{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T18:13:19Z","timestamp":1761156799129},"reference-count":39,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The translation elongation factor 1\u03b4 (EF-1\u03b4) consists of two forms, a hypophosphorylated form (apparent\n            <jats:italic>M<\/jats:italic>\n            <jats:sub>r<\/jats:sub>\n            , 38,000) and a hyperphosphorylated form (apparent\n            <jats:italic>M<\/jats:italic>\n            <jats:sub>r<\/jats:sub>\n            , 40,000). Earlier Y. Kawaguchi, R. Bruni, and B. Roizman (J. Virol. 71:1019\u20131024, 1997) reported that whereas mock-infected cells accumulate the hypophosphorylated form, the hyperphosphorylated form of EF-1\u03b4 accumulates in cells infected with herpes simplex virus 1. We now report that the accumulation of the hyperphosphorylated EF-1\u03b4 is due to phosphorylation by U\n            <jats:sub>L<\/jats:sub>\n            13 protein kinase based on the following observations. (i) The relative amounts of hypo- and hyperphosphorylated EF-1\u03b4 in Vero cells infected with mutant virus lacking the U\n            <jats:sub>L<\/jats:sub>\n            13 gene could not be differentiated from those of mock-infected cells. In contrast, the hyperphosphorylated EF-1\u03b4 was the predominant form in Vero cells infected with wild-type viruses, a recombinant virus in which the deleted U\n            <jats:sub>L<\/jats:sub>\n            13 sequences were restored, or with a virus lacking the U\n            <jats:sub>S<\/jats:sub>\n            3 gene, which also encodes a protein kinase. (ii) The absence of the hyperphosphorylated EF-1\u03b4 in cells infected with the U\n            <jats:sub>L<\/jats:sub>\n            13 deletion mutant was not due to failure of posttranslational modification of infected-cell protein 22 (ICP22)\/U\n            <jats:sub>S<\/jats:sub>\n            1.5 or of interaction with ICP0, inasmuch as preferential accumulation of hyperphosphorylated EF-1\u03b4 was observed in cells infected with viruses from which the genes encoding ICP22\/U\n            <jats:sub>S<\/jats:sub>\n            1.5 or ICP0 had been deleted. (iii) Both forms of EF-1\u03b4 were labeled by\n            <jats:sup>32<\/jats:sup>\n            P\n            <jats:sub>i<\/jats:sub>\n            in vivo, but the prevalence of the hyperphosphorylated EF-1\u03b4 was dependent on the presence of the U\n            <jats:sub>L<\/jats:sub>\n            13 protein. (iv) EF-1\u03b4 immunoprecipitated from uninfected Vero cells was phosphorylated by U\n            <jats:sub>L<\/jats:sub>\n            13 precipitated by the anti-U\n            <jats:sub>L<\/jats:sub>\n            13 antibody from lysates of wild-type virus-infected cells, but not by complexes formed by the interaction of the U\n            <jats:sub>L<\/jats:sub>\n            13 antibody with lysates of cells infected with a mutant lacking the U\n            <jats:sub>L<\/jats:sub>\n            13 gene. This is the first evidence that a viral protein kinase targets a cellular protein. Together with evidence that ICP0 also interacts with EF-1\u03b4 reported in the paper cited above, these data indicate that herpes simplex virus 1 has evolved a complex strategy for optimization of infected-cell protein synthesis.\n          <\/jats:p>","DOI":"10.1128\/jvi.72.3.1731-1736.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T18:08:15Z","timestamp":1577815695000},"page":"1731-1736","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["Eukaryotic Elongation Factor 1\u03b4 Is Hyperphosphorylated by the Protein Kinase Encoded by the U\n            <sub>L<\/sub>\n            13 Gene of Herpes Simplex Virus 1"],"prefix":"10.1128","volume":"72","author":[{"given":"Yasushi","family":"Kawaguchi","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois 60637"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Van Sant","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois 60637"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Roizman","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois 60637"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.1.172-178.1996"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-70-5-1151"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8653"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.1.51"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.56.070187.003031"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-2-3-357"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.3.566"},{"key":"e_1_3_2_9_2","unstructured":"Everett\nR. D.\nPreston\nC. M.\nStow\nN. D.\nFunctional and genetic analysis of the role of Vmw 110 in herpes simplex virus replication\nHerpesvirus transcription and its regulation.\nWagner\nE. K.\n1991\n50\n76\nCRC Press\nBoston Mass"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1073\/pnas.94.3.843","article-title":"The \u03b3134.5 protein of herpes simplex virus 1 complexes with protein phosphatase 1\u03b1 to dephosphorylate the \u03b1 subunit of the eukaryotic translation initiation factor 2 and preclude the shutoff of protein synthesis by double-stranded RNA-activated protein kinase","volume":"94","author":"He B.","year":"1997","unstructured":"He B. Gross M. Roizman B. The \u03b3134.5 protein of herpes simplex virus 1 complexes with protein phosphatase 1\u03b1 to dephosphorylate the \u03b1 subunit of the eukaryotic translation initiation factor 2 and preclude the shutoff of protein synthesis by double-stranded RNA-activated protein kinase.Proc. Natl. Acad. Sci. USA 94 1997 843 848","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.14.1.8-19.1974"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.4.1276"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)88880-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.2.1019-1024.1997"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.10.7328-7336.1997"},{"key":"e_1_3_2_16_2","unstructured":"Knipe\nD. K.\nVirus-host cell interactions\nVirology\n3rd ed.\nFields\nB. N.\nKnipe\nD. M.\nHowley\nP.\nChanock\nR. M.\nHirsch\nM. S.\nMelnick\nJ. L.\nMonath\nT. P.\nRoizman\nB.\n1996\n273\n299\nRaven Press\nNew York N.Y"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.15.7891"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.4.1765"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.56.2.291-315.1992"},{"key":"e_1_3_2_20_2","first-page":"521","article-title":"cdc2 kinase sets a memory phosphorylation signal on elongation factor EF-1\u03b4 during meiotic cell division, which perdures in early development","volume":"40","author":"Minella O.","year":"1994","unstructured":"Minella O. Cormier P. Morales J. Poulhe R. Belle R. Mulner-Lorillon O. cdc2 kinase sets a memory phosphorylation signal on elongation factor EF-1\u03b4 during meiotic cell division, which perdures in early development.Cell. Mol. Biol. 40 1994 521 525","journal-title":"Cell. Mol. Biol."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.15.4091"},{"key":"e_1_3_2_22_2","unstructured":"Moss\nB. Poxviridae : the viruses and their replication\nVirology\n3rd ed.\nFields\nB. N.\nKnipe\nD. M.\nHowley\nP.\nChanock\nR. M.\nHirsch\nM. S.\nMelnick\nJ. L.\nMonath\nT. P.\nRoizman\nB.\n1996\n2637\n2671\nRaven Press\nNew York N.Y"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32146-4"},{"key":"e_1_3_2_24_2","unstructured":"Nevins\nJ. R.\nVogt\nP. K.\nCell transformation by viruses\nVirology\n3rd ed.\nFields\nB. N.\nKnipe\nD. M.\nHowley\nP.\nChanock\nR. M.\nHirsch\nM. S.\nMelnick\nJ. L.\nMonath\nT. P.\nRoizman\nB.\n1996\n301\n343\nRaven Press\nNew York N.Y"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8963"},{"key":"e_1_3_2_26_2","unstructured":"Ogle W. O. T. I. Ng K. L. Carter and B. Roizman. The U L 13 protein kinase and the infected cell type are determinants of posttranslational modification of ICP0. Virology in press."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00194a016"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90247-6"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.14.6701"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.16.7310"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.11.5757-5764.1991"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90304-9"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(90)90279-K"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.11307"},{"key":"e_1_3_2_35_2","unstructured":"Roizman\nB.\nSears\nA. E.\nThe replication of herpes simplex viruses\nVirology\n3rd ed.\nFields\nB. N.\nKnipe\nD. M.\nHowley\nP.\nChanock\nR. M.\nHirsch\nM. S.\nMelnick\nJ. L.\nMonath\nT. P.\nRoizman\nB.\n1996\n2231\n2295\nRaven Press\nNew York N.Y"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.63.1.450-455.1989"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4781(90)90174-Z"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99055-1"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)98937-4"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90303-7"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.72.3.1731-1736.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.72.3.1731-1736.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T02:45:50Z","timestamp":1646448350000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.72.3.1731-1736.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1998,3]]}},"alternative-id":["10.1128\/JVI.72.3.1731-1736.1998"],"URL":"https:\/\/doi.org\/10.1128\/jvi.72.3.1731-1736.1998","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3]]},"assertion":[{"value":"1997-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-11-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}