{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T19:17:22Z","timestamp":1770491842357,"version":"3.49.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[1995,10,1]],"date-time":"1995-10-01T00:00:00Z","timestamp":812505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[1995,10]]},"abstract":"<jats:p>Expression of the v-fms oncogene of feline sarcoma virus in fibroblasts causes surface exposure of an activated receptor tyrosine kinase, v-Fms, that is autophosphorylated at multiple sites within its cytoplasmic domain. Cellular proteins interacting with this part of v-Fms modulate the mitogenic activity and morphology of the cells. We show here that the tyrosine residue in position 807 (Y-807) of the v-Fms molecule constitutes a major autophosphorylation site. The replacement of this residue by phenylalanine (Y807F mutation) allowed us to functionally dissect v-Fms-specific mitogenic and morphogenic cascades. Cells expressing the mutant v-Fms molecule resembled wild-type (wt) v-Fms-transformed (wt-v-Fms) cells in terms of [3H]thymidine uptake rates and activation of the Ras\/Raf-1 mitogenic cascade. Such cells showed, however, a flat morphology and contained intact actin cables and fibronectin network. Our studies indicate that the v-Fms molecule controls cell morphology by a cascade that involves a direct interaction with p120RasGAP and p190RhoGAP: (i) in contrast to wt v-Fms molecules, the Y807F v-Fms protein failed to associate with and phosphorylate p120RasGAP; (ii) tight complexes between p120RasGAP and p190RhoGAP as well as detectable RhoGAP activity were present exclusively in wt-v-Fms cells; and (iii) p190RhoGAP was dispersed throughout the cytoplasm of wt-v-Fms cells, whereas its distribution was restricted to perinuclear regions of cells expressing the mutant v-Fms gene.<\/jats:p>","DOI":"10.1128\/jvi.69.10.6010-6020.1995","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T21:21:00Z","timestamp":1578345660000},"page":"6010-6020","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Tyrosine 807 of the v-Fms oncogene product controls cell morphology and association with p120RasGAP"],"prefix":"10.1128","volume":"69","author":[{"given":"S","family":"Trouliaris","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Virologie, Justus-Liebig-Universit\u00e4t Giessen, Germany."}]},{"given":"U","family":"Smola","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Virologie, Justus-Liebig-Universit\u00e4t Giessen, Germany."}]},{"given":"J H","family":"Chang","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Virologie, Justus-Liebig-Universit\u00e4t Giessen, Germany."}]},{"given":"S J","family":"Parsons","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Virologie, Justus-Liebig-Universit\u00e4t Giessen, Germany."}]},{"given":"H","family":"Niemann","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Virologie, Justus-Liebig-Universit\u00e4t Giessen, Germany."}]},{"given":"T","family":"Tamura","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Virologie, Justus-Liebig-Universit\u00e4t Giessen, Germany."}]}],"member":"235","container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.69.10.6010-6020.1995","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.69.10.6010-6020.1995","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T13:09:55Z","timestamp":1646485795000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.69.10.6010-6020.1995"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1995,10]]}},"alternative-id":["10.1128\/jvi.69.10.6010-6020.1995"],"URL":"https:\/\/doi.org\/10.1128\/jvi.69.10.6010-6020.1995","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,10]]},"assertion":[{"value":"1995-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}