{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:45:50Z","timestamp":1773283550008,"version":"3.50.1"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A study was designed to assess the importance of sequence types among extended-spectrum \u03b2-lactamase (ESBL)-producing\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            isolates causing bacteremia over an 11-year period (2000 to 2010) in a centralized Canadian region. A total of 197 patients with incident infections were identified; the majority presented with community-onset urosepsis, with a significant increase in the prevalence of ESBL-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            during the later part of the study. The majority of\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            isolates produced either CTX-M-15 or CTX-M-14. We identified 7 different major sequence types among 91% of isolates (i.e., the ST10 clonal complex, ST38, ST131, ST315, ST393, ST405, and ST648) and provided insight into their clinical and molecular characteristics. ST38 was the most antimicrobial-susceptible sequence type and predominated during 2000 to 2004 but disappeared after 2008. ST131 was the most antimicrobial-resistant sequence type, and the influx of a single pulsotype of this sequence type was responsible for the significant increase of ESBL-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            strains since 2007. During 2010, 49\/63 (78%) of the ESBL-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            isolates belonged to ST131, and this sequence type had established itself as a major drug-resistant pathogen in Calgary, Alberta, Canada, posing an important new public health threat within our region. We urgently need well-designed epidemiological and molecular studies to understand the dynamics of transmission, risk factors, and reservoirs for\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            ST131. This will provide insight into the emergence and spread of this multiresistant sequence type.\n          <\/jats:p>","DOI":"10.1128\/jcm.06025-11","type":"journal-article","created":{"date-parts":[[2011,12,8]],"date-time":"2011-12-08T06:03:05Z","timestamp":1323324185000},"page":"294-299","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":134,"title":["Molecular Epidemiology over an 11-Year Period (2000 to 2010) of Extended-Spectrum \u03b2-Lactamase-Producing Escherichia coli Causing Bacteremia in a Centralized Canadian Region"],"prefix":"10.1128","volume":"50","author":[{"given":"Gisele","family":"Peirano","sequence":"first","affiliation":[{"name":"Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada"},{"name":"Departments of Pathology &amp; Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada"}]},{"given":"Akke K.","family":"van der Bij","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Centre, Rotterdam"},{"name":"Department of Medical Microbiology, Reinier de Graaf Hospital, Delft, the Netherlands"}]},{"given":"Daniel B.","family":"Gregson","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada"},{"name":"Departments of Pathology &amp; Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada"},{"name":"Medicine, University of Calgary, Calgary, Alberta, Canada"}]},{"given":"Johann D. D.","family":"Pitout","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada"},{"name":"Departments of Pathology &amp; Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada"},{"name":"Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr235"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.08.011"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp194"},{"key":"e_1_3_2_6_2","unstructured":"Clinical and Laboratory Standards Institute. 2009. Performance standards for antimicrobial susceptibility testing; 19th informational supplement.\nM100-S21. Clinical and Laboratory Standards Institute Wayne PA."},{"key":"e_1_3_2_7_2","unstructured":"Clinical and Laboratory Standards Institute. 2011. Performance standards for antimicrobial susceptibility testing; 21st informational supplement.\nM100-S21. 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