{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T10:48:26Z","timestamp":1770979706074,"version":"3.50.1"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1983,5,1]],"date-time":"1983-05-01T00:00:00Z","timestamp":420595200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1983,5]]},"abstract":"<jats:p>The restriction map of a 46-kilobase fragment of the Rhodopseudomonas capsulata chromosome was aligned with the genetic map of the photosynthesis region of that chromosome by a marker rescue technique. Marker rescue was effected by mobilization of vectors bearing fragments of R. capsulata DNA from Escherichia coli to a set of R. capsulata mutants. Plasmids pDPT51 and pDPT55 were constructed to mediate the intergeneric mobilization of pBR322 derivatives, and a mutant of R. capsulata with improved intergeneric recipient activity was isolated. Four previously unmapped genes affecting bacteriochlorophyll synthesis and two genes affecting photochemical reaction center synthesis have been located by marker rescue. Some of the fragments of R. capsulata DNA are capable of vector-independent complementation, implying that promoters are located on these fragments. Other fragments complement only in one orientation of insertion in the vector, implying transcription from promotors on the vectors and thereby fixing the direction of transcription for those fragments. Still other fragments of DNA show rescue only via recombination between homologous plasmid-borne DNA fragments and chromosomal mutations. The physical dimensions of the genetic map are 3.0 megadaltons per map unit, which agrees with previous estimates based on the size of the R. capsulata gene transfer agent.<\/jats:p>","DOI":"10.1128\/jb.154.2.580-590.1983","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T17:42:44Z","timestamp":1578073364000},"page":"580-590","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":213,"title":["Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique"],"prefix":"10.1128","volume":"154","author":[{"given":"D P","family":"Taylor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S N","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W G","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B L","family":"Marrs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1126\/science.322277","article-title":"Gene cloning and containment properties of plasmid ColEl and its derivatives","volume":"196","author":"Armstrong K. A.","year":"1979","unstructured":"Armstrong , K. A. , V. Hershfield , and D. R. Helinski . 1979 . Gene cloning and containment properties of plasmid ColEl and its derivatives . Science 196 : 172 - 174 .","journal-title":"Science"},{"key":"p_2","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/0147-619X(80)90074-8","article-title":"A plasmid cloning vector for KpnIcleaved DNA","volume":"4","author":"Beckingham K.","year":"1980","unstructured":"Beckingham , K. 1980 . A plasmid cloning vector for KpnIcleaved DNA . Plasmid 4 : 354 - 356 .","journal-title":"Plasmid"},{"key":"p_3","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/0022-2836(69)90288-5","article-title":"A complementation analysis of the restriction and modification of DNA in Escherichia coli","volume":"41","author":"Boyer H. B.","year":"1969","unstructured":"Boyer , H. B. , and D. Roulland-Dussoix . 1969 . A complementation analysis of the restriction and modification of DNA in Escherichia coli . J. Mol. Biol. 41 : 459 - 472 .","journal-title":"J. Mol. Biol."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0022-2836(72)90265-3","article-title":"Protein components of bacterial photosynthetic membranes","volume":"68","author":"Clayton R. K.","year":"1972","unstructured":"Clayton , R. K. , and R. Haselkorn . 1972 . Protein components of bacterial photosynthetic membranes . J. Mol. Biol. 68 : 97 - 105 .","journal-title":"J. Mol. Biol."},{"key":"p_5","first-page":"3240","article-title":"Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA","volume":"70","author":"Cohen S. N.","year":"1972","unstructured":"Cohen , S. N. , A. C. Y. Chang , and L. Hsu . 1972 . Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA . Proc. Natl. Acad. Sci. U.S.A. 70 : 3240 - 3244 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"7347","DOI":"10.1073\/pnas.77.12.7347","article-title":"Broad host range DNA cloning system for Gramnegative bacteria: construction of a gene bank of Rhizobium meliloti","volume":"77","author":"Ditta G.","year":"1980","unstructured":"Ditta , G. , S. Stanfield , D. Corbin , and D. R. Helinski . 1980 . Broad host range DNA cloning system for Gramnegative bacteria: construction of a gene bank of Rhizobium meliloti . Proc. Natl. Acad. Sci. U.S.A. 77 : 7347 - 7351 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-2911(08)60325-2","article-title":"Organization and differentiation of membranes of phototrophic bacteria","volume":"22","author":"Drews G.","year":"1981","unstructured":"Drews , G. , and J. Oelze . 1981 . Organization and differentiation of membranes of phototrophic bacteria . Adv. Microb. Physiol. 22 : 1 - 92 .","journal-title":"Adv. Microb. Physiol."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0378-1119(81)90079-2","article-title":"Specific-purpose plasmid cloning vectors. I. Low copy number, temperature sensitive, mobilization defective pSC101-derived containment vectors","volume":"16","author":"Hashimoto-Gotoh T.","year":"1981","unstructured":"Hashimoto-Gotoh , T. , F. C. H. Franklin , A. Nordheim , and K. N. Timmis . 1981 . Specific-purpose plasmid cloning vectors. I. Low copy number, temperature sensitive, mobilization defective pSC101-derived containment vectors . Gene 16 : 227 - 235 .","journal-title":"Gene"},{"key":"p_9","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/0092-8674(81)90287-7","article-title":"Incompatibility properties of ColEl and pMB1 derivative plasmids: random replication of multicopy replicons","volume":"23","author":"Hashimoto-Gotoh T.","year":"1981","unstructured":"Hashimoto-Gotoh , T. , and K. N. Timnmis . 1981 . Incompatibility properties of ColEl and pMB1 derivative plasmids: random replication of multicopy replicons . Cell 23 : 229 - 238 .","journal-title":"Cell"},{"key":"p_10","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/0092-8674(79)90228-9","article-title":"DNA sequence analysis of the transposon Tn 3: three genes and three sites involved in transposition of Tn 3","volume":"18","author":"Heffron F.","year":"1979","unstructured":"Heffron , F. , B. J. McCarthy , H. Ohtsubo , and E. Ohtsubo . 1979 . DNA sequence analysis of the transposon Tn 3: three genes and three sites involved in transposition of Tn 3 . Cell 18 : 1153 - 1163 .","journal-title":"Cell"},{"key":"p_11","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1128\/jb.146.3.1003-1012.1981","article-title":"Mobilization of the genes for photosynthesis from Rhodopseudomonas capsulata by a promiscuous plasmid","volume":"146","author":"Marrs B. L.","year":"1981","unstructured":"Marrs , B. L. 1981 . Mobilization of the genes for photosynthesis from Rhodopseudomonas capsulata by a promiscuous plasmid . J. Bacteriol. 146 : 1003 - 1012 .","journal-title":"J. Bacteriol."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1128\/jb.143.3.1362-1373.1980","article-title":"Genetic organization of the broad-host-range IncP-1 plasmid R751","volume":"143","author":"Meyer R. J.","year":"1980","unstructured":"Meyer , R. J. , and J. A. Shapiro . 1980 . Genetic organization of the broad-host-range IncP-1 plasmid R751 . J. Bacteriol. 143 : 1362 - 1373 .","journal-title":"J. Bacteriol."},{"key":"p_13","unstructured":"Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratories Cold Spring Harbor N.Y."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"3262","DOI":"10.1093\/nar\/6.10.3267","article-title":"Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313","volume":"6","author":"Rodriguez R. L.","year":"1979","unstructured":"Rodriguez , R. L. , W. R. West , H. L. Heyneker , F. Bolivar , and H. W. Boyer . 1979 . Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313 . Nucleic Acids Res. 6 : 3262 - 3287 .","journal-title":"Nucleic Acids Res."},{"key":"p_15","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1016\/S0021-9258(19)85909-4","article-title":"Biosynthesis of carotenoids derived from neurosporene in Rhodopseudomonas capsulata","volume":"255","author":"Scolrnk P. A.","year":"1980","unstructured":"Scolrnk , P. A. , M. A. Walker , and B. L. Marrs . 1980 . Biosynthesis of carotenoids derived from neurosporene in Rhodopseudomonas capsulata . J. Biol. Chem. 255 : 2427 - 2432 .","journal-title":"J. Biol. Chem."},{"key":"p_16","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/0003-9861(77)90508-2","article-title":"The gene transfer agent of Rhodopseudomonas capsulata. Purification and characterization of its nucleic acid","volume":"181","author":"Solioz M.","year":"1977","unstructured":"Solioz , M. , and B. Marrs . 1977 . The gene transfer agent of Rhodopseudomonas capsulata. Purification and characterization of its nucleic acid . Arch. Biochem. Biophys. 181 : 300 - 307 .","journal-title":"Arch. Biochem. Biophys."},{"key":"p_17","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0022-2836(75)80083-0","article-title":"Detection of specific sequences among DNA fragments separated by agarose gel electrophoresis","volume":"98","author":"Southern E. M.","year":"1975","unstructured":"Southern , E. M. 1975 . Detection of specific sequences among DNA fragments separated by agarose gel electrophoresis . J. Mol. Biol. 98 : 503 - 517 .","journal-title":"J. Mol. Biol."},{"key":"p_18","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1073\/pnas.78.1.167","article-title":"Organization of transcriptional signals in plasmids pBR322 and pACYC184","volume":"78","author":"Stuber D.","year":"1981","unstructured":"Stuber , D. , and H. Bujard . 1981 . Organization of transcriptional signals in plasmids pBR322 and pACYC184 . Proc. Natl. Acad. Sci. U.S.A. 78 : 167 - 171 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1073\/pnas.70.3.799","article-title":"Coupling between bacteriochlorophyll and membrane protein synthesis in Rhodopseudomonas sphaeroides","volume":"70","author":"Takemoto J.","year":"1973","unstructured":"Takemoto , J. , and J. Lascelles . 1973 . Coupling between bacteriochlorophyll and membrane protein synthesis in Rhodopseudomonas sphaeroides . Proc. Natl. Acad. Sci. U.S.A. 70 : 799 - 803 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_20","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1128\/JB.137.1.92-104.1979","article-title":"Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1","volume":"137","author":"Taylor D. P.","year":"1979","unstructured":"Taylor , D. P. , and S. N. Cohen . 1979 . Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1 . J. Bacteriol. 137 : 92 - 104 .","journal-title":"J. Bacteriol."},{"key":"p_21","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1126\/science.325648","article-title":"Rat insulin genes: construction of plasmids containing the coding sequences","volume":"196","author":"Ullrich A.","year":"1977","unstructured":"Ullrich , A. , J. Shine , J. Chirgwin , R. Pictet , E. Tischer , W. J. Rutter , and H. M. Goodman . 1977 . Rat insulin genes: construction of plasmids containing the coding sequences . Science 196 : 1313 - 1318 .","journal-title":"Science"},{"key":"p_22","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/BF00268585","article-title":"ColEl plasmid mobility: essential and conditional functions","volume":"170","author":"Warren G.","year":"1979","unstructured":"Warren , G. , M. W. Saul , and D. J. Sherratt . 1979 . ColEl plasmid mobility: essential and conditional functions . Mol. Gen. Genet. 170 : 103 - 107 .","journal-title":"Mol. Gen. Genet."},{"key":"p_23","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0022-2836(79)90071-8","article-title":"Characterization of the gene transfer agent made by an overproducer mutant of Rhodopseudomonas capsulata","volume":"131","author":"Yen H.","year":"1979","unstructured":"Yen , H. - C. , N. T. Ho , and B. L. Mam . 1979 . Characterization of the gene transfer agent made by an overproducer mutant of Rhodopseudomonas capsulata . J. Mol. Biol. 131 : 157 - 168 .","journal-title":"J. Mol. Biol."},{"key":"p_24","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1128\/JB.126.2.619-629.1976","article-title":"Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata","volume":"126","author":"Yen H.","year":"1976","unstructured":"Yen , H. - C. , and B. Marrs . 1976 . Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata . J. Bacteriol. 126 : 619 - 629 .","journal-title":"J. Bacteriol."},{"key":"p_25","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/0005-2728(81)90214-0","article-title":"Redox chain and energy transduction in chromatophores from Rhodopseudomonas capsulata ceils grown anaerobically in the dark on glucose and dimethyl sulfoxide","volume":"637","author":"Zannol D.","year":"1981","unstructured":"Zannol , D. , and B. L. Marm . 1981 . Redox chain and energy transduction in chromatophores from Rhodopseudomonas capsulata ceils grown anaerobically in the dark on glucose and dimethyl sulfoxide . Biochim. Biophys. Acta 637 : 96 - 106 .","journal-title":"Biochim. Biophys. Acta"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.154.2.580-590.1983","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.154.2.580-590.1983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:31:28Z","timestamp":1627579888000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.154.2.580-590.1983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,5]]},"references-count":25,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1983,5]]}},"alternative-id":["10.1128\/jb.154.2.580-590.1983"],"URL":"https:\/\/doi.org\/10.1128\/jb.154.2.580-590.1983","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,5]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}