{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T06:55:09Z","timestamp":1761893709467},"reference-count":38,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The complete sequencing and annotation of the 181.7-kb\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            virulence plasmid pXO1 predicted 143 genes but could only assign putative functions to 45. Hybridization assays, PCR amplification, and DNA sequencing were used to determine whether pXO1 open reading frame (ORF) sequences were present in other bacilli and more distantly related bacterial genera. Eighteen\n            <jats:italic>Bacillus<\/jats:italic>\n            species isolates and four other bacterial species were tested for the presence of 106 pXO1 ORFs. Three ORFs were conserved in most of the bacteria tested. Many of the pXO1 ORFs were detected in closely related\n            <jats:italic>Bacillus<\/jats:italic>\n            species, and some were detected only in\n            <jats:italic>B. anthracis<\/jats:italic>\n            isolates. Three isolates,\n            <jats:italic>Bacillus cereus<\/jats:italic>\n            D-17,\n            <jats:italic>B. cereus<\/jats:italic>\n            43881, and\n            <jats:italic>Bacillus thuringiensis<\/jats:italic>\n            33679, contained sequences that were similar to more than one-half of the pXO1 ORF sequences examined. The majority of the DNA fragments that were amplified by PCR from these organisms had DNA sequences between 80 and 98% similar to that of pXO1. Pulsed-field gel electrophoresis revealed large potential plasmids present in both\n            <jats:italic>B. cereus<\/jats:italic>\n            43881 (341 kb) and\n            <jats:italic>B. thuringiensis<\/jats:italic>\n            ATCC 33679 (327 kb) that hybridized with a DNA probe composed of six pXO1 ORFs.\n          <\/jats:p>","DOI":"10.1128\/jb.184.1.134-141.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:09Z","timestamp":1027764069000},"page":"134-141","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":103,"title":["<i>Bacillus anthracis<\/i>\n            pXO1 Plasmid Sequence Conservation among Closely Related Bacterial Species"],"prefix":"10.1128","volume":"184","author":[{"given":"James","family":"Pannucci","sequence":"first","affiliation":[{"name":"Biosciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard T.","family":"Okinaka","sequence":"additional","affiliation":[{"name":"Biosciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Sabin","sequence":"additional","affiliation":[{"name":"Biosciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl R.","family":"Kuske","sequence":"additional","affiliation":[{"name":"Biosciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1099\/13500872-141-4-983","volume":"141","year":"1995","unstructured":"Agata, N., M. 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