{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T11:05:12Z","timestamp":1776510312578,"version":"3.51.2"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[1993,5,1]],"date-time":"1993-05-01T00:00:00Z","timestamp":736214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1993,5]]},"abstract":"<jats:p>Lipid-linked intermediates are involved in the synthesis of the exopolysaccharide xanthan produced by the bacterium Xanthomonas campestris (L. Ielpi, R. O. Couso, and M. A. Dankert, FEBS Lett. 130:253-256, 1981). In this study, the stepwise assembly of the repeating pentasaccharide unit of xanthan is described. EDTA-treated X. campestris cells were used as both enzyme preparation and lipid-P acceptor, and UDP-Glc, GDP-Man, and UDP-glucuronic acid were used as sugar donors. A linear pentasaccharide unit is assembled on a polyprenol-P lipid carrier by the sequential addition of glucose-1-P, glucose, mannose, glucuronic acid, and mannose. The in vitro synthesis of pentasaccharide-P-P-polyprenol was also accompanied by the incorporation of radioactivity into a polymeric product, which was characterized as xanthan, on the basis of gel filtration and permethylation studies. Results from two-stage reactions showed that essentially pentasaccharide-P-P-polyprenol is polymerized. In addition, the direction of chain elongation has been studied by in vivo experiments. The polymerization of lipid-linked repeat units occurs by the successive transfer of the growing chain to a new pentasaccharide-P-P-polyprenol. The reaction involves C-1 of glucose at the reducing end of the polyprenol-linked growing chain and C-4 of glucose at the nonreducing position of the newly formed polyprenol-linked pentasaccharide, generating a branched polymer with a trisaccharide side chain.<\/jats:p>","DOI":"10.1128\/jb.175.9.2490-2500.1993","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T14:44:14Z","timestamp":1478529854000},"page":"2490-2500","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":117,"title":["Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris"],"prefix":"10.1128","volume":"175","author":[{"given":"L","family":"Ielpi","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Bioqu\u00edmicas Fundaci\u00f3n Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R O","family":"Couso","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Bioqu\u00edmicas Fundaci\u00f3n Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M A","family":"Dankert","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Bioqu\u00edmicas Fundaci\u00f3n Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1099\/00221287-136-8-1501","article-title":"A novel galacturonide from Xanthomonas campestris","volume":"136","author":"Baldessari A.","year":"1990","unstructured":"Baldessari , A. , L. lelpi, and M. 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