{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T21:47:21Z","timestamp":1770328041590,"version":"3.49.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1977,8,1]],"date-time":"1977-08-01T00:00:00Z","timestamp":239241600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[1977,8]]},"abstract":"<jats:p>\n            The expression of guinea pig retrovirus (5-bromodeoxyuridine[BUdR]-induced GPV) was studied in guinea pig L\n            <jats:sub>2<\/jats:sub>\n            C leukemic lymphoblasts by use of molecular hybridization of viral complementary DNA (cDNA) to cellular RNA. It was found that L\n            <jats:sub>2<\/jats:sub>\n            C leukemic lymphoblasts, leukemic spleen, and BUdR-induced virus-producing cells contain virus-specific RNA: 0.05% (800 to 960 copies per cell), 0.02% (360 copies per cell), and 0.3% (5,120 copies per cell), respectively. Adult normal liver and spleen, on the other hand, contain less than 0.2 copy of viral RNA per cell. Both BUdR-induced cells and L\n            <jats:sub>2<\/jats:sub>\n            C leukemic lymphoblasts contained 14S, 22S, 35S, and 70S RNA species of total and cytoplasmic virus-specific RNA as determined by sucrose velocity gradient analysis and hybridization of sucrose gradient fractions to cDNA. Virus-specific mRNA was identified in both BUdR-induced cells and L\n            <jats:sub>2<\/jats:sub>\n            C leukemic lymphoblasts by the criterion that it cosedimented with purified polyribosomes in a sucrose gradient and that it changed to a lower sedimentation value if polyribosomes were disaggregated with EDTA prior to centrifugation. Virus-specific mRNA obtained from either the polyribosome region of purified polyribosomes or the released messenger region of EDTA-disaggregated purified polyribosomes consisted of 14S, 20S, and 35S species in both BUdR-induced cells and L\n            <jats:sub>2<\/jats:sub>\n            C leukemic lymphoblasts. Hybridization of cDNA to the RNA of L\n            <jats:sub>2<\/jats:sub>\n            C leukemic lymphoblasts and BUdR-induced cells was essentially complete. Additionally, leukemic lymphoblast RNA could displace 95% of the hybridization of BUdR-induced GPV 70S RNA to guinea pig DNA. The midpoints of thermal denaturation of hybrids formed between GPV cDNA and the RNA of either L\n            <jats:sub>2<\/jats:sub>\n            C leukemic lymphoblasts or the 70S RNA of BUdR-induced GPV were both 89\u00b0C in 2\u00d7 concentrated 0.15 M NaCl plus 0.015 M sodium citrate. These results show that BUdR-induced GPV genes are essentially completely expressed in L\n            <jats:sub>2<\/jats:sub>\n            C leukemic lymphoblasts and that virus-specific mRNA is present, although fewer copies of RNA are present in L\n            <jats:sub>2<\/jats:sub>\n            C leukemic lymphoblasts than in BUdR-induced cells.\n          <\/jats:p>","DOI":"10.1128\/jvi.23.2.263-271.1977","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T00:36:24Z","timestamp":1578270984000},"page":"263-271","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Expression of Endogenous Retroviral Genes in Leukemic Guinea Pig Cells"],"prefix":"10.1128","volume":"23","author":[{"given":"A. R.","family":"Davis","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, University of California at Los Angeles, Los Angeles, California 90024"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. P.","family":"Nayak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, University of California at Los Angeles, Los Angeles, California 90024"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.23.2.263-271.1977","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.23.2.263-271.1977","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T22:18:37Z","timestamp":1646432317000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.23.2.263-271.1977"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,8]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1977,8]]}},"alternative-id":["10.1128\/jvi.23.2.263-271.1977"],"URL":"https:\/\/doi.org\/10.1128\/jvi.23.2.263-271.1977","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,8]]},"assertion":[{"value":"1977-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}