{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T15:56:55Z","timestamp":1765382215249},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1979,12,1]],"date-time":"1979-12-01T00:00:00Z","timestamp":312854400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[1979,12]]},"abstract":"<jats:p>A plaque morphology mutant (pm-522) of human papovavirus BK, which was rescued from a human papovavirus BK-induced hamster pineocytoma, was characterized and compared with a cloned wild-type virus (wt-501). Mutant pm-522 formed turbid plaques and grew more slowly than wt-501 in human embryonic kidney (HEK) cells. The immunofluorescence assay revealed that more HEK cells underwent abortive infection with pm-522 than with wt-501. Whereas wt-501 induced brain tumors and osteosarcomas, but no insulinomas, in hamsters, pm-522 induced brain tumors and insulinomas. The DNA of pm-522 was found by electrophoresis and electron microscopy to have a deletion (85 +\/- 15 base pairs) and an insertion (40 +\/- 10 base pairs) between map coordinates 0.708 and 0.725 from the endonuclease EcoRI cleavage site. These results demonstrate the presence of a viable deletion human papovarivus BK mutant capable of inducing insulinomas in hamsters.<\/jats:p>","DOI":"10.1128\/jvi.32.3.934-942.1979","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T00:50:42Z","timestamp":1578271842000},"page":"934-942","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Viable deletion mutant of human papovavirus BK that induces insulinomas in hamsters"],"prefix":"10.1128","volume":"32","author":[{"given":"S","family":"Watanabe","sequence":"first","affiliation":[]},{"given":"K","family":"Yoshiike","sequence":"additional","affiliation":[]},{"given":"A","family":"Nozawa","sequence":"additional","affiliation":[]},{"given":"Y","family":"Yuasa","sequence":"additional","affiliation":[]},{"given":"S","family":"Uchida","sequence":"additional","affiliation":[]}],"member":"235","container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.32.3.934-942.1979","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.32.3.934-942.1979","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T22:29:17Z","timestamp":1646432957000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.32.3.934-942.1979"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,12]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1979,12]]}},"alternative-id":["10.1128\/jvi.32.3.934-942.1979"],"URL":"https:\/\/doi.org\/10.1128\/jvi.32.3.934-942.1979","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,12]]},"assertion":[{"value":"1979-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}