{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:16:09Z","timestamp":1762866969131},"reference-count":28,"publisher":"American Society for Microbiology","issue":"15","license":[{"start":{"date-parts":[[1997,8,1]],"date-time":"1997-08-01T00:00:00Z","timestamp":870393600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1997,8]]},"abstract":"<jats:p>We have previously reported the nucleotide sequence of the Streptococcus pneumoniae type 19F capsular polysaccharide synthesis locus (cps19f), which consists of 15 open reading frames (ORFs) designated cps19fA to -O. Hybridization analysis indicated that close homologs for cps19fA to -H and cps19fK to -O were found in type 19B, but there were no homologs for cps19fI and -J. In this study we used long-range PCR to amplify and clone a 10.5-kb section of the S. pneumoniae type 19B capsule locus (cps19b) between cps19bH and cps19bK. This region of the cps19b locus is 4 kb larger than that in the cps19f locus and replaces cps19fI and cps19fJ with five new ORFs, designated cps19bP, -I, -Q, -R, and -J. We have proposed functions for four of the protein products, including functional homologs of Cps19fI and Cps19fJ. Transformation of a S. pneumoniae mutant containing an interrupted type 19F capsule locus with the 10.5-kb cps19b PCR product converted the recipient strain to type 19B. Southern hybridization analysis indicated that cps19bP, -I, -Q, -R, and -J are unique to type 19B and the closely related type 19C.<\/jats:p>","DOI":"10.1128\/jb.179.15.4953-4958.1997","type":"journal-article","created":{"date-parts":[[2016,11,9]],"date-time":"2016-11-09T13:19:36Z","timestamp":1478697576000},"page":"4953-4958","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Molecular and genetic characterization of the capsule biosynthesis locus of Streptococcus pneumoniae type 19B"],"prefix":"10.1128","volume":"179","author":[{"given":"J K","family":"Morona","sequence":"first","affiliation":[{"name":"Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, South Australia."}]},{"given":"R","family":"Morona","sequence":"additional","affiliation":[{"name":"Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, South Australia."}]},{"given":"J C","family":"Paton","sequence":"additional","affiliation":[{"name":"Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, South Australia."}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul S. 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