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Approximately 1,500 to 2,000 SMC molecules are present per cell during active growth, corresponding to one SMC complex per 6,000 to 8,000 bp of chromosomal DNA. Although transcription from the<jats:italic>smc<\/jats:italic>promoter is induced during early S phase, a cell cycle transcription pattern previously observed with multiple DNA replication and repair genes, the SMC protein is present throughout the entire cell cycle. Examination of the intracellular location of SMC showed that in swarmer cells, which do not replicate DNA, the protein forms two or three foci. Stalked cells, which are actively engaged in DNA replication, have three or four SMC foci per cell. The SMC foci appear randomly distributed in the cell. Many predivisional cells have bright polar SMC foci, which are lost upon cell division. Thus, chromosome compaction likely involves dynamic aggregates of SMC bound to DNA. The aggregation pattern changes as a function of the cell cycle both during and upon completion of chromosome replication.<\/jats:p>","DOI":"10.1128\/jb.185.10.3068-3075.2003","type":"journal-article","created":{"date-parts":[[2003,5,2]],"date-time":"2003-05-02T16:23:48Z","timestamp":1051892628000},"page":"3068-3075","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Cell-Cycle-Regulated Expression and Subcellular Localization of the<i>Caulobacter crescentus<\/i>SMC Chromosome Structural Protein"],"prefix":"10.1128","volume":"185","author":[{"given":"Rasmus B.","family":"Jensen","sequence":"first","affiliation":[{"name":"Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, California 94305-5329"}]},{"given":"Lucy","family":"Shapiro","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, California 94305-5329"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"24088","DOI":"10.1074\/jbc.273.37.24088","volume":"273","year":"1998","unstructured":"Akhmedov, A. 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