{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T02:02:03Z","timestamp":1760061723542},"reference-count":5,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1975,8,1]],"date-time":"1975-08-01T00:00:00Z","timestamp":176083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1975,8]]},"abstract":"<jats:p>The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The results are in quantitative accordance with a random replication of R1 in which the replicating molecules are taken from a cytoplasmic plasmid pool and transferred back to the pool after replication. This is analogous to the results obtained by Bazaral and Helinski (1970) and Rownd (1969) for plasmids that are present in 10 to 20 copies per genome (so-called relaxed control of replication). Hence, there seem to be no difference between stringent and relaxed plasmids with respect to selection of plasmid molecules for replication. However, we cannot tell whether all R1 copies in a cell replicate during a fraction of or throughout the cell cycle. The random selction of plasmid copies for replication has to be considered when models for control of replication are constructed.<\/jats:p>","DOI":"10.1128\/jb.123.2.443-448.1975","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T15:48:16Z","timestamp":1578066496000},"page":"443-448","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Random replication of the stringent plasmid R1 in Escherichia coli K-12"],"prefix":"10.1128","volume":"123","author":[{"given":"P","family":"Gustafsson","sequence":"first","affiliation":[]},{"given":"K","family":"Nordstr\u00f6m","sequence":"additional","affiliation":[]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/BF00268231","article-title":"The replication of prophage P 1 DNA","volume":"132","author":"Abe M.","year":"1974","unstructured":"Abe , M. 1974 . The replication of prophage P 1 DNA . Mol. Gen. Genet. 132 : 63 - 72 .","journal-title":"Mol. Gen. Genet."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1021\/bi00804a029","article-title":"Rep lication of a bacterial plasmid and an episome in Esc) herichia colt","volume":"9","author":"Bazaral M.","year":"1970","unstructured":"Bazaral , M. , and D. R. Helinski . 1970 . Rep lication of a bacterial plasmid and an episome in Esc) herichia colt . Biochemistry 9 : 399 - 406 .","journal-title":"Biochemistry"},{"key":"p_3","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1128\/br.36.3.361-405.1972","article-title":"Molecular structure of bacterial plasmids","volume":"36","author":"Clowes R. C.","year":"1972","unstructured":"Clowes , R. C. 1972 . Molecular structure of bacterial plasmids . Bacteriol. Rev. 36 : 361 - 405 .","journal-title":"Bacteriol. Rev."},{"key":"p_4","doi-asserted-by":"crossref","unstructured":"Collins J. and R. P. Pritchard. 1973. between chromosome replication and F replication in Escherichia coli. J. 78:143-155.","DOI":"10.1016\/0022-2836(73)90434-8"},{"key":"p_5","doi-asserted-by":"crossref","first-page":"2706","DOI":"10.1073\/pnas.69.9.2706","article-title":"Relationship of F'lac rep chromosome replication","volume":"69","author":"Cooper S.","year":"1972","unstructured":"Cooper , S. 1972 . Relationship of F'lac rep chromosome replication . Proc. Natl. Acad 69 : 2706 - 2710 .","journal-title":"Proc. Natl. Acad"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.123.2.443-448.1975","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.123.2.443-448.1975","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:18:37Z","timestamp":1627579117000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.123.2.443-448.1975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,8]]},"references-count":5,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1975,8]]}},"alternative-id":["10.1128\/jb.123.2.443-448.1975"],"URL":"https:\/\/doi.org\/10.1128\/jb.123.2.443-448.1975","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,8]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}