{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T03:03:59Z","timestamp":1761102239595},"reference-count":30,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1993,3,1]],"date-time":"1993-03-01T00:00:00Z","timestamp":730944000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1993,3]]},"abstract":"<jats:p>Four Escherichia coli operons, the leuV operon which encodes tRNA(1Leu), the leuX operon which encodes tRNA(6Leu), the metT operon which encodes tRNA(3Leu), and the argT operon which encodes tRNA(1Leu), were examined for the stringent response induced by serine hydroxamate and for growth rate-dependent regulation. In nuclease protection assays, the leuV operon displayed the stringent response in response to leucine starvation, analog inhibition, and growth of a temperature-sensitive leucyl-tRNA synthetase mutant at nonpermissive temperatures. The leuV operon also exhibited the stringent response in multicopy plasmids. The promoters of all four leucyl operons were fused to the gene for beta-galactosidase and inserted into the chromosome by using bacteriophage lambda. All except the leuX promoter displayed growth rate-dependent regulation, consistent with the recent report that the concentration of tRNA(6Leu) actually decreases as growth rate increases. The leuV promoter fused to the beta-galactosidase gene showed a decrease in efficiency in the presence of extrachromosomal copies of rRNA genes. All chromosomal tRNA genes examined showed decreased transcriptional activity following a stringent response, but the leuX gene responded to a lesser extent (3-fold versus 10-fold or more) than the others. Primer extension analysis of this promoter showed little if any response to serine hydroxamate treatment, suggesting that multiple levels of control may exist or that promoter context effects are important in regulation.<\/jats:p>","DOI":"10.1128\/jb.175.5.1309-1315.1993","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T15:42:47Z","timestamp":1478533367000},"page":"1309-1315","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["In vivo regulatory responses of four Escherichia coli operons which encode leucyl-tRNAs"],"prefix":"10.1128","volume":"175","author":[{"given":"K B","family":"Rowley","sequence":"first","affiliation":[{"name":"Department of Microbiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0678."}]},{"given":"R M","family":"Elford","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0678."}]},{"given":"I","family":"Roberts","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0678."}]},{"given":"W M","family":"Holmes","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0678."}]}],"member":"235","reference":[{"key":"p_1","unstructured":"American Type Culture Collection. 1984. 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