{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T05:50:17Z","timestamp":1776923417709,"version":"3.51.2"},"reference-count":11,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1968,5,1]],"date-time":"1968-05-01T00:00:00Z","timestamp":-52704000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1968,5]]},"abstract":"<jats:p>\n            A methanogenic bacterium, present in bovine rumen contents at concentrations of approximately 2 \u00d7 10\n            <jats:sup>8<\/jats:sup>\n            cells per ml, has been isolated in pure culture. The organism is a strictly anaerobic, weakly motile, nonsporeforming, gram-negative rod (0.7 \u03bc \u00d7 1.5 to 2.0 \u03bc) with rounded ends. There is a single polar flagellum. The organism grows at temperatures between 30 and 45 C, with an optimum at 40 C, and at\n            <jats:italic>p<\/jats:italic>\n            H values between 5.9 and 7.7, with optimal growth between\n            <jats:italic>p<\/jats:italic>\n            H 6.1 and 6.9. Of the 17 substrates tested, only formate and H\n            <jats:sub>2<\/jats:sub>\n            plus CO\n            <jats:sub>2<\/jats:sub>\n            supported growth. An unidentified, heat-stable factor(s) was required by the organism. The factor, which was not one of the common ones, was present in rumen fluid, mixed rumen bacteria, and yeast extract. On the basis of colony morphology, Gram reaction, and motility, the organism is classified as a new species of methanogenic bacterium, and the name\n            <jats:italic>Methanobacterium mobilis<\/jats:italic>\n            sp. n. is proposed.\n          <\/jats:p>","DOI":"10.1128\/jb.95.5.1943-1951.1968","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:08:06Z","timestamp":1578190086000},"page":"1943-1951","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":151,"title":["Characterization of\n            <i>Methanobacterium mobilis<\/i>\n            , sp. n., Isolated from the Bovine Rumen"],"prefix":"10.1128","volume":"95","author":[{"given":"M. J. B.","family":"Paynter","sequence":"first","affiliation":[{"name":"Department of Bacteriology, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. E.","family":"Hungate","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, University of California, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1038\/170576a0","article-title":"Methane fermentation in the rumen of cattle","volume":"170","author":"BEIJER W. H.","year":"1952","unstructured":"BEIJER , W. H. 1952 . Methane fermentation in the rumen of cattle . Nature 170 : 576 - 577 .","journal-title":"Nature"},{"key":"p_2","unstructured":"BRYANT M. P. 1965. Rumen methanogenic bacteria p. 411-418. In R. W. Dougherty [ed.] Physiology of digestion in the reminant. Butterworths London."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1128\/JB.7.2.159-182.1922","article-title":"Notes on the gram stain with description of a new method","volume":"7","author":"BURKE V.","year":"1922","unstructured":"BURKE , V. 1922 . Notes on the gram stain with description of a new method . J. Bacteriol. 7 : 159 - 182 .","journal-title":"J. Bacteriol."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/BR.14.1.1-49.1950","article-title":"The anaerobic mesophilic cellulolytic bacteria","volume":"14","author":"HUNGATE R. E.","year":"1950","unstructured":"HUNGATE , R. E. 1950 . The anaerobic mesophilic cellulolytic bacteria . Bacteriol. Rev. 14 : 1 - 49 .","journal-title":"Bacteriol. Rev."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1128\/BR.24.4.353-364.1960","article-title":"Symposium: selected topics in microbial ecology. I. Microbial ecology of the rumen","volume":"24","author":"HUNGATE R. E.","year":"1960","unstructured":"HUNGATE , R. E. 1960 . Symposium: selected topics in microbial ecology. I. Microbial ecology of the rumen . Bacteriol. Rev. 24 : 353 - 364 .","journal-title":"Bacteriol. Rev."},{"key":"p_6","unstructured":"HUNGATE R. E. 1966. The rumen and its microbes p. 26-30. Academic Press Inc. New York."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1007\/BF00406327","article-title":"Hydrogen as an intermediate in the rumen fermentation","volume":"59","author":"HUNGATE R. E.","year":"1967","unstructured":"HUNGATE , R. E. 1967 . Hydrogen as an intermediate in the rumen fermentation . Arch. Mikrobiol. 59 : 158 - 164 .","journal-title":"Arch. Mikrobiol."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1017\/S0021859600022371","article-title":"A comparison of the rumen fermentation in European and Zebu cattle","volume":"54","author":"HUNGATE R. E.","year":"1960","unstructured":"HUNGATE , R. E. , G. D. PHILLIPS , D. P. HUNGATE , A ND A . MA cGR EGOR . 1960 . A comparison of the rumen fermentation in European and Zebu cattle . J. Agr. Sci. 54 : 196 - 201 .","journal-title":"J. Agr. Sci."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1128\/JB.91.2.908-909.1966","article-title":"Suitability of butyl rubber stoppers for closing anaerobic roll culture tubes","volume":"91","author":"HUNGATE R. E.","year":"1966","unstructured":"HUNGATE , R. E. , W. SMITH , A ND R . T. J. Clarke. 1966 . Suitability of butyl rubber stoppers for closing anaerobic roll culture tubes . J. Bacteriol. 91 : 908 - 909 .","journal-title":"J. Bacteriol."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"779","DOI":"10.3168\/jds.S0022-0302(57)94554-X","article-title":"In vitro studies on methanogenic rumen bacteria","volume":"40","author":"OPPERMAN R. A.","year":"1957","unstructured":"OPPERMAN , R. A. , W. 0. NELSON , A ND R . E. BROWN. 1957 . In vitro studies on methanogenic rumen bacteria . J. Dairy Sci. 40 : 779 - 788 .","journal-title":"J. Dairy Sci."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1128\/JB.75.6.713-718.1958","article-title":"Isolation and characterization of Methanobacterium ruminantium n. sp","volume":"75","author":"SMITH P. H.","year":"1958","unstructured":"SMITH , P. H. , AND R. E. HUNGATE. 1958 . Isolation and characterization of Methanobacterium ruminantium n. sp . J. Bacteriol. 75 : 713 - 718 .","journal-title":"J. Bacteriol."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.95.5.1943-1951.1968","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.95.5.1943-1951.1968","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T16:46:02Z","timestamp":1627577162000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.95.5.1943-1951.1968"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,5]]},"references-count":11,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1968,5]]}},"alternative-id":["10.1128\/jb.95.5.1943-1951.1968"],"URL":"https:\/\/doi.org\/10.1128\/jb.95.5.1943-1951.1968","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1968,5]]},"assertion":[{"value":"2021-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}