{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:41:34Z","timestamp":1761709294389},"reference-count":35,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1990,7,1]],"date-time":"1990-07-01T00:00:00Z","timestamp":646790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1990,7]]},"abstract":"<jats:p>An essential virulence attribute for Yersinia enterocolitica and Yersinia pseudotuberculosis is the ability to invade the intestinal epithelium of mammals. The chromosomal invasin gene (inv) has been cloned from both of these Yersinia species, and the Y. pseudotuberculosis invasin has been well characterized (R. R. Isberg, D. L. Voorhis, and S. Falkow, Cell 50:769-778, 1987). Here we constructed TnphoA translational fusions to the Y. enterocolitica inv gene to identify, characterize, and localize the inv protein product in Escherichia coli. The cloned Y. enterocolitica inv locus encoded a unique protein of ca. 92 kilodaltons when expressed in minicells. A protein of comparable size was detected in immunoblots by using monoclonal antibodies directed against the Y. pseudotuberculosis invasin. This protein, which we also refer to as invasin, promoted both attachment to and invasion of cultured epithelial cells. These two functions were not genetically separable by insertional mutagenesis. We determined that the Y. enterocolitica invasin was localized on the outer membrane and that it was exposed on the bacterial cell surface, which may have implications for how invasin functions to mediate invasion.<\/jats:p>","DOI":"10.1128\/jb.172.7.3780-3789.1990","type":"journal-article","created":{"date-parts":[[2016,11,8]],"date-time":"2016-11-08T14:27:48Z","timestamp":1478615268000},"page":"3780-3789","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["The Yersinia enterocolitica inv gene product is an outer membrane protein that shares epitopes with Yersinia pseudotuberculosis invasin"],"prefix":"10.1128","volume":"172","author":[{"given":"J C","family":"Pepe","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Letters and Science, University of California, Los Angeles 90024."}]},{"given":"V L","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Letters and Science, University of California, Los Angeles 90024."}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1093\/nar\/7.6.1513","article-title":"A rapid alkaline extraction procedure for screening recombinant plasmid DNA","volume":"7","author":"Birnboim H. C.","year":"1979","unstructured":"Birnboim , H. C. , and J. Doly . 1979 . A rapid alkaline extraction procedure for screening recombinant plasmid DNA . Nucleic Acids Res. 7 : 1513 - 1523 .","journal-title":"Nucleic Acids Res."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0003-2697(84)90320-8","article-title":"A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots","volume":"136","author":"Blake M. S.","year":"1984","unstructured":"Blake , M. S. , K. H. Johnston , G. J. Russell-Jones , and E. C. Gotschlich . 1984 . A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots . Anal. Biochem. 136 : 175 - 179 .","journal-title":"Anal. Biochem."},{"key":"p_3","doi-asserted-by":"crossref","unstructured":"Bulla L. A. Jr. and T. C. Cheng (ed.). 1976. Comparative pathobiology vol. 1: Biology of the microsporidia. Plenum Publishing Corp. New York.","DOI":"10.1007\/978-1-4613-4205-2"},{"key":"p_4","doi-asserted-by":"crossref","first-page":"8898","DOI":"10.1073\/pnas.83.23.8898","article-title":"Oxidation of reduced menaquinone by the fumarate reductase complex in Escherichia coli requires the hydrophobic FrdD peptide","volume":"83","author":"Cecchini G.","year":"1986","unstructured":"Cecchini , G. , C. R. Thompson , B. A. C. Ackreli , D. J. Westenberg , N. Dean , and R. P. Gunsalus . 1986 . Oxidation of reduced menaquinone by the fumarate reductase complex in Escherichia coli requires the hydrophobic FrdD peptide . Proc. Natl. Acad. Sci. USA 83 : 8898 - 8902 .","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"p_5","doi-asserted-by":"crossref","first-page":"5173","DOI":"10.1073\/pnas.86.13.5173","article-title":"Penetration of human intestinal epithelial cells by Salmonella: molecular cloning and expression of Salmonella typhi invasion determinants in Escherichia coli","volume":"86","author":"Elsinghorst E. A.","year":"1989","unstructured":"Elsinghorst , E. A. , L. S. Baron , and D. J. Kopecko . 1989 . Penetration of human intestinal epithelial cells by Salmonella: molecular cloning and expression of Salmonella typhi invasion determinants in Escherichia coli . Proc. Natl. Acad. Sci. USA 86 : 5173 - 5177 .","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"p_6","first-page":"227","article-title":"A comparison of microbial invasion strategies of Salmonella, Shigella, and Yersinia species","volume":"64","author":"Finlay B. B.","year":"1987","unstructured":"Finlay , B. B. , and S. Falkow . 1987 . A comparison of microbial invasion strategies of Salmonella, Shigella, and Yersinia species . UCLA Symp. Mol. Cell. Biol. 64 : 227 - 243 .","journal-title":"UCLA Symp. Mol. Cell. Biol."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1111\/j.1365-2958.1988.tb00087.x","article-title":"Identification and characterization of TnphoA mutants of Salmonella that are unable to pass through a polarized MDCK epithelial cell monolayer","volume":"2","author":"FinIay B. B.","year":"1988","unstructured":"FinIay , B. B. , M. N. Starmbach , C. L. Francis , B. A. D. Stocker , S. Chatfield , G. Dougan , and S. Falkow . 1988 . Identification and characterization of TnphoA mutants of Salmonella that are unable to pass through a polarized MDCK epithelial cell monolayer . Mol. Microbiol. 2 : 757 - 766 .","journal-title":"Mol. Microbiol."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-642-50112-8_1","article-title":"Production, properties and utility of bacterial minicells","volume":"69","author":"Frazer A. C.","year":"1975","unstructured":"Frazer , A. C. , and R. Curtiss m. 1975 . 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Isolation, characterization, and mapping of Tn 5 insertions into the 140-megadalton invasion plasmid defective in the mouse Sereny test in Shigella flexneri 2a . Infect. Immun. 54 : 32 - 36 .","journal-title":"Infect. Immun."},{"key":"p_30","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/jb.108.1.545-552.1971","article-title":"Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100","volume":"108","author":"Schnaitman C. A.","year":"1971","unstructured":"Schnaitman , C. A. 1971 . Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100 . J. Bacteriol. 108 : 545 - 552 .","journal-title":"J. Bacteriol."},{"key":"p_31","unstructured":"Silhavy T. J. M. L. Berman and L. W. Enquist. 1984. Experiments with gene fusions. 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Immun."},{"key":"p_35","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1128\/jb.171.3.1616-1622.1989","article-title":"A protein required for transcriptional regulation of Agrobacterium virulence genes spans the cytoplasmtic membrane","volume":"171","author":"Winans S. C.","year":"1989","unstructured":"Winans , S. C. , R. A. Kerstetter , J. E. Ward , and E. W. Nester . 1989 . A protein required for transcriptional regulation of Agrobacterium virulence genes spans the cytoplasmtic membrane . J. Bacteriol. 171 : 1616 - 1622 .","journal-title":"J. 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