{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T16:30:29Z","timestamp":1773246629872,"version":"3.50.1"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1998,7,1]],"date-time":"1998-07-01T00:00:00Z","timestamp":899251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[1998,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A 100-fold increase in luciferase activity was observed in 293 cells, stably expressing Epstein-Barr nuclear antigen 1 (EBNA1; 293-EBNA1 cells), that had been transiently transfected with plasmids carrying Epstein-Barr virus (EBV)\n            <jats:italic>oriP<\/jats:italic>\n            sequences. This increase was observed in comparison to reporter gene activity obtained after transfection with a plasmid carrying no\n            <jats:italic>oriP<\/jats:italic>\n            sequences. The luciferase gene on these plasmids was under the control of either the cytomegalovirus immediate-early 1 gene enhancer-promoter (CMV IE1) or the Rous sarcoma virus promoter. The increase of reporter gene activity was not due to plasmid replication, since a similar enhancement was observed in the presence of aphidicolin, an inhibitor of replicative DNA synthesis, or after deletion of the dyad symmetry (\n            <jats:italic>DS<\/jats:italic>\n            ) element within\n            <jats:italic>oriP<\/jats:italic>\n            . Luciferase production was not increased in the presence of only the\n            <jats:italic>DS<\/jats:italic>\n            element. Microinjection of plasmids carrying the CMV IE1 promoter-driven luciferase gene with or without\n            <jats:italic>oriP<\/jats:italic>\n            sequences into the nuclei of 293-EBNA1 cells resulted in a 17-fold increase in luciferase activity. Cytoplasmic injection of these plasmids led to an enhancement of luciferase activity of up to 100-fold. This difference in the factor of activation after nuclear or cytoplasmic injection could be ascribed to increased transport of plasmids carrying\n            <jats:italic>oriP<\/jats:italic>\n            from the cytoplasm to the nucleus in the presence of EBNA1. These data suggest the possibility of substantially increasing the apparent expression of a gene under the control of a strong constitutive promoter in the presence of\n            <jats:italic>oriP<\/jats:italic>\n            sequences and EBNA1. This improvement in expression is due to intranuclear enhancement of gene expression.\n            <jats:italic>oriP<\/jats:italic>\n            -specific transport of plasmid DNA from the cytoplasm of 293-EBNA1 cells to the nucleus seems to contribute to the observed effect.\n          <\/jats:p>","DOI":"10.1128\/jvi.72.7.6181-6185.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T18:15:49Z","timestamp":1577816149000},"page":"6181-6185","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":109,"title":["Up to 100-Fold Increase of Apparent Gene Expression in the Presence of Epstein-Barr Virus\n            <i>oriP<\/i>\n            Sequences and EBNA1: Implications of the Nuclear Import of Plasmids"],"prefix":"10.1128","volume":"72","author":[{"given":"Franc\u0327oise","family":"La\u0308ngle-Rouault","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Transge\u0300ne S.A., 67000 Strasbourg, France,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volker","family":"Patzel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Deutsches Krebsforschungszentrum, Forschungsschwerpunkt Angewandte Tumorvirologie, 69120 Heidelberg, Germany2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annie","family":"Benavente","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Transge\u0300ne S.A., 67000 Strasbourg, France,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Taillez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Transge\u0300ne S.A., 67000 Strasbourg, France,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Silvestre","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Transge\u0300ne S.A., 67000 Strasbourg, France,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albine","family":"Bompard","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Transge\u0300ne S.A., 67000 Strasbourg, France,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georg","family":"Sczakiel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Deutsches Krebsforschungszentrum, Forschungsschwerpunkt Angewandte Tumorvirologie, 69120 Heidelberg, Germany2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Jacobs","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Transge\u0300ne S.A., 67000 Strasbourg, France,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karola","family":"Rittner","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Transge\u0300ne S.A., 67000 Strasbourg, France,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.5.2369-2379.1990"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.3.1466-1478.1991"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/310207a0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(85)80025-8"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1996.3427"},{"key":"e_1_3_2_7_2","first-page":"725","article-title":"Firefly luciferase gene: structure and expression in mammalian cells","volume":"7","author":"DeWet J. 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