{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T06:44:29Z","timestamp":1768977869688,"version":"3.49.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[1993,5,1]],"date-time":"1993-05-01T00:00:00Z","timestamp":736214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1993,5]]},"abstract":"<jats:p>Four different mutations were obtained by selecting for resistance to colicin N and screening for continued production of the OmpF protein of Escherichia coli. Two of them also conferred resistance to colicin A. The substitutions C for R-168 (R168C) and E284K caused the loss of the E21 epitope, while the transition G285D altered the E18, E19, and E20 antigenic sites. The substitution G119D drastically affected the stability of the trimeric conformation.<\/jats:p>","DOI":"10.1128\/jb.175.9.2754-2757.1993","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T14:44:14Z","timestamp":1478529854000},"page":"2754-2757","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Specific regions of Escherichia coli OmpF protein involved in antigenic and colicin receptor sites and in stable trimerization"],"prefix":"10.1128","volume":"175","author":[{"given":"D","family":"Fourel","sequence":"first","affiliation":[{"name":"UPR 9027, GDR 1000, Centre National de la Recherche Scientifique, Marseille, France."}]},{"given":"S","family":"Mizushima","sequence":"additional","affiliation":[{"name":"UPR 9027, GDR 1000, Centre National de la Recherche Scientifique, Marseille, France."}]},{"given":"A","family":"Bernadac","sequence":"additional","affiliation":[{"name":"UPR 9027, GDR 1000, Centre National de la Recherche Scientifique, Marseille, France."}]},{"given":"J M","family":"Pag\u00e8s","sequence":"additional","affiliation":[{"name":"UPR 9027, GDR 1000, Centre National de la Recherche Scientifique, Marseille, France."}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/0079-6107(87)90010-1","article-title":"Genetics and biochemistry of the assembly of proteins into the outer membrane of E. coli","volume":"49","author":"Baker K.","year":"1987","unstructured":"Baker , K. , N. Mackman , and I. B. Holland . 1987 . Genetics and biochemistry of the assembly of proteins into the outer membrane of E. coli . Prog. Biophys. Mol. Biol. 49 : 89 - 115 .","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/0022-2836(88)90121-0","article-title":"Mutations that alter the pore function of the OmpF porin of E. coli K-12","volume":"203","author":"Benson S. A.","year":"1988","unstructured":"Benson , S. A. , J. L. L. Occi , and B. A. Sampson . 1988 . Mutations that alter the pore function of the OmpF porin of E. coli K-12 . J. Mol. Biol. 203 : 961 - 970 .","journal-title":"J. Mol. Biol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1432-1033.1988.tb14245.x","article-title":"Permeation of hydrophilic molecules through the outer membrane of gram-negative bacteria: review on bacterial porins","volume":"176","author":"Benz R.","year":"1988","unstructured":"Benz , R. , and K. Bauer . 1988 . Permeation of hydrophilic molecules through the outer membrane of gram-negative bacteria: review on bacterial porins . Eur. J. Biochem. 176 : 1 - 19 .","journal-title":"Eur. J. Biochem."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/0300-9084(90)90062-L","article-title":"A genetic engineering approach to study the mode of assembly of the OmpF porin in the envelope of E. coli","volume":"72","author":"Bolla J.","year":"1990","unstructured":"Bolla , J. - M. , A. Bernadac , C. Lazdunski , and J. -M. Pages . 1990 . A genetic engineering approach to study the mode of assembly of the OmpF porin in the envelope of E. coli . Biochimie 72 : 385 - 395 .","journal-title":"Biochimie"},{"key":"p_5","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1007\/BF00332243","article-title":"The role of the carboxy-terminal membrane spanning fragment in the biogenesis of E. coli outer membrane protein PhoE","volume":"216","author":"Bosch D.","year":"1989","unstructured":"Bosch , D. , M. Scholten , C. Verhagen , and J. Tommassen . 1989 . The role of the carboxy-terminal membrane spanning fragment in the biogenesis of E. coli outer membrane protein PhoE . Mol. Gen. Genet. 216 : 144 - 148 .","journal-title":"Mol. Gen. Genet."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/358727a0","article-title":"Crystal structures explain functional properties of two E. coli porins","volume":"358","author":"Cowan S. W.","year":"1992","unstructured":"Cowan , S. W. , T. Schirmer , G. Rummel , M. Steiert , R. Ghosh , R. A. Pauptit , J. N. Jansonius , and J. P. Rosenbusch . 1992 . Crystal structures explain functional properties of two E. coli porins . Nature (London) 358 : 727 - 733 .","journal-title":"Nature (London)"},{"key":"p_7","doi-asserted-by":"crossref","first-page":"10217","DOI":"10.1016\/S0021-9258(18)86933-2","article-title":"In vitro folding and oligomerization of a membrane protein. Transition of bacterial porin from random coil to native conformation","volume":"265","author":"Eisele J.","year":"1990","unstructured":"Eisele , J. - L. , and J. P. Rosenbusch . 1990 . In vitro folding and oligomerization of a membrane protein. Transition of bacterial porin from random coil to native conformation . J. Biol. Chem. 265 : 10217 - 10220 .","journal-title":"J. Biol. Chem."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"3675","DOI":"10.1128\/JB.172.7.3675-3680.1990","article-title":"Characterization of OmpF domains involved in E. coli K-12 sensitivity to colicins A and N","volume":"172","author":"Fourel D.","year":"1990","unstructured":"Fourel , D. , S. Hikita , J. -M. Bolla , S. Mizushima , and J. -M. Pagis . 1990 . Characterization of OmpF domains involved in E. coli K-12 sensitivity to colicins A and N . J. Bacteriol. 172 : 3675 - 3680 .","journal-title":"J. Bacteriol."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1111\/j.1432-1033.1992.tb16907.x","article-title":"Dynamics of the exposure of epitopes on OmpF, an outer membrane protein of E. coli","volume":"206","author":"Fourel D.","year":"1992","unstructured":"Fourel , D. , S. Mizushima , and J. -M. Pages . 1992 . Dynamics of the exposure of epitopes on OmpF, an outer membrane protein of E. coli . Eur. J. Biochem. 206 : 109 - 114 .","journal-title":"Eur. J. Biochem."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1128\/JB.169.3.929-933.1987","article-title":"Role of porins in outer membrane permeability","volume":"169","author":"Hancock R. E. W.","year":"1987","unstructured":"Hancock , R. E. W. 1987 . Role of porins in outer membrane permeability . J. Bacteriol. 169 : 929 - 933 .","journal-title":"J. Bacteriol."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0923-2508(89)90074-0","article-title":"Structural and functional characterization of the OmpF and OmpC porins of the E. coli outer membrane","volume":"140","author":"Hikita C.","year":"1989","unstructured":"Hikita , C. , Y. Satake , H. Yamada , T. Mizuno , and S. Mizushima . 1989 . Structural and functional characterization of the OmpF and OmpC porins of the E. coli outer membrane . Res. Microbiol. 140 : 177 - 190 .","journal-title":"Res. Microbiol."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"6957","DOI":"10.1093\/nar\/10.21.6957","article-title":"Primary structure of the ompF gene that codes for a major outer membrane protein of E. coli K-12","volume":"10","author":"Inokuchi K.","year":"1982","unstructured":"Inokuchi , K. , N. Mutoh , S. Matsuyama , and S. Mizushima . 1982 . Primary structure of the ompF gene that codes for a major outer membrane protein of E. coli K-12 . Nucleic Acids Res. 10 : 6957 - 6968 .","journal-title":"Nucleic Acids Res."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"2377","DOI":"10.1002\/j.1460-2075.1985.tb03942.x","article-title":"An inner membrane protein N-terminal signal sequence is able to promote efficient localization of an outer membrane protein in E. coli","volume":"4","author":"Jackson M. E.","year":"1985","unstructured":"Jackson , M. E. , J. M. Pratt , N. G. Stoker , and I. B. Holland . 1985 . An inner membrane protein N-terminal signal sequence is able to promote efficient localization of an outer membrane protein in E. coli . EMBO J. 4 : 2377 - 2383 .","journal-title":"EMBO J."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1111\/j.1365-2958.1991.tb02145.x","article-title":"The bacterial porin superfamily: sequence alignment and structure prediction","volume":"5","author":"Jeanteur D.","year":"1991","unstructured":"Jeanteur , D. , J. H. Lakey , and F. Pattus . 1991 . The bacterial porin superfamily: sequence alignment and structure prediction . Mol. Microbiol. 5 : 2153 - 2164 .","journal-title":"Mol. Microbiol."},{"key":"p_15","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0014-5793(91)80076-F","article-title":"ompC mutants which allow growth on maltodextrins show increased channel size and greater voltage sensitivity","volume":"278","author":"Lakey J. H.","year":"1991","unstructured":"Lakey , J. H. , E. J. A. Lea , and F. Pattus . 1991 . ompC mutants which allow growth on maltodextrins show increased channel size and greater voltage sensitivity . FEBS Lett. 278 : 31 - 34 .","journal-title":"FEBS Lett."},{"key":"p_16","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0304-4157(83)90014-X","article-title":"Molecular architecture and functioning of the outer membrane of E. coli and other gram-negative bacteria","volume":"737","author":"Lugtenberg B.","year":"1983","unstructured":"Lugtenberg , B. , and L. van Alphen . 1983 . Molecular architecture and functioning of the outer membrane of E. coli and other gram-negative bacteria . Biochim. Biophys. Acta 737 : 51 - 115 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_17","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/0161-5890(89)90067-9","article-title":"Immunological analysis of porin polymorphism in E. coli B and K-12","volume":"26","author":"Lupi N.","year":"1989","unstructured":"Lupi , N. , A. Bourgois , A. Bernadac , S. Laboucarie , and J. -M. Pages . 1989 . Immunological analysis of porin polymorphism in E. coli B and K-12 . Mol. Immunol. 26 : 1027 - 1036 .","journal-title":"Mol. Immunol."},{"key":"p_18","doi-asserted-by":"crossref","first-page":"3611","DOI":"10.1128\/JB.170.8.3611-3617.1988","article-title":"Genetic identification of the pore domain of the OmpC porin of E. coli","volume":"170","author":"Misra R.","year":"1988","unstructured":"Misra , R. , and S. A. Benson . 1988 . Genetic identification of the pore domain of the OmpC porin of E. coli . J. Bacteriol. 170 : 3611 - 3617 .","journal-title":"J. Bacteriol."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/BF00331581","article-title":"Construction of a series of ompC-ompF chimeric genes by in vivo homologous recombination in E. coli and characterization of their translational products","volume":"207","author":"Mizuno T.","year":"1987","unstructured":"Mizuno , T. , H. Kasai , and S. Mizushima . 1987 . Construction of a series of ompC-ompF chimeric genes by in vivo homologous recombination in E. coli and characterization of their translational products . Mol. Gen. Genet. 207 : 217 - 223 .","journal-title":"Mol. Gen. Genet."},{"key":"p_20","doi-asserted-by":"crossref","unstructured":"Nakamura K. and S. Mizushima. 1976. Effects of heating in dodecyl sulfate solution on the conformation and electrophoretic mobility of isolated major outer membrane proteins NOTES 2757","DOI":"10.1093\/oxfordjournals.jbchem.a131414"},{"key":"p_21","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1111\/j.1365-2958.1992.tb01487.x","article-title":"Porins and specific channels of bacterial outer membranes","volume":"6","author":"Nikaido H.","year":"1992","unstructured":"Nikaido , H. 1992 . Porins and specific channels of bacterial outer membranes . Mol. Microbiol. 6 : 435 - 442 .","journal-title":"Mol. Microbiol."},{"key":"p_22","unstructured":"Nikaido H. and M. Vaara. 1987. Outer membrane p. 7-22. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology vol. 1. American Society for Microbiology Washington D.C."},{"key":"p_23","first-page":"168","article-title":"The ins and outs of colicins. Part I: production, and translocation across membrane","volume":"1","author":"Pugsley A. P.","year":"1984","unstructured":"Pugsley , A. P. 1984 . The ins and outs of colicins. Part I: production, and translocation across membrane . Microbiol. Sci. 1 : 168 - 176 .","journal-title":"Microbiol. Sci."},{"key":"p_24","doi-asserted-by":"crossref","first-page":"7753","DOI":"10.1016\/S0021-9258(18)68563-1","article-title":"Targeting of porin to the outer membrane of E. coli","volume":"263","author":"Reid J.","year":"1988","unstructured":"Reid , J. , H. Fung , K. Gehring , P. Klebba , and H. Nikaido . 1988 . Targeting of porin to the outer membrane of E. coli . J. Biol. Chem. 263 : 7753 - 7759 .","journal-title":"J. Biol. Chem."},{"key":"p_25","doi-asserted-by":"crossref","first-page":"8019","DOI":"10.1016\/S0021-9258(19)42066-8","article-title":"Characterization of the major envelope protein from E. coli","volume":"249","author":"Rosenbusch J. P.","year":"1974","unstructured":"Rosenbusch , J. P. 1974 . Characterization of the major envelope protein from E. coli . J. Biol. Chem. 249 : 8019 - 8029 .","journal-title":"J. Biol. Chem."},{"key":"p_26","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1128\/mr.53.3.333-366.1989","article-title":"Insertion of proteins into bacterial membranes: mechanism, characteristics, and comparisons with the eucaryotic process","volume":"53","author":"Saier M. H.","year":"1989","unstructured":"Saier , M. H. , P. K. Werner , and M. Muller . 1989 . Insertion of proteins into bacterial membranes: mechanism, characteristics, and comparisons with the eucaryotic process . Microbiol. Rev. 53 : 333 - 366 .","journal-title":"Microbiol. Rev."},{"key":"p_27","doi-asserted-by":"crossref","first-page":"5463","DOI":"10.1073\/pnas.74.12.5463","article-title":"DNA sequencing with chain-terminating inhibitors","volume":"74","author":"Sanger F.","year":"1977","unstructured":"Sanger , F. , S. Nicklen , and A. R. Coulson . 1977 . DNA sequencing with chain-terminating inhibitors . Proc. Natl. Acad. Sci. USA 74 : 5463 - 5467 .","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"p_28","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0022-2836(91)90880-F","article-title":"Carboxyterminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein","volume":"218","author":"Struyvi M.","year":"1991","unstructured":"Struyvi , M. , M. Moons , and J. Tommassen . 1991 . Carboxyterminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein . J. Mol. Biol. 218 : 141 - 148 .","journal-title":"J. Mol. Biol."},{"key":"p_29","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1002\/j.1460-2075.1983.tb01581.x","article-title":"The ultimate localization of an outer membrane protein of E. coli K-12 is not determined by the signal sequence","volume":"2","author":"Tommassen J.","year":"1983","unstructured":"Tommassen , J. , H. van Tol , and B. Lugtenberg . 1983 . The ultimate localization of an outer membrane protein of E. coli K-12 is not determined by the signal sequence . EMBO J. 2 : 1275 - 1279 .","journal-title":"EMBO J."},{"key":"p_30","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1093\/oxfordjournals.jbchem.a122076","article-title":"Use of a series of OmpFOmpC chimeric proteins for locating antigenic determinants recognized by monoclonal antibodies against the OmpC and OmpF proteins of the E. coli outer membrane","volume":"102","author":"Yamada H.","year":"1987","unstructured":"Yamada , H. , N. Oshima , T. Mizuno , H. Matsui , Y. Kai , H. Noguchi , and S. Mizushima . 1987 . Use of a series of OmpFOmpC chimeric proteins for locating antigenic determinants recognized by monoclonal antibodies against the OmpC and OmpF proteins of the E. coli outer membrane . J. Biochem. 102 : 455 - 464 .","journal-title":"J. Biochem."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.175.9.2754-2757.1993","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.175.9.2754-2757.1993","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T17:02:49Z","timestamp":1657645369000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.175.9.2754-2757.1993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,5]]},"references-count":30,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1993,5]]}},"alternative-id":["10.1128\/jb.175.9.2754-2757.1993"],"URL":"https:\/\/doi.org\/10.1128\/jb.175.9.2754-2757.1993","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,5]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}