{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:09:18Z","timestamp":1758280158838},"reference-count":20,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1977,3,1]],"date-time":"1977-03-01T00:00:00Z","timestamp":226022400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1977,3]]},"abstract":"<jats:p>Both the beta-lactam antibiotic, cephalexin, and the deoxyribonucleic acid synthesis inhibitor, nalidixic acid, are known to inhibit cell division in Escherichia coli and induce the formation of filaments. The biosynthesis of murein was investigated in these filaments and compared with the murein synthesized by the normally dividing rods of E. coli PAT 84. Differences were found in the extent of peptide side-chain cross-linkage. Filamentous cells had higher extents of cross-linkages in their newly synthesized murein. Quantitative analyses of the D-alanine carboxypeptidase and transpeptidase reactions in the different cells revealed that the carboxypeptidase activity of the filamentous cells was partially inhibited. These results were similar to those previously found with filaments that were obtained after growth of the thermosensitive division mutant at its restrictive temperature. We conclude that the formation of new cell ends (septa) depends on the proper balance between the activities of the D-alanine carboxypeptidase that regulates the availability of precursor doners and the transpeptidase, which catalyzes cross-linking and attachment of newly synthesized murein.<\/jats:p>","DOI":"10.1128\/jb.129.3.1593-1600.1977","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T16:11:25Z","timestamp":1578067885000},"page":"1593-1600","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Regulation of murein biosynthesis and septum formation in filamentous cells of Escherichia coli PAT 84"],"prefix":"10.1128","volume":"129","author":[{"given":"D","family":"Mirelman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Yashouv-Gan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U","family":"Schwarz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1128\/br.38.3.291-335.1974","article-title":"Interaction of penicillin with the bacterial cell. Penicillinbinding proteins and penicillin-sensitive enzymes","volume":"38","author":"Blumberg P. M.","year":"1974","unstructured":"Blumberg , P. M. , and J. L. Strominger . 1974 . Interaction of penicillin with the bacterial cell. Penicillinbinding proteins and penicillin-sensitive enzymes . Bacteriol. Rev. 38 : 291 - 335 .","journal-title":"Bacteriol. Rev."},{"key":"p_2","first-page":"390","article-title":"Sur l'identite de la mucoendopeptidase et de la carboxypeptidase I d'Escherichia coli, enzymes hydrolysant des liaisons de configuration D-D et inhibee par la penicilline. C.R","volume":"269","author":"Bogdanovsky D.","year":"1969","unstructured":"Bogdanovsky , D. , E. Bricas , and P. Dezelee . 1969 . Sur l'identite de la mucoendopeptidase et de la carboxypeptidase I d'Escherichia coli, enzymes hydrolysant des liaisons de configuration D-D et inhibee par la penicilline. C.R . Acad. Sci. (Par 15) Ser D. 269 : 390 - 393 .","journal-title":"Acad. Sci. (Par 15) Ser D."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1128\/jb.119.1.303-324.1974","article-title":"Septum formation in Escherichia coli: characterization of septal structure and the effects of antibiotics on cell division","volume":"119","author":"Burdett I. D. J.","year":"1974","unstructured":"Burdett , I. D. J. , and R. G. E. Murray . 1974 . Septum formation in Escherichia coli: characterization of septal structure and the effects of antibiotics on cell division . J. Bacteriol. 119 : 303 - 324 .","journal-title":"J. Bacteriol."},{"key":"p_4","unstructured":"Goss W. A. and T. M. Cook. 1975. Nalidixic acid-mode"},{"key":"p_5","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1093\/infdis\/128.2.211","article-title":"Comparison of the responses ofEscherichia coli and Proteus mirabilis to seven f3-lactam antibiotics","volume":"128","author":"Greenwood D.","year":"1973","unstructured":"Greenwood , D. , and F. O'Grady . 1973 . Comparison of the responses ofEscherichia coli and Proteus mirabilis to seven f3-lactam antibiotics . J. Infect. Dis. 128 : 211 - 222 .","journal-title":"J. Infect. Dis."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"3470","DOI":"10.1016\/S0021-9258(17)33462-2","article-title":"Evidence for the involvement of an outer membrane protein in DNA initiation","volume":"251","author":"Gudas L. J.","year":"1976","unstructured":"Gudas , L. J. , R. James , and A. B. Pardee , 1976 . Evidence for the involvement of an outer membrane protein in DNA initiation . J. Biol. Chem. 251 : 3470 - 3479 .","journal-title":"J. Biol. Chem."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/0022-2836(76)90240-0","article-title":"DNA synthesis inhibition and the induction of protein X in Escherichia coli","volume":"101","author":"Gudas L. J.","year":"1976","unstructured":"Gudas , L. J. , and A. B. Pardee . 1976 . DNA synthesis inhibition and the induction of protein X in Escherichia coli . J. Mol. Biol. 101 : 459 - 477 .","journal-title":"J. Mol. Biol."},{"key":"p_8","doi-asserted-by":"crossref","unstructured":"Hartmann R. J.-V. Holtje and U. Schwarz. 1972. Targets of penicillin action in Escherichia coli. Natr Lno)254649 ture (London) 235:426-429.","DOI":"10.1038\/235426a0"},{"key":"p_9","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1101\/SQB.1968.033.01.077","article-title":"Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division","volume":"33","author":"Hirota Y.","year":"1968","unstructured":"Hirota , Y. , A. Ryter , and F. Jacob . 1968 . Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division . Cold Spring Harbor Symp. Quant. Biol. 33 : 677 - 687 .","journal-title":"Cold Spring Harbor Symp. Quant. Biol."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1073\/pnas.55.3.656","article-title":"Glycopeptide transpeptidase and D-alanine carboxypeptidase: penicillin-sensitive enzymatic reactions","volume":"55","author":"Izaki K.","year":"1966","unstructured":"Izaki , K. , M. Matsuhashi , and J. L. Strominger . 1966 . Glycopeptide transpeptidase and D-alanine carboxypeptidase: penicillin-sensitive enzymatic reactions . Proc. Natl. Acad. Sci. U.S.A. 55 : 656 - 663 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/0022-2836(73)90198-8","article-title":"Maturation of the head of bacteriophage T4","volume":"80","author":"Laemmli U. K.","year":"1973","unstructured":"Laemmli , U. K. , and M. Favre . 1973 . Maturation of the head of bacteriophage T4 . J. Mol. Biol. 80 : 575 - 599 .","journal-title":"J. Mol. Biol."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurements with the Folin phenol reagent","volume":"193","author":"Lowry 0.","year":"1951","unstructured":"Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurements with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 .","journal-title":"J. Biol. Chem."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"3355","DOI":"10.1073\/pnas.69.11.3355","article-title":"Role of the penicillin-sensitive transpeptidation reaction in the attachment of newly synthesized peptidoglycan to cell walls .of Micrococcus Iuteus","volume":"69","author":"Mirelman D.","year":"1972","unstructured":"Mirelman , D. , R. Bracha , and N. Sharon . 1972 . Role of the penicillin-sensitive transpeptidation reaction in the attachment of newly synthesized peptidoglycan to cell walls .of Micrococcus Iuteus . Proc. Natl. Acad. Sci. U.S.A. 69 : 3355 - 3359 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1021\/bi00654a001","article-title":"Peptidoglycan biosynthesis in a thermosensitive division mutant of Escherichia coli","volume":"15","author":"Mirelman D.","year":"1976","unstructured":"Mirelman , D. , Y. Yashouv-Gan , and U. Schwarz . 1976 . Peptidoglycan biosynthesis in a thermosensitive division mutant of Escherichia coli . Biochemistry 15 : 1781 - 1790 .","journal-title":"Biochemistry"},{"key":"p_15","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/j.1432-1033.1974.tb03287.x","article-title":"Sensitivity to ampicillin and cephalothin of enzymes involved in wall peptide crosslinking in Escherichia coli K12, strain 44","volume":"41","author":"Nguyen-Disteche M.","year":"1974","unstructured":"Nguyen-Disteche , M. , J. J. Pollock , J. M. Ghuysen , J. Puig , P. Reynolds , H. R. Perkins , J. Coyette , and M. R. J. Salton . 1974 . Sensitivity to ampicillin and cephalothin of enzymes involved in wall peptide crosslinking in Escherichia coli K12, strain 44 . Eur. J. Biochem. 41 : 457 - 463 .","journal-title":"Eur. J. Biochem."},{"key":"p_16","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1128\/br.38.2.199-221.1974","article-title":"Control of cell division in bacteria","volume":"38","author":"Slater M.","year":"1974","unstructured":"Slater , M. , and M. Schaechter . 1974 . Control of cell division in bacteria . Bacteriol. Rev. 38 : 199 - 221 .","journal-title":"Bacteriol. Rev."},{"key":"p_17","doi-asserted-by":"crossref","first-page":"2999","DOI":"10.1073\/pnas.72.8.2999","article-title":"Distinct penicillin binding proteins involved in the division, elongation and shape of Escherichia coli K,2","volume":"72","author":"Spratt B. G.","year":"1975","unstructured":"Spratt , B. G. 1975 . Distinct penicillin binding proteins involved in the division, elongation and shape of Escherichia coli K,2 . Proc. Natl. Acad. Sci. U.S.A. 72 : 2999 - 3003 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_18","first-page":"179","article-title":"Penicillin-sensitive enzymatic reactions in bacterial cell wall synthesis","volume":"64","author":"Strominger J. L.","year":"1970","unstructured":"Strominger , J. L. 1970 . Penicillin-sensitive enzymatic reactions in bacterial cell wall synthesis . Harvey Lect. 64 : 179 - 213 .","journal-title":"Harvey Lect."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/S0021-9258(17)33895-4","article-title":"Purification to homogeneity and properties of two D-alanine carboxypeptidases I from Escherichia coli","volume":"251","author":"Tamura T.","year":"1976","unstructured":"Tamura , T. , Y. Imae , and J. L. Strominger . 1976 . Purification to homogeneity and properties of two D-alanine carboxypeptidases I from Escherichia coli . J. Biol. Chem. 251 : 414 - 423 .","journal-title":"J. Biol. Chem."},{"key":"p_20","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/0022-2836(71)90037-4","article-title":"DNA synthesis in nucleotide-permeable Escherichia coli cells","volume":"58","author":"Vosberg H. P.","year":"1971","unstructured":"Vosberg , H. P. and H. Hoffmann-Berling . 1971 . DNA synthesis in nucleotide-permeable Escherichia coli cells . J. Mol. Biol. 58 : 739 - 753 .","journal-title":"J. Mol. Biol."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.129.3.1593-1600.1977","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.129.3.1593-1600.1977","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:24:35Z","timestamp":1627579475000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.129.3.1593-1600.1977"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,3]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1977,3]]}},"alternative-id":["10.1128\/jb.129.3.1593-1600.1977"],"URL":"https:\/\/doi.org\/10.1128\/jb.129.3.1593-1600.1977","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,3]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}