{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T01:11:23Z","timestamp":1774314683246,"version":"3.50.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1998,6,1]],"date-time":"1998-06-01T00:00:00Z","timestamp":896659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Salmonella typhi<\/jats:italic>\n            was isolated from 369 and\n            <jats:italic>Salmonella paratyphi<\/jats:italic>\n            A was isolated from 6 of 515 Vietnamese patients with suspected enteric fever. Compared with conventional broth culture of blood, direct plating of the buffy coat had a diagnostic sensitivity of 99.5% (95% confidence interval [CI], 97.1 to 100%). Blood bacterial counts were estimated by the pour plate method. The median\n            <jats:italic>S. typhi<\/jats:italic>\n            count in blood was 1 CFU\/ml (range, &lt;0.3 to 387 CFU\/ml), of which a mean of 63% (95% CI, 58 to 67%) were intracellular. The mean number of bacteria per infected leukocyte was 1.3 (interquartile range [IQR], 0.7 to 2.4) CFU\/cell (\n            <jats:italic>n<\/jats:italic>\n            = 81). Children (&lt;15 years old;\n            <jats:italic>n<\/jats:italic>\n            = 115) had higher median blood bacterial counts than adults (\n            <jats:italic>n<\/jats:italic>\n            = 262): 1.5 (range, &lt;0.3 to 387) versus 0.6 (range, &lt;0.3 to 17.7) CFU\/ml (\n            <jats:italic>P<\/jats:italic>\n            = 0.008), and patients who excreted\n            <jats:italic>S. typhi<\/jats:italic>\n            in feces had higher bacteremias than those who did not: a median of 3 (range, &lt;0.3 to 32) versus 1 (range, &lt;0.3 to 68) CFU\/ml (\n            <jats:italic>P<\/jats:italic>\n            = 0.02). Blood bacterial counts declined with increasing duration of illness (\n            <jats:italic>P<\/jats:italic>\n            = 0.002) and were higher in infections caused by multidrug-resistant\n            <jats:italic>S. typhi<\/jats:italic>\n            (1.3 [range, &lt;0.3 to 387] CFU\/ml;\n            <jats:italic>n<\/jats:italic>\n            = 313) than in infections caused by antibiotic-sensitive\n            <jats:italic>S. typhi<\/jats:italic>\n            (0.5 [range, &lt;0.3 to 32] CFU\/ml;\n            <jats:italic>n<\/jats:italic>\n            = 62) (\n            <jats:italic>P<\/jats:italic>\n            = 0.006). In a multivariate analysis this proved to be an independent association, suggesting a relationship between antibiotic resistance and virulence in\n            <jats:italic>S. typhi.<\/jats:italic>\n          <\/jats:p>","DOI":"10.1128\/jcm.36.6.1683-1687.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:32:00Z","timestamp":1577813520000},"page":"1683-1687","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":223,"title":["Quantitation of Bacteria in Blood of Typhoid Fever Patients and Relationship between Counts and Clinical Features, Transmissibility, and Antibiotic Resistance"],"prefix":"10.1128","volume":"36","author":[{"given":"John","family":"Wain","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Wellcome Trust Clinical Research Unit1 and"},{"name":"<!--label omitted: 2-->Department of Microbiology,2 Centre for Tropical Diseases, Cho Quan Hospital, Ho Chi Minh City, and"},{"name":"<!--label omitted: 3-->Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, United Kingdom3"}]},{"given":"To Song","family":"Diep","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology,2 Centre for Tropical Diseases, Cho Quan Hospital, Ho Chi Minh City, and"}]},{"given":"Vo Anh","family":"Ho","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Dong Thap Hospital, Cao Lanh, Dong Thap,4 Vietnam, and"}]},{"given":"Amanda M.","family":"Walsh","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Wellcome Trust Clinical Research Unit1 and"},{"name":"<!--label omitted: 3-->Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, United Kingdom3"}]},{"given":"Nguyen Thi Tuyet","family":"Hoa","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology,2 Centre for Tropical Diseases, Cho Quan Hospital, Ho Chi Minh City, and"}]},{"given":"Christopher M.","family":"Parry","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Wellcome Trust Clinical Research Unit1 and"},{"name":"<!--label omitted: 2-->Department of Microbiology,2 Centre for Tropical Diseases, Cho Quan Hospital, Ho Chi Minh City, and"},{"name":"<!--label omitted: 3-->Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, United Kingdom3"}]},{"given":"Nicholas J.","family":"White","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Wellcome Trust Clinical Research Unit1 and"},{"name":"<!--label omitted: 3-->Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, United Kingdom3"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/45.4_ts.493"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/13.5.832"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1978.03630270047018"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(97)90102-4"},{"key":"e_1_3_2_6_2","first-page":"896","article-title":"The bacteriology of the blood in typhoid fever: an analysis of 1602 cases","volume":"133","author":"Coleman W.","year":"1907","unstructured":"Coleman W. 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Invest."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1955.00250140125014"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/149.2.157"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197009242831306"},{"key":"e_1_3_2_16_2","first-page":"137","article-title":"Factors affecting mortality of patients with bacteremia","volume":"137","author":"Kluge R. M.","year":"1973","unstructured":"Kluge R. M. DuPont H. L. Factors affecting mortality of patients with bacteremia.Surg. Gynecol. Obstet. 137 1973 137 267","journal-title":"Surg. Gynecol. Obstet."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(80)90101-1"},{"key":"e_1_3_2_18_2","unstructured":"Parker\nM. T.\nSalmonella\nTopley and Wilson\u2019s principles of bacteriology virology and immunity\n7th ed.\nWilson\nG.\nMiles\nA.\nParker\nM. 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Unpublished observation."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.6.1498"},{"key":"e_1_3_2_26_2","first-page":"128","article-title":"Isolation of Salmonella typhi from the blood stream","volume":"46","author":"Watson K.","year":"1955","unstructured":"Watson K. Isolation of Salmonella typhi from the blood stream.J. Lab. Clin. Med. 46 1955 128 134","journal-title":"J. Lab. Clin. 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