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Daudi cells were treated under these conditions, and gene expression microarray analyses were performed. The results of the analysis identified 25 genes associated with IFN-\u03b1 AV activity. Upregulation of 23 IFN-induced genes was confirmed by using reverse transcription-PCR. Of 25 gene products, 17 were detected by Western blotting at 24 h. Of the 25 genes, 10 have not been previously linked to AV activity of IFN-\u03b1. The most upregulated gene was IFIT3 (for IFN-induced protein with tetratricopeptide repeats 3). The results from antibody neutralizing experiments suggested an association of the identified genes with IFN-\u03b1 AV activity. This association was strengthened by results from IFIT3-small interfering RNA transfection experiments showing decreased expression of IFIT3 and a reduction in the AV activity induced by IFN-\u03b1. Overexpression of IFIT3 resulted in a decrease of virus titer. Transcription of AV genes after the treatment of cells with higher concentrations of IFN having an AP effect on Daudi cells suggested pleiotropic functions of identified gene products.<\/jats:p>","DOI":"10.1128\/jvi.00818-10","type":"journal-article","created":{"date-parts":[[2010,8,5]],"date-time":"2010-08-05T03:53:47Z","timestamp":1280980427000},"page":"10671-10680","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["Identification of Alpha Interferon-Induced Genes Associated with Antiviral Activity in Daudi Cells and Characterization of IFIT3 as a Novel Antiviral Gene"],"prefix":"10.1128","volume":"84","author":[{"given":"H.","family":"Schmeisser","sequence":"first","affiliation":[{"name":"National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland 20892"}]},{"given":"J.","family":"Mejido","sequence":"additional","affiliation":[{"name":"National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland 20892"}]},{"given":"C. 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