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Restriction endonuclease mapping of these HindIII fragments indicated that two of these repeated sequences, identified as IS1-like, occur in reverse orientation adjacent to the ends of the aerobactin iron uptake region, also included in the 16.3-kilobase HindIII fragment. There are two distinct replication regions enclosing the IS1-flanked aerobactin genes. A pColV-K30 BamHI restriction endonuclease fragment, carrying one of these replication regions, showed homology with the F plasmid EcoRI fragment f5, which carries the F replication sequences.<\/jats:p>","DOI":"10.1128\/jb.160.1.256-265.1984","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T18:01:09Z","timestamp":1578074469000},"page":"256-265","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Aerobactin iron uptake sequences in plasmid ColV-K30 are flanked by inverted IS1-like elements and replication regions"],"prefix":"10.1128","volume":"160","author":[{"given":"J F","family":"Perez-Casal","sequence":"first","affiliation":[]},{"given":"J H","family":"Crosa","sequence":"additional","affiliation":[]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1093\/genetics\/39.4.440","article-title":"Segregation of new Iysogenic types during growth of a doubly Iysogenic strain derived from Escherichia coli K12","volume":"39","author":"Appleyard R. 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