{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:27Z","timestamp":1695772827340},"reference-count":32,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1979,7,1]],"date-time":"1979-07-01T00:00:00Z","timestamp":299635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1979,7]]},"abstract":"<jats:p>Deoxyribonucleic acid repair synthesis was studied in one wild-type and two mutant strains of Bacillus subtilis that are defective in excision of pyrimidine dimers. The cells were irradiated with ultraviolet light, and 6-(p-hydroxyphenyl-azo)-uracil was used to block replicative synthesis, allowing only repair synthesis. One of the mutations (uvs-42) resulted in a severe inhibition of incision, dimer excision, and repair synthesis. In contrast, the other mutant (uvr-1) slowly incised and excised dimers and did repair synthesis in patches which appear to be several-fold longer than those in the wild-type strain, apparently because large gaps are produced at excision sites. The results indicate that the primary defect in uvs-42 cells is in initiation of dimer excision, whereas the uvr-1 mutation appears to be a defect in the exonuclease normally used to complete dimer excision.<\/jats:p>","DOI":"10.1128\/jb.139.1.239-246.1979","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T16:34:13Z","timestamp":1578069253000},"page":"239-246","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Gap-filling repair synthesis induced by ultraviolet light in a Bacillus subtilis Uvr- mutant"],"prefix":"10.1128","volume":"139","author":[{"given":"C T","family":"Hadden","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1128\/JB.81.5.741-746.1961","article-title":"Requirements for transformation in Bacillus subtilis","volume":"81","author":"Anagnostopoulos C.","year":"1961","unstructured":"Anagnostopoulos , C. , and J. Spizizen . 1961 . Requirements for transformation in Bacillus subtilis . J. Bacteriol. 81 : 741 - 746 .","journal-title":"J. Bacteriol."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1128\/jb.108.3.1250-1256.1971","article-title":"Evidence suggestive of compartmentalization of deoxyribonucleic acid-synthesizing systems in freeze-treated Bacillus subtilis","volume":"108","author":"Billen D.","year":"1971","unstructured":"Billen , D. , L B . Carreira , C. T. Hadden , and S. J. Silverstein . 1971 . Evidence suggestive of compartmentalization of deoxyribonucleic acid-synthesizing systems in freeze-treated Bacillus subtilis . J. Bacteriol. 108 : 1250 - 1256 .","journal-title":"J. Bacteriol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1128\/JB.94.5.1538-1545.1967","article-title":"Deoxyribonucleic acid repair replication after ultraviolet light or X-ray exposure of bacteria","volume":"94","author":"Billen D.","year":"1967","unstructured":"Billen , D. , R. R. Hewitt , T. Lapthisophon , and P. M. Achey . 1967 . Deoxyribonucleic acid repair replication after ultraviolet light or X-ray exposure of bacteria . J. Bacteriol. 94 : 1538 - 1545 .","journal-title":"J. Bacteriol."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"2733","DOI":"10.1016\/S0021-9258(18)69770-4","article-title":"Thermal conversion of nonpriming deoxyribonucleic acid to primer","volume":"234","author":"Bollum F. J.","year":"1959","unstructured":"Bollum , F. J. 1959 . Thermal conversion of nonpriming deoxyribonucleic acid to primer . J. Biol. Chem. 234 : 2733 - 2734 .","journal-title":"J. Biol. Chem."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1073\/pnas.51.2.293","article-title":"Release of ultraviolet light-induced thymine dimers from DNA in E. coli K-12","volume":"51","author":"Boyce R. P.","year":"1964","unstructured":"Boyce , R. P. , and P. Howard-Flanders . 1964 . Release of ultraviolet light-induced thymine dimers from DNA in E. coli K-12 . Proc. Natl. Acad. Sci. U.S.A. 51 : 293 - 300 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1073\/pnas.67.3.1454","article-title":"6-(p-Hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phageinfected Bacillus subtilis","volume":"67","author":"Brown N. C.","year":"1970","unstructured":"Brown , N. C. 1970 . 6-(p-Hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phageinfected Bacillus subtilis . Proc. Natl. Acad. Sci. U.S.A. 67 : 1454 - 1461 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0022-2836(71)90409-8","article-title":"Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: different effect on repair and semiconservative synthesis in Bacillus subtilis","volume":"59","author":"Brown N. C.","year":"1971","unstructured":"Brown , N. C. 1971 . Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: different effect on repair and semiconservative synthesis in Bacillus subtilis . J. Mol. Biol. 59 : 1 - 16 .","journal-title":"J. Mol. Biol."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/S0076-6879(71)21015-6","article-title":"The excision of pyrimidine dimers (the detection of dimers in small amounts)","volume":"21","author":"Carrier W. L.","year":"1971","unstructured":"Carrier , W. L. , and R. B. Setlow . 1971 . The excision of pyrimidine dimers (the detection of dimers in small amounts) . Methods Enzymol. 21 : 230 - 237 .","journal-title":"Methods Enzymol."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1751-1097.1971.tb06094.x","article-title":"Absence of repair replication following ultraviolet irradiation of an excision-deficient mutant of Escherichia coli","volume":"13","author":"Cooper P. K.","year":"1971","unstructured":"Cooper , P. K. , and P. C. Hanawalt . 1971 . Absence of repair replication following ultraviolet irradiation of an excision-deficient mutant of Escherichia coli . Photochem. Photobiol. 13 : 83 - 85 .","journal-title":"Photochem. Photobiol."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0022-2836(72)90381-6","article-title":"Heterogeneity of patch size in repair-replicated DNA in Escherichia coli","volume":"67","author":"Cooper P. K.","year":"1972","unstructured":"Cooper , P. K. , and P. C. Hanawalt . 1972 . Heterogeneity of patch size in repair-replicated DNA in Escherichia coli . J. Mol. Biol. 67 : 1 - 10 .","journal-title":"J. Mol. Biol."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"7688","DOI":"10.1016\/S0021-9258(19)43246-8","article-title":"Further genetic and enzymological characterization of the three Bacillus subtilis deoxyribonucleic acid polymerases","volume":"248","author":"Gas K. B.","year":"1973","unstructured":"Gas &, K. B. , and N. R. Cozzarelli . 1973 . Further genetic and enzymological characterization of the three Bacillus subtilis deoxyribonucleic acid polymerases . J. Biol. Chem. 248 : 7688 - 7700 .","journal-title":"J. Biol. Chem."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1128\/JB.128.1.317-324.1976","article-title":"Postirradiation recovery dependent on the uvr- I locus in Bacillus subtilis","volume":"128","author":"Hadden C. T.","year":"1976","unstructured":"Hadden , C. T. 1976 . Postirradiation recovery dependent on the uvr- I locus in Bacillus subtilis . J. Bacteriol. 128 : 317 - 324 .","journal-title":"J. Bacteriol."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1128\/JB.139.1.247-255.1979","article-title":"Pyrimidine dimer excision in a Bacillu 8 subtilis Uvr- mutant","volume":"139","author":"Hadden C. T.","year":"1979","unstructured":"Hadden , C. T. 1979 . Pyrimidine dimer excision in a Bacillu 8 subtilis Uvr- mutant . J. Bacteriol. 139 : 247 - 255 .","journal-title":"J. Bacteriol."},{"key":"p_14","unstructured":"Harford N. I. Samojilenko and M. Mergeay. 1973. Isolation and characterization of recombination-defective mutants of Bacillus subitilis p. 241-268. In L. J. Archer (ed.) Bacterial transformation. Academic Press London."},{"key":"p_15","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1128\/jb.103.2.295-301.1970","article-title":"Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis","volume":"103","author":"Hoch J. A.","year":"1970","unstructured":"Hoch , J. A. , and C. Anagnostopoulos . 1970 . Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis . J. Bacteriol. 103 : 295 - 301 .","journal-title":"J. Bacteriol."},{"key":"p_16","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1128\/jb.93.6.1925-1937.1967","article-title":"Transformation and transduction in recombination-defective mutants of Bacillus subtilis","volume":"93","author":"Hoch J. A.","year":"1967","unstructured":"Hoch , J. A. , M. Barat , and C. Anagnostopoulos . 1967 . Transformation and transduction in recombination-defective mutants of Bacillus subtilis . J. Bacteriol. 93 : 1925 - 1937 .","journal-title":"J. Bacteriol."},{"key":"p_17","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/224495a0","article-title":"Excision of thymine dimers and other mismatched sequences by DNA polymerase of Escherichia coli","volume":"224","author":"Kelly R. B.","year":"1969","unstructured":"Kelly , R. B. , M. R. Atkinson , J. A. Huberman , and A. Kornberg . 1969 . Excision of thymine dimers and other mismatched sequences by DNA polymerase of Escherichia coli . Nature (London) 224 : 495 - 501 .","journal-title":"Nature (London)"},{"key":"p_18","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.1073\/pnas.71.8.2973","article-title":"Mechanism of inhibition of Bacillus subtilis DNA polymerase III by the arylhydrazinopyrimidine antimicrobial agents","volume":"71","author":"Low R. L.","year":"1974","unstructured":"Low , R. L. , S. A. Rashbaum , and N. R. Cozzarelli . 1974 . Mechanism of inhibition of Bacillus subtilis DNA polymerase III by the arylhydrazinopyrimidine antimicrobial agents . Proc. Natl. Acad. Sci. U.S.A. 71 : 2973 - 2977 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/0005-2787(75)90105-7","article-title":"Retardation time measurements on replicating Bacillus subtilis chromosomes: effect of EDTA concentration","volume":"378","author":"Muller W. A.","year":"1975","unstructured":"Muller , W. A. , and L , C. Klotz . 1975 . Retardation time measurements on replicating Bacillus subtilis chromosomes: effect of EDTA concentration . Biochim. Biophys. Acta 378 : 171 - 185 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_20","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0027-5107(69)90025-6","article-title":"Inactivation of transforming DNA by ultraviolet irradiation: a study with ultraviolet-sensitive mutants of Bacillus subtilis","volume":"7","author":"Munakata N.","year":"1969","unstructured":"Munakata , N. , and Y. Ikeda . 1969 . Inactivation of transforming DNA by ultraviolet irradiation: a study with ultraviolet-sensitive mutants of Bacillus subtilis . Mutat. Res. 7 : 133 - 139 .","journal-title":"Mutat. Res."},{"key":"p_21","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1128\/jb.111.1.192-198.1972","article-title":"Genetically controlled removal of \"spore photoproduct","volume":"111","author":"Munakata N.","year":"1972","unstructured":"Munakata , N. , and C. S. Rupert . 1972 . Genetically controlled removal of \"spore photoproduct \" from deoxyribonucleic acid of ultraviolet-irradiated Bacillus subtilis J. Bacteriol. 111 : 192 - 198 .","journal-title":"J. Bacteriol."},{"key":"p_22","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/S0022-2836(64)80216-3","article-title":"Evidence for repair-replication of ultraviolet-damaged DNA in bacteria","volume":"9","author":"Pettijohn D.","year":"1964","unstructured":"Pettijohn , D. , and P. Hanawalt . 1964 . Evidence for repair-replication of ultraviolet-damaged DNA in bacteria . J. Mol. Biol. 9 : 395 - 410 .","journal-title":"J. Mol. Biol."},{"key":"p_23","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0022-2836(65)80239-X","article-title":"Repair of damage induced by a monofunctional alkylating agent in a transformable, ultraviolet-sensitive strain of Bacillus subtili.s","volume":"14","author":"Reiter H.","year":"1965","unstructured":"Reiter , H. , and B. Strauss . 1965 . Repair of damage induced by a monofunctional alkylating agent in a transformable, ultraviolet-sensitive strain of Bacillus subtili.s . J. Mol. Biol. 14 : 179 - 194 .","journal-title":"J. Mol. Biol."},{"key":"p_24","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1128\/JB.136.1.444-448.1978","article-title":"Dimer excision and repair replication patch size in a recL152 mutant of Escherichia coli K-12","volume":"136","author":"Rothman R. IL","year":"1978","unstructured":"Rothman , R. IL 1978 . Dimer excision and repair replication patch size in a recL152 mutant of Escherichia coli K-12 . J. Bacteriol. 136 : 444 448.","journal-title":"J. Bacteriol."},{"key":"p_25","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/BF00272805","article-title":"Defective excision and postreplication repair of UV-damaged DNA in a recL mutant strain of E. coli K-12","volume":"155","author":"Rothman R. H.","year":"1977","unstructured":"Rothman , R. H. , and A. J. Clark . 1977 . Defective excision and postreplication repair of UV-damaged DNA in a recL mutant strain of E. coli K-12 . Mol. Gen. Genet. 155 : 267 - 277 .","journal-title":"Mol. Gen. Genet."},{"key":"p_26","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/BF00269129","article-title":"Survival, mutation, and capacity to repair single-strand DNA breaks after gamma irradiation in different Exr- strains of Escherichia coli","volume":"119","author":"Sedgwick S. G.","year":"1972","unstructured":"Sedgwick , S. G. , and B. A. Bridges . 1972 . Survival, mutation, and capacity to repair single-strand DNA breaks after gamma irradiation in different Exr- strains of Escherichia coli . Mol. Gen. Genet. 119 : 93 - 102 .","journal-title":"Mol. Gen. Genet."},{"key":"p_27","doi-asserted-by":"crossref","unstructured":"Seeberg E. and W. D. Rupp. 1975. Effect of mutation in lig and polA on UV-induced strand cutting in a uvrC strain of Escherichia coli p. 439-442. In P. C. Hanawalt and R. B. Setlow (ed.) Molecular mechanisms for the repair of DNA. Plenum Publishing Corp. New York.","DOI":"10.1007\/978-1-4684-2898-8_3"},{"key":"p_28","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1128\/jb.125.3.787-795.1976","article-title":"Excision repair of ultraviolet-irradiated deoxyribonucleic acid in plasmnolyzed cells of Escherichia coli","volume":"125","author":"Seeberg E.","year":"1976","unstructured":"Seeberg , E. , and P. Strike . 1976 . Excision repair of ultraviolet-irradiated deoxyribonucleic acid in plasmnolyzed cells of Escherichia coli . J. Bacteriol. 125 : 787 - 795 .","journal-title":"J. Bacteriol."},{"key":"p_29","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1073\/pnas.51.2.226","article-title":"The disappearance of thymine dimers from DNA: an error-correcting mechanism","volume":"51","author":"Setlow R. B.","year":"1964","unstructured":"Setlow , R. B. , and W. L Carrier . 1964 . The disappearance of thymine dimers from DNA: an error-correcting mechanism . Proc. Natl. Acad. Sci. U.S.A. 51 : 226 - 231 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_30","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1128\/jb.93.3.811-815.1967","article-title":"Fate of thymine-containing dimers in the deoxyribonucleic acid of ultraviolet-irradiated Bacillus subtilis","volume":"93","author":"Shuster R. C.","year":"1967","unstructured":"Shuster , R. C. 1967 . Fate of thymine-containing dimers in the deoxyribonucleic acid of ultraviolet-irradiated Bacillus subtilis . J. Bacteriol. 93 : 811 - 815 .","journal-title":"J. Bacteriol."},{"key":"p_31","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1073\/pnas.56.3.932","article-title":"Repair of DNA studied with a nuclease specific for UV-induced lesions","volume":"56","author":"Strauss B.","year":"1966","unstructured":"Strauss , B. , T. Searashi , and M. Robbins . 1966 . Repair of DNA studied with a nuclease specific for UV-induced lesions . Proc. Natl. Acad. Sci. U.S.A. 56 : 932 - 939 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_32","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0079-6603(08)60647-9","article-title":"Molecular mechanisms of radiation effects","volume":"1","author":"Wacker A.","year":"1963","unstructured":"Wacker , A. 1963 . Molecular mechanisms of radiation effects . Prog. Nucleic Acid Res. Mol. Biol. 1 : 369 - 399 .","journal-title":"Prog. Nucleic Acid Res. Mol. Biol."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.139.1.239-246.1979","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.139.1.239-246.1979","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:34:08Z","timestamp":1627580048000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.139.1.239-246.1979"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,7]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1979,7]]}},"alternative-id":["10.1128\/jb.139.1.239-246.1979"],"URL":"https:\/\/doi.org\/10.1128\/jb.139.1.239-246.1979","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,7]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}