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Forty-five patients in a Spanish tertiary care hospital were studied (1996 to 2003). The diversity of In60 and association of In60 with Tn\n            <jats:italic>402<\/jats:italic>\n            or mercury resistance transposons were investigated by overlapping PCR assays and\/or hybridization. Plasmid characterization included comparison of restriction fragment length polymorphism patterns and determination of incompatibility group by PCR-based replicon typing, sequencing, and hybridization. CTX-M-9 plasmids belonged to IncHI2 (\n            <jats:italic>n<\/jats:italic>\n            = 26), IncP-1\u03b1 (\n            <jats:italic>n<\/jats:italic>\n            = 10), IncFI (\n            <jats:italic>n<\/jats:italic>\n            = 4), and IncI (\n            <jats:italic>n<\/jats:italic>\n            = 1) groups. Genetic platforms containing\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-9<\/jats:sub>\n            were classified in six types in relation to the In60 backbone and in eight subtypes in relation to Tn\n            <jats:italic>402<\/jats:italic>\n            derivatives. They were associated with Tn\n            <jats:italic>21<\/jats:italic>\n            sequences when located in IncP-1\u03b1 or IncHI2 plasmids. Our study identified\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-9<\/jats:sub>\n            in a high diversity of CR1-bearing class 1 integrons linked to different Tn\n            <jats:italic>402<\/jats:italic>\n            derivatives, often to Tn\n            <jats:italic>21<\/jats:italic>\n            , highlighting the role of recombination events in the evolution of antibiotic resistance plasmids. The presence of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-9<\/jats:sub>\n            on broad-host-range IncP-1\u03b1 plasmids might contribute to its dissemination to hosts that were not members of the family\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.00274-06","type":"journal-article","created":{"date-parts":[[2006,7,26]],"date-time":"2006-07-26T18:17:58Z","timestamp":1153937878000},"page":"2741-2750","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":96,"title":["Dissemination and Persistence of\n            <i>bla<\/i>\n            <sub>CTX-M-9<\/sub>\n            Are Linked to Class 1 Integrons Containing CR1 Associated with Defective Transposon Derivatives from Tn\n            <i>402<\/i>\n            Located in Early Antibiotic Resistance Plasmids of IncHI2, IncP1-\u03b1, and IncFI Groups"],"prefix":"10.1128","volume":"50","author":[{"given":"A\u0302ngela","family":"Novais","sequence":"first","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"}]},{"given":"Rafael","family":"Canto\u0301n","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"},{"name":"Unidad de Resistencia a Antibio\u0301ticos y Virulencia Bacteriana, Consejo Superior de Investigaciones Cienti\u0301ficas (CSIC), Madrid, Spain"}]},{"given":"Ara\u0301nzazu","family":"Valverde","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"}]},{"given":"Elisabete","family":"Machado","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"},{"name":"Faculdade de Farma\u0301cia, Universidade do Porto, Porto, Portugal;"}]},{"given":"Juan-Carlos","family":"Gala\u0301n","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"},{"name":"Unidad de Resistencia a Antibio\u0301ticos y Virulencia Bacteriana, Consejo Superior de Investigaciones Cienti\u0301ficas (CSIC), Madrid, Spain"}]},{"given":"Lui\u0301sa","family":"Peixe","sequence":"additional","affiliation":[{"name":"Faculdade de Farma\u0301cia, Universidade do Porto, Porto, Portugal;"}]},{"given":"Alessandra","family":"Carattoli","sequence":"additional","affiliation":[{"name":"Istituto Superiore di Sanita\u0300, Roma, Italy"}]},{"given":"Fernando","family":"Baquero","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"},{"name":"Unidad de Resistencia a Antibio\u0301ticos y Virulencia Bacteriana, Consejo Superior de Investigaciones Cienti\u0301ficas (CSIC), Madrid, Spain"}]},{"given":"Teresa M.","family":"Coque","sequence":"additional","affiliation":[{"name":"Hospital Universitario Ramo\u0301n y Cajal, IMSALUD, Madrid, Spain"},{"name":"Unidad de Resistencia a Antibio\u0301ticos y Virulencia Bacteriana, Consejo Superior de Investigaciones Cienti\u0301ficas (CSIC), Madrid, Spain"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro909"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1220"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1-14.2004"},{"key":"e_1_3_2_5_2","first-page":"17314","volume":"46","year":"2002","unstructured":"Boyd, D., A. 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