{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:35:29Z","timestamp":1777462529611,"version":"3.51.4"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[1998,7,1]],"date-time":"1998-07-01T00:00:00Z","timestamp":899251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1998,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The effect of CheY and fumarate on switching frequency and rotational bias of the bacterial flagellar motor was analyzed by computer-aided tracking of tethered\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . Plots of cells overexpressing CheY in a gutted background showed a bell-shaped correlation curve of switching frequency and bias centering at about 50% clockwise rotation. Gutted cells (i.e., with\n            <jats:italic>cheA<\/jats:italic>\n            to\n            <jats:italic>cheZ<\/jats:italic>\n            deleted) with a low CheY level but a high cytoplasmic fumarate concentration displayed the same correlation of switching frequency and bias as cells overexpressing CheY at the wild-type fumarate level. Hence, a high fumarate level can phenotypically mimic CheY overexpression by simultaneously changing the switching frequency and the bias. A linear correlation of cytoplasmic fumarate concentration and clockwise rotation bias was found and predicts exclusively counterclockwise rotation without switching when fumarate is absent. This suggests that (i) fumarate is essential for clockwise rotation in vivo and (ii) any metabolically induced fluctuation of its cytoplasmic concentration will result in a transient change in bias and switching probability. A high fumarate level resulted in a dose-response curve linking bias and cytoplasmic CheY concentration that was offset but with a slope similar to that for a low fumarate level. It is concluded that fumarate and CheY act additively presumably at different reaction steps in the conformational transition of the switch complex from counterclockwise to clockwise motor rotation.\n          <\/jats:p>","DOI":"10.1128\/jb.180.13.3375-3380.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T16:24:27Z","timestamp":1577809467000},"page":"3375-3380","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Regulation of Switching Frequency and Bias of the Bacterial Flagellar Motor by CheY and Fumarate"],"prefix":"10.1128","volume":"180","author":[{"given":"Marco","family":"Montrone","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Max-Planck-Institut fu\u0308r Biochemie, 82152 Martinsried, Germany,1 and"}]},{"given":"Michael","family":"Eisenbach","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Biological Chemistry, The Weizmann Institute of Science, 76100 Rehovot, Israel2"}]},{"given":"Dieter","family":"Oesterhelt","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Max-Planck-Institut fu\u0308r Biochemie, 82152 Martinsried, Germany,1 and"}]},{"given":"Wolfgang","family":"Marwan","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Max-Planck-Institut fu\u0308r Biochemie, 82152 Martinsried, Germany,1 and"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1021\/bi00121a034","article-title":"Correlation between phosphorylation of the chemotaxis protein CheY and its activity at the flagellar motor","volume":"31","author":"Barak R.","year":"1992","unstructured":"Barak R. 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