{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T08:14:50Z","timestamp":1779178490842,"version":"3.51.4"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1965,12,1]],"date-time":"1965-12-01T00:00:00Z","timestamp":-128908800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1965,12]]},"abstract":"<jats:p>\n            <jats:sc>Morris<\/jats:sc>\n            , D. W. (University of California, San Diego),\n            <jats:sc>and J. A. DeMoss<\/jats:sc>\n            . Role of aminoacyl-transfer ribonucleic acid in the regulation of ribonucleic acid synthesis in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . J. Bacteriol.\n            <jats:bold>90:<\/jats:bold>\n            1624\u20131631. 1965.\u2014A leucine auxotroph of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            was examined for its rate of ribonucleic acid (RNA) synthesis and the level of charged leucine-, arginine-, and valine-specific transfer RNA (tRNA) during the exponential growth period and when growth was limited by leucine starvation. During the logarithmic growth period, the leucine-specific tRNA was 70% charged, arginine-specific tRNA was 30% charged, and the valine-specific tRNA was 80% charged. When leucine became limiting, RNA synthesis was inhibited and the levels of charged arginine- and valine-specific tRNA remained constant, whereas the level of charged leucine-specific tRNA dropped to 40%. Examination of the leucyl-tRNA during the leucine starvation period showed that this 40% level is maintained by protein turnover. Addition of chloramphenicol or puromycin to a leucine-starved culture derepressed RNA synthesis. In the presence of chloramphenicol, the leucine-specific tRNA was fully charged; however, in the presence of puromycin the amount of charged leucine-specific tRNA remained at the starved level. Therefore, during leucine starvation the level of uncharged leucine-specific tRNA is not invariably correlated with the rate of RNA synthesis. We propose that it is the availability of charged tRNA and not the amount of uncharged tRNA which is the important factor in the amino acid control of RNA synthesis.\n          <\/jats:p>","DOI":"10.1128\/jb.90.6.1624-1631.1965","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T20:51:06Z","timestamp":1578084666000},"page":"1624-1631","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Role of Aminoacyl-Transfer Ribonucleic Acid in the Regulation of Ribonucleic Acid Synthesis in\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"90","author":[{"given":"D. W.","family":"Morris","sequence":"first","affiliation":[{"name":"Department of Biology, Revelle College, University of California, San Diego, La Jolla, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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