{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T09:12:41Z","timestamp":1775293961512,"version":"3.50.1"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[1998,6,1]],"date-time":"1998-06-01T00:00:00Z","timestamp":896659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Escherichia coli<\/jats:italic>\n            WP2 bacteria with an ochre amino acid auxotrophy show no evidence of growth during the first few days after plating at densities above 10\n            <jats:sup>8<\/jats:sup>\n            on plates lacking the required amino acid. They lose viability for some days, and then a subpopulation recovers and there is cell turnover. At very low plating densities (around 10\n            <jats:sup>2<\/jats:sup>\n            per plate), almost every cell will eventually form a small but visible colony. At intermediate plating densities (10\n            <jats:sup>6<\/jats:sup>\n            to 10\n            <jats:sup>7<\/jats:sup>\n            per plate), there is an immediate increase in the number of viable bacteria. The results are consistent with a model that assumes that growth is dependent on trace amounts of tryptophan or a tryptophan-complementing substance and that death is due to extracellular toxic species in the medium, including active oxygen species. Mutations in\n            <jats:italic>mutT<\/jats:italic>\n            bacteria under these conditions result from incorporation of 7,8-dihydro-8-oxo-dGTP into DNA and thus largely reflect DNA synthesis associated with the increase in the number of viable cells at the initial density used (10\n            <jats:sup>7<\/jats:sup>\n            per plate). We show that the increase in cell number and much of this DNA synthesis can be eliminated by the presence of 10\n            <jats:sup>8<\/jats:sup>\n            scavenger bacteria and by removal of early-arising mutant colonies that release the required amino acid. The synthesis that remains is equivalent to less than a quarter of a genome per day and is marginally reduced, if at all, in a\n            <jats:italic>polA<\/jats:italic>\n            derivative. We cannot exclude the possibility that this residual DNA synthesis is peculiar to\n            <jats:italic>mutT<\/jats:italic>\n            bacteria due to transcriptional leakiness, although there is no evidence that this is a major problem in this strain. If such DNA synthesis also occurs in wild-type bacteria, it may well be important for adaptive mutation since use of a more refined agar in selective plates both eliminated the initial increase in cell number seen at low density (10\n            <jats:sup>7<\/jats:sup>\n            per plate) and reduced the rate of appearance of mutants at plating densities above 10\n            <jats:sup>8<\/jats:sup>\n            per plate.\n          <\/jats:p>","DOI":"10.1128\/jb.180.11.2906-2910.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T16:23:38Z","timestamp":1577809418000},"page":"2906-2910","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["DNA Synthesis and Viability of a\n            <i>mutT<\/i>\n            Derivative of\n            <i>Escherichia coli<\/i>\n            WP2 under Conditions of Amino Acid Starvation and Relation to Stationary-Phase (Adaptive) Mutation"],"prefix":"10.1128","volume":"180","author":[{"given":"Bryn A.","family":"Bridges","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->MRC Cell Mutation Unit, University of Sussex, Brighton, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon","family":"Ereira","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->MRC Cell Mutation Unit, University of Sussex, Brighton, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/0027-5107(69)90060-8","article-title":"Radiation sensitive mutants of T4D. 1. 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