 120 : 295 - 303 .","journal-title":"J. Bacteriol."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1128\/br.30.4.772-794.1966","article-title":"Inorganic polyphosphates in biology: structure, metabolism and function","volume":"30","author":"Harold F. M.","year":"1966","unstructured":"Harold , F. M. 1966 . Inorganic polyphosphates in biology: structure, metabolism and function . Bacteriol. Rev. 30 : 772 - 794 .","journal-title":"Bacteriol. Rev."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1128\/br.36.2.172-230.1972","article-title":"Conservation and transformation of energy by bacterial membranes","volume":"36","author":"Harold F. M.","year":"1972","unstructured":"Harold , F. M. 1972 . Conservation and transformation of energy by bacterial membranes . Bacteriol. Rev. 36 : 172 - 230 .","journal-title":"Bacteriol. Rev."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0070-2161(08)60183-5","article-title":"Cation transport in bacteria: K+, Na+ and H+","volume":"5","author":"Harold F. M.","year":"1974","unstructured":"Harold , F. M. , and K. Altendorf . 1974 . Cation transport in bacteria: K+, Na+ and H+ . Curr. Top. Membr. Transp. 5 : 1 - 50 .","journal-title":"Curr. Top. Membr. Transp."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1128\/jb.91.6.2257-2262.1966","article-title":"Interaction of arsenate with phosphate-transport systems in wildtype and mutant Streptococcus faecalis","volume":"91","author":"Harold F. M.","year":"1966","unstructured":"Harold , F. M. , and J. R. Baarda . 1966 . Interaction of arsenate with phosphate-transport systems in wildtype and mutant Streptococcus faecalis . J. Bacteriol. 91 : 2257 - 2262 .","journal-title":"J. Bacteriol."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1128\/jb.94.1.53-60.1967","article-title":"Gramicidin, valinomycin, and cation permeability of Streptococcus faecalis","volume":"94","author":"Harold F. M.","year":"1967","unstructured":"Harold , F. M. , and J. R. Baarda . 1967 . Gramicidin, valinomycin, and cation permeability of Streptococcus faecalis . J. Bacteriol. 94 : 53 - 60 .","journal-title":"J. Bacteriol."},{"key":"p_15","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1128\/jb.95.3.816-823.1968","article-title":"Effects of nigericin and monactin on cation permeability of Streptococcus faecalis and metabolic capacities of potassium-depleted cells","volume":"95","author":"Harold F. M.","year":"1968","unstructured":"Harold , F. M. , and J. R. Baarda . 1968 . Effects of nigericin and monactin on cation permeability of Streptococcus faecalis and metabolic capacities of potassium-depleted cells . J. Bacteriol. 95 : 816 - 823 .","journal-title":"J. Bacteriol."},{"key":"p_16","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1128\/jb.96.6.2025-2034.1968","article-title":"Inhibition of membrane transport in Streptococcus faecalis by uncouplers of oxidative phosphorylation and its relationship to proton conduction","volume":"96","author":"Harold F. M.","year":"1968","unstructured":"Harold , F. M. , and J. R. Baarda . 1968 . Inhibition of membrane transport in Streptococcus faecalis by uncouplers of oxidative phosphorylation and its relationship to proton conduction . J. Bacteriol. 96 : 2025 - 2034 .","journal-title":"J. Bacteriol."},{"key":"p_17","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.1016\/S0021-9258(19)78220-9","article-title":"Inhibition of membrane-bound adenosine triphosphatase and of cation transport in Streptococcus faecalis by N,N'-dicyclohexylcarbodiimide","volume":"244","author":"Harold F. M.","year":"1969","unstructured":"Harold , F. M. , J. R. Baarda , C. Baron , and A. Abrams . 1969 . Inhibition of membrane-bound adenosine triphosphatase and of cation transport in Streptococcus faecalis by N,N'-dicyclohexylcarbodiimide . J. Biol. Chem. 244 : 2261 - 2268 .","journal-title":"J. Biol. Chem."},{"key":"p_18","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1128\/jb.101.1.152-159.1970","article-title":"Extrusion of sodium and hydrogen ions as the primary process in potassium ion accumulation by Streptococcus faecalis","volume":"101","author":"Harold F. M.","year":"1970","unstructured":"Harold , F. M. , J. R Baarda , and E. Pavlasova . 1970 . Extrusion of sodium and hydrogen ions as the primary process in potassium ion accumulation by Streptococcus faecalis . J. Bacteriol. 101 : 152 - 159 .","journal-title":"J. Bacteriol."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"3145","DOI":"10.1016\/S0021-9258(18)97300-X","article-title":"A mutant of Streptococcus faecalis defective in phosphate uptake","volume":"240","author":"Harold F. M.","year":"1965","unstructured":"Harold , F. M. , R. L. Harold , and A. Abrams . 1965 . A mutant of Streptococcus faecalis defective in phosphate uptake . J. Biol. Chem. 240 : 3145 - 3153 .","journal-title":"J. Biol. Chem."},{"key":"p_20","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1128\/jb.117.3.1141-1148.1974","article-title":"Lactic acid translocation: terminal step in glycolysis by Streptococcus faecalis","volume":"117","author":"Harold F. M.","year":"1974","unstructured":"Harold , F. M. , and E. Levin . 1974 . Lactic acid translocation: terminal step in glycolysis by Streptococcus faecalis . J. Bacteriol. 117 : 1141 - 1148 .","journal-title":"J. Bacteriol."},{"key":"p_21","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/BF01868093","article-title":"Cation transport and electrogenesis by Streptococcus faecalis. I. The membrane potential","volume":"8","author":"Harold F. M.","year":"1972","unstructured":"Harold , F. M. , and D. Papineau . 1972 . Cation transport and electrogenesis by Streptococcus faecalis. I. The membrane potential . J. Membr. Biol. 8 : 27 - 44 .","journal-title":"J. Membr. Biol."},{"key":"p_22","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/BF01868094","article-title":"Cation transport and electrogenesis by Streptococcus faecalis. II. Proton and sodium extrusion","volume":"8","author":"Harold F. M.","year":"1972","unstructured":"Harold , F. M. , and D. Papineau . 1972 . Cation transport and electrogenesis by Streptococcus faecalis. II. Proton and sodium extrusion . J. Membr. Biol. 8 : 45 - 62 .","journal-title":"J. Membr. Biol."},{"key":"p_23","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0005-2736(70)90011-8","article-title":"A transmembrane pH gradient in Streptococcus faecalis: origin, and dissipation by proton conductors and N,N'- dicyclohexylcarbodiimide","volume":"196","author":"Harold F. M.","year":"1970","unstructured":"Harold , F. M. , E. Pavlosova , and J. R. Baarda . 1970 . A transmembrane pH gradient in Streptococcus faecalis: origin, and dissipation by proton conductors and N,N'- dicyclohexylcarbodiimide . Biochim. Biophys. Acta 196 : 235 - 244 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_24","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1017\/S0033583500000883","article-title":"Ion transport across thin lipid membranes: a critical discussion of mechanisms in selected systems","volume":"5","author":"Haydon D. A.","year":"1972","unstructured":"Haydon , D. A. , and S. B. Hladky . 1972 . Ion transport across thin lipid membranes: a critical discussion of mechanisms in selected systems . Quart. Rev. Biophys. 5 : 187 - 282 .","journal-title":"Quart. Rev. Biophys."},{"key":"p_25","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/0005-2728(72)90239-3","article-title":"The effect of N,N'-dicyclohexylcarbodiimide on anaerobic and aerobic phosphate uptake by baker's yeast","volume":"283","author":"Huygen P. L. M.","year":"1972","unstructured":"Huygen , P. L. M. , and G. W. F. H. Borst-Pauwels . 1972 . The effect of N,N'-dicyclohexylcarbodiimide on anaerobic and aerobic phosphate uptake by baker's yeast . Biochim. Biophys. Acta 283 : 234 - 238 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_26","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/0006-291X(72)90455-X","article-title":"Galactoside accumulation associated with ion movements in Streptococcus lactis","volume":"49","author":"Kashket E. R.","year":"1972","unstructured":"Kashket , E. R. , and T. H. Wilson . 1972 . Galactoside accumulation associated with ion movements in Streptococcus lactis . Biochem. Biophys. Res. Commun. 49 : 615 - 620 .","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"p_27","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1128\/jb.109.2.784-789.1972","article-title":"Role of metabolic energy in the transport of ,B-galactosides by Streptococcus lactis","volume":"109","author":"Kashket E. R.","year":"1972","unstructured":"Kashket , E. R. , and T. H. Wilson . 1972 . Role of metabolic energy in the transport of ,B-galactosides by Streptococcus lactis . J. Bacteriol. 109 : 784 - 789 .","journal-title":"J. Bacteriol."},{"key":"p_28","doi-asserted-by":"crossref","first-page":"2866","DOI":"10.1073\/pnas.70.10.2866","article-title":"Proton-coupled accumulation of galactoside in Streptococcus lactis 7962","volume":"70","author":"Kashket E. R.","year":"1973","unstructured":"Kashket , E. R. , and T. H. Wilson . 1973 . Proton-coupled accumulation of galactoside in Streptococcus lactis 7962 . Proc. Natl. Acad. Sci. U.S.A. 70 : 2866 - 2869 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_29","first-page":"119","article-title":"Metabolite transport in mitochondria: an example for intracellular membrane function","volume":"6","author":"Klingenberg M.","year":"1970","unstructured":"Klingenberg , M. 1970 . Metabolite transport in mitochondria: an example for intracellular membrane function . Essays Biochem. 6 : 119 - 159 .","journal-title":"Essays Biochem."},{"key":"p_30","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/0006-291X(74)90591-9","article-title":"Estimation of membrane potentials in Streptococcus faecalis by means of a fluorescent probe","volume":"57","author":"Laris L. C.","year":"1974","unstructured":"Laris , L. C. , and H. A. Pershadsingh . 1974 . Estimation of membrane potentials in Streptococcus faecalis by means of a fluorescent probe . Biochem. Biophys. Res. Commun. 57 : 620 - 626 .","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"p_31","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/0005-2736(71)90048-4","article-title":"Phosphate transport in Escherichia coli","volume":"241","author":"Medveczky N.","year":"1971","unstructured":"Medveczky , N. , and H. Rosenberg . 1971 . Phosphate transport in Escherichia coli . Biochim. Biophys. Acta 241 : 494 - 506 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_32","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1111\/j.1469-185X.1966.tb01501.x","article-title":"Chemiosmotic coupling in oxidative and photosynthetic phosphorylation","volume":"41","author":"Mitchell P.","year":"1966","unstructured":"Mitchell , P. 1966 . Chemiosmotic coupling in oxidative and photosynthetic phosphorylation . Biol. Rev. Cambridge Phil. Soc. 41 : 445 - 502 .","journal-title":"Biol. Rev. Cambridge Phil. Soc."},{"key":"p_33","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/BF01516051","article-title":"Performance and conservation of osmotic work by proton-coupled solute porter systems","volume":"4","author":"Mitchell P.","year":"1973","unstructured":"Mitchell , P. 1973 . Performance and conservation of osmotic work by proton-coupled solute porter systems . Bioenergetics 4 : 63 - 91 .","journal-title":"Bioenergetics"},{"key":"p_34","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1111\/j.1432-1033.1974.tb03510.x","article-title":"Mechanisms of energy coupling to the transport of amino acids by Staphylococcus aureus","volume":"44","author":"Niven D. F.","year":"1974","unstructured":"Niven , D. F. , and W. A. Hamilton . 1974 . Mechanisms of energy coupling to the transport of amino acids by Staphylococcus aureus . Eur. J. Biochem. 44 : 517 - 522 .","journal-title":"Eur. J. Biochem."},{"key":"p_35","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1111\/j.1432-1033.1970.tb01158.x","article-title":"Quantitative correlation between the distribution of anions and the pH difference across the mitochondrial membrane","volume":"17","author":"Palmieri F.","year":"1970","unstructured":"Palmieri , F. , E. Quagliariello , and M. Klingenberg . 1970 . Quantitative correlation between the distribution of anions and the pH difference across the mitochondrial membrane . Eur. J. Biochem. 17 : 230 - 238 .","journal-title":"Eur. J. Biochem."},{"key":"p_36","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/0005-2736(73)90339-8","article-title":"The transport of Krebs-cycle intermediates in Azotobacter vinelandii under various metabolic conditions","volume":"318","author":"Postma P. W.","year":"1973","unstructured":"Postma , P. W. , A. Cools , and K. VanDam . 1973 . The transport of Krebs-cycle intermediates in Azotobacter vinelandii under various metabolic conditions . Biochim. Biophys. Acta 318 : 91 - 104 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_37","doi-asserted-by":"crossref","first-page":"5261","DOI":"10.1016\/S0021-9258(18)62749-8","article-title":"Multiple transport components for dicarboxylic acids in Streptococcus faecalis","volume":"245","author":"Reid K. G.","year":"1970","unstructured":"Reid , K. G. , N. M. Utech , and J. T. Holden . 1970 . Multiple transport components for dicarboxylic acids in Streptococcus faecalis . J. Biol. Chem. 245 : 5261 - 5272 .","journal-title":"J. Biol. Chem."},{"key":"p_38","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/0005-2736(69)90069-8","article-title":"Phosphate transport in Bacillus cereus","volume":"193","author":"Rosenberg H.","year":"1969","unstructured":"Rosenberg , H. , N. Medveczky , and J. M. LaNauze . 1969 . Phosphate transport in Bacillus cereus . Biochim. Biophys. Acta 193 : 159 - 167 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_39","doi-asserted-by":"crossref","first-page":"5273","DOI":"10.1016\/S0021-9258(18)62750-4","article-title":"Properties of a dicarboxylic amino acid transport-deficient mutant of Streptococcus faecalis","volume":"245","author":"Utech N. M.","year":"1970","unstructured":"Utech , N. M. , K. G. Reid , and J. T. Holden . 1970 . Properties of a dicarboxylic amino acid transport-deficient mutant of Streptococcus faecalis . J. Biol. Chem. 245 : 5273 - 5280 .","journal-title":"J. Biol. Chem."},{"key":"p_40","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1042\/bj0490286","article-title":"The catalytic effect of molybdate on the hydrolysis of organic phosphate bonds","volume":"49","author":"Weil-Malherbe H.","year":"1951","unstructured":"Weil-Malherbe , H. , and R. H. Green . 1951 . The catalytic effect of molybdate on the hydrolysis of organic phosphate bonds . Biochem. J. 49 : 286 - 292 .","journal-title":"Biochem. J."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.122.1.266-277.1975","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.122.1.266-277.1975","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:16:19Z","timestamp":1627578979000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.122.1.266-277.1975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,4]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1975,4]]}},"alternative-id":["10.1128\/jb.122.1.266-277.1975"],"URL":"https:\/\/doi.org\/10.1128\/jb.122.1.266-277.1975","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,4]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}