{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T04:36:37Z","timestamp":1774326997479,"version":"3.50.1"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[1998,11,1]],"date-time":"1998-11-01T00:00:00Z","timestamp":909878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1998,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>Streptococcus salivarius<\/jats:italic>\n            57.I\n            <jats:italic>ure<\/jats:italic>\n            cluster was organized as an operon, beginning with\n            <jats:italic>ureI<\/jats:italic>\n            , followed by\n            <jats:italic>ureABC<\/jats:italic>\n            (structural genes) and\n            <jats:italic>ureEFGD<\/jats:italic>\n            (accessory genes). Northern analyses revealed transcripts encompassing structural genes and transcripts containing the entire operon. A \u03c2\n            <jats:sup>70<\/jats:sup>\n            -like promoter could be mapped 5\u2032 to\n            <jats:italic>ureI<\/jats:italic>\n            (\n            <jats:italic>PureI<\/jats:italic>\n            ) by primer extension analysis. The intensity of the signal increased when cells were grown at an acidic pH and was further enhanced by excess carbohydrate. To determine the function(s) of two inverted repeats located 5\u2032 to\n            <jats:italic>PureI<\/jats:italic>\n            , transcriptional fusions of the full-length promoter region (\n            <jats:italic>PureI<\/jats:italic>\n            ), or a deletion derivative (\n            <jats:italic>PureI<\/jats:italic>\n            \u0394100), and a promoterless chloramphenicol acetyltransferase (CAT) gene were constructed and integrated into the chromosome to generate strains\n            <jats:italic>PureI<\/jats:italic>\n            CAT and\n            <jats:italic>PureI<\/jats:italic>\n            \u0394100CAT, respectively. CAT specific activities of\n            <jats:italic>PureI<\/jats:italic>\n            CAT were repressed at pH 7.0 and induced at pH 5.5 and by excess carbohydrate. In\n            <jats:italic>PureI<\/jats:italic>\n            \u0394100CAT, CAT activity was 60-fold higher than in\n            <jats:italic>PureI<\/jats:italic>\n            CAT at pH 7.0 and pH induction was nearly eliminated, indicating that expression was negatively regulated. Thus, it was concluded that\n            <jats:italic>PureI<\/jats:italic>\n            was the predominant, regulated promoter and that regulation was governed by a mechanism differing markedly from other known mechanisms for bacterial urease expression.\n          <\/jats:p>","DOI":"10.1128\/jb.180.21.5769-5775.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T16:28:51Z","timestamp":1577809731000},"page":"5769-5775","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":90,"title":["Transcriptional Regulation of the\n            <i>Streptococcus salivarius<\/i>\n            57.I Urease Operon"],"prefix":"10.1128","volume":"180","author":[{"given":"Yi-Ywan M.","family":"Chen","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Center for Oral Biology1 and"},{"name":"<!--label omitted: 2-->Department of Microbiology and Immunology,2 School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642"}]},{"given":"Cheryl A.","family":"Weaver","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Oral Biology1 and"}]},{"given":"David R.","family":"Mendelsohn","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Oral Biology1 and"}]},{"given":"Robert A.","family":"Burne","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Oral Biology1 and"},{"name":"<!--label omitted: 2-->Department of Microbiology and Immunology,2 School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Ausubel\nF. 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