{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T16:56:30Z","timestamp":1700758590291},"reference-count":24,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1962,11,1]],"date-time":"1962-11-01T00:00:00Z","timestamp":-226195200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1962,11]]},"abstract":"<jats:p>\n            <jats:sc>Suit, Joan<\/jats:sc>\n            C. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston). Ribonucleic acid in a \u201cmembrane\u201d fraction of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and its relation to cell-wall synthesis. J. Bacteriol.\n            <jats:bold>84:<\/jats:bold>\n            1061\u20131070. 1962.\u2014A small amount of ribonucleic acid (RNA) was found in a \u201cmembrane\u201d fraction prepared from osmotically sensitized\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . It exhibited an elevated metabolic activity in that it attained the highest specific activity of any RNA in subcellular fractions of logarithmic-phase cells or spheroplasts prepared from logarithmic-phase cells which had been allowed to incorporate P\n            <jats:sup>32<\/jats:sup>\n            briefly. The metabolic activity of this RNA, in terms of P\n            <jats:sup>32<\/jats:sup>\n            incorporation, was found to be independent of cell-wall synthesis in the diaminopimelic acid (DAP)-less mutant,\n            <jats:italic>E. coli<\/jats:italic>\n            W 173-25, but was inhibited by penicillin in both this strain and in\n            <jats:italic>E. coli<\/jats:italic>\n            B. The latter effect is considered to be a result of other complex inhibitions of cellular metabolism by the antibiotic. The development of sensitivity to osmotic shock, capability of recovery, and synthesis of macromolecules in penicillin-treated and DAP-starved cultures, under these conditions, is described.\n          <\/jats:p>","DOI":"10.1128\/jb.84.5.1061-1070.1962","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T21:23:59Z","timestamp":1578086639000},"page":"1061-1070","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["RIBONUCLEIC ACID IN A \u201cMEMBRANE\u201d FRACTION OF\n            <i>ESCHERICHIA COLI<\/i>\n            AND ITS RELATION TO CELL-WALL SYNTHESIS"],"prefix":"10.1128","volume":"84","author":[{"given":"Joan C.","family":"Suit","sequence":"first","affiliation":[{"name":"Section of Genetics, Department of Biology, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/S0021-9258(18)81381-3","article-title":"Polynucleotide phosphorylase in isolated bacterial cell membranes","volume":"237","author":"ABRAMS A.","year":"1962","unstructured":"ABRAMS , A. , AND P. Mc NAMARA. 1962 . Polynucleotide phosphorylase in isolated bacterial cell membranes . J. Biol. Chem. 237 : 170 - 175 .","journal-title":"J. Biol. Chem."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"3659","DOI":"10.1016\/S0021-9258(18)64525-9","article-title":"Adenosine triphosphate in isolated bacteria] cell membranes","volume":"235","author":"ABRAMS A., P.","year":"1960","unstructured":"ABRAMS , A., P. MCNAMARA , A ND F . B. JOHNSON. 1960 . Adenosine triphosphate in isolated bacteria] cell membranes . J. Biol. Chem. 235 : 3659 - 3662 .","journal-title":"J. Biol. Chem."},{"key":"p_3","unstructured":"ADAMS M. H. 1959. Bacteriophages. Interscience Publishers Inc. New York p. 450-451."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1042\/bj0730396","article-title":"Site of synthesis of the peptide component of the cell wall of Bacillus megaterium","volume":"73","author":"BROOKES P., A.","year":"1959","unstructured":"BROOKES , P., A. R. CRATHORN , A ND G . D. HUNTER. 1959 . Site of synthesis of the peptide component of the cell wall of Bacillus megaterium . Biochem. J. 73 : 396 - 401 .","journal-title":"Biochem. J."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1128\/JB.78.1.41-48.1959","article-title":"Nucleic acid metabolism and ribonucleic acid heterogeneity in Escherichia coli","volume":"78","author":"COUNTRYMAN J. L.","year":"1959","unstructured":"COUNTRYMAN , J. L. , AND E. VOLKIN. 1959 . Nucleic acid metabolism and ribonucleic acid heterogeneity in Escherichia coli . J. Bacteriol. 78 : 41 - 48 .","journal-title":"J. Bacteriol."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1038\/169534a0","article-title":"Biosynthetic interrelationships of Iysine, diaminopimelic acid, and threonine in mutants of Escherichia coli","volume":"169","author":"DAVIS B. D.","year":"1952","unstructured":"DAVIS , B. D. 1952 . Biosynthetic interrelationships of Iysine, diaminopimelic acid, and threonine in mutants of Escherichia coli . Nature 169 : 534 - 536 .","journal-title":"Nature"},{"key":"p_7","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1042\/bj0810059","article-title":"Cellular components of Bacillus megaterium and their role in protein biosynthesis","volume":"81","author":"GODSON G. N.","year":"1961","unstructured":"GODSON , G. N. , G. D. HUNTER , A ND J . A. V. BUTLER . 1961 . Cellular components of Bacillus megaterium and their role in protein biosynthesis . Biochem. J. 81 : 59 - 65 .","journal-title":"Biochem. J."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0006-3002(61)90790-9","article-title":"Effect of sonic oscillation upon \"old\" and ''new\" nucleic acids in Escherichia coli","volume":"53","author":"GOLDSTEIN A.","year":"1961","unstructured":"GOLDSTEIN , A. , AND B. J. BROWN. 1961 . Effect of sonic oscillation upon \"old\" and ''new\" nucleic acids in Escherichia coli . Biochim. et Biophys. Acta 53 : 19 - 28 .","journal-title":"Biochim. et Biophys. Acta"},{"key":"p_9","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/190581a0","article-title":"Unstable ribonucleic acid revealed by pulse labeling of Escherichia coli","volume":"190","author":"GROS F., H.","year":"1961","unstructured":"GROS , F., H. HIATT , W. GILBERT , C. G. KURLAND , R. W. RISEBROUGH , A ND J . D. WATSON. 1961 . Unstable ribonucleic acid revealed by pulse labeling of Escherichia coli . Nature 190 : 581 - 585 .","journal-title":"Nature"},{"key":"p_10","first-page":"436440","article-title":"Quantitative aspects of penicillin action on Escherichia coli in hypertonic medium","volume":"80","author":"HUGO W. B.","year":"1960","unstructured":"HUGO , W. B. , AND A. D. RUSSELL. 1960 . Quantitative aspects of penicillin action on Escherichia coli in hypertonic medium . J. Bacteriol. 80 : 436440 .","journal-title":"J. Bacteriol."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1042\/bj0730369","article-title":"Intermediate reactions in protein synthesis by the isolated cytoplasmic-membrane fraction of Bacillus megaterium","volume":"73","author":"HUNTER G. D.","year":"1959","unstructured":"HUNTER , G. D. , P. BROOKES , A. R. CRATHORN , A ND J . A. V. BUTLER . 1959 . Intermediate reactions in protein synthesis by the isolated cytoplasmic-membrane fraction of Bacillus megaterium . Biochem. J. 73 : 369 - 376 .","journal-title":"Biochem. J."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1128\/jb.76.6.612-617.1958","article-title":"Studies in the physiology and biochemistry of penicillin-induced spheroplasts of Escherichia coli","volume":"76","author":"HURWITZ C., J.","year":"1958","unstructured":"HURWITZ , C., J. M. REINER , A ND J . V. LANDAU . 1958 . Studies in the physiology and biochemistry of penicillin-induced spheroplasts of Escherichia coli . J. Bacteriol. 76 : 612 - 617 .","journal-title":"J. Bacteriol."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1128\/JB.79.5.697-706.1960","article-title":"Lysis of frozen and thawed cells of Escherichia coli by Iysozyme, and their conversion into spheroplasts","volume":"79","author":"KOHN A.","year":"1960","unstructured":"KOHN , A. 1960 . Lysis of frozen and thawed cells of Escherichia coli by Iysozyme, and their conversion into spheroplasts . J. Bacteriol. 79 : 697 - 706 .","journal-title":"J. Bacteriol."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1128\/JB.81.6.875-886.1961","article-title":"Genetic nature of stable L forms of Salmonella paratyphi","volume":"81","author":"LANDMAN E.","year":"1961","unstructured":"LANDMAN , 0. E. , AND H. S. GINOZA. 1961 . Genetic nature of stable L forms of Salmonella paratyphi . J. Bacteriol. 81 : 875 - 886 .","journal-title":"J. Bacteriol."},{"key":"p_15","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1073\/pnas.42.9.574","article-title":"Bacterial protoplasts induced by penicillin","author":"LEDERBERG J.","year":"1956","unstructured":"LEDERBERG , J. 1956 . Bacterial protoplasts induced by penicillin . Proc. Natl. Acad. Sci. U. S. 42: 574 - 577 .","journal-title":"Proc. Natl. Acad. Sci. U."},{"key":"p_16","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1128\/jb.75.2.143-160.1958","article-title":"Protoplasts and L-type growth of Escherichia coli","volume":"75","author":"LEDERBERG J.","year":"1958","unstructured":"LEDERBERG , J. , AND J. ST. CLAIR . 1958 . Protoplasts and L-type growth of Escherichia coli . J. Bacteriol. 75 : 143 - 160 .","journal-title":"J. Bacteriol."},{"key":"p_17","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"LOWRY H., N.","year":"1951","unstructured":"LOWRY , 0. H., N. J. ROSEBROUGH , A. L. FARR , A ND R . J. RANDALL. 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 .","journal-title":"J. Biol. Chem."},{"key":"p_18","first-page":"410","article-title":"Bacterial \"protoplasts","volume":"27","author":"MCQUILLEN K.","year":"1958","unstructured":"MCQUILLEN , K. 1958 . Bacterial \"protoplasts \": effects of diaminopimelic acid deprival and penicillin addition compared in Escherichia coli. Biochim. et Biophys. Acta 27 : 410 - 411 .","journal-title":"Biochim. et Biophys. Acta"},{"key":"p_19","unstructured":"MCQUILLEN K. 1960. Bacterial protoplasts p. 249-359. In I. C. Gunsalus and R. Y. Stanier [ed.] The bacteria. Academic Press Inc. New York."},{"key":"p_20","doi-asserted-by":"crossref","unstructured":"PANGBORN J. A. G. MARR AND S. A. ROBRISH. 1962. Location of respiratory enzymes in the intracytoplasmic membranes of Azotobacter agilis. Bacteriol. Proc. p. 122.","DOI":"10.1128\/JB.84.4.669-678.1962"},{"key":"p_21","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1128\/JB.74.1.48-59.1957","article-title":"Induction of bacterial Iysis by penicillin","volume":"74","author":"PRESTIDGE L. S.","year":"1957","unstructured":"PRESTIDGE , L. S. , AND A. B. PARDEE. 1957 . Induction of bacterial Iysis by penicillin . J. Bacteriol. 74 : 48 - 59 .","journal-title":"J. Bacteriol."},{"key":"p_22","unstructured":"SPIEGELMAN S. 1959. Protein and nucleic acid synthesis in subcellular fractions of bacterial cells p. 81-103. In Recent progress in microbiology Symposium VIIth International Congress for Microbiology Stockholm 1958 Charles C Thomas Springfield Ill."},{"key":"p_23","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/0042-6822(56)90001-0","article-title":"Intracellular distribution of labeled ribonucleic acid after phage infection of Escherichia coli","volume":"2","author":"VOLKIN E.","year":"1956","unstructured":"VOLKIN , E. , AND L. ASTRACHAN. 1956 a. Intracellular distribution of labeled ribonucleic acid after phage infection of Escherichia coli . Virology 2 : 433 - 437 .","journal-title":"Virology"},{"key":"p_24","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0042-6822(56)90016-2","article-title":"Phosphorus incorporation in Escherichia coli ribonucleic acid after infection with bacteriophage T2","volume":"2","author":"VOLKIN E.","year":"1956","unstructured":"VOLKIN , E. , AND L. ASTRACHAN. 1956 b. Phosphorus incorporation in Escherichia coli ribonucleic acid after infection with bacteriophage T2 . Virology 2 : 149 - 161 .","journal-title":"Virology"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.84.5.1061-1070.1962","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.84.5.1061-1070.1962","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:11:42Z","timestamp":1627578702000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.84.5.1061-1070.1962"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1962,11]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1962,11]]}},"alternative-id":["10.1128\/jb.84.5.1061-1070.1962"],"URL":"https:\/\/doi.org\/10.1128\/jb.84.5.1061-1070.1962","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1962,11]]},"assertion":[{"value":"2021-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}