{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T18:36:11Z","timestamp":1774982171805,"version":"3.50.1"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2007,9]]},"abstract":"ABSTRACT<\/jats:title>\n \n Staphylococcal cassette chromosome\n mec<\/jats:italic>\n (SCC\n mec<\/jats:italic>\n ) typing is important for the identification and definition of methicillin-resistant\n Staphylococcus aureus<\/jats:italic>\n clones, and for routine purposes, multiplex PCR assays are the most adequate for SCC\n mec<\/jats:italic>\n typing. Here, we describe an update to the multiplex PCR strategy for SCC\n mec<\/jats:italic>\n typing that we described in 2002 so that SCC\n mec<\/jats:italic>\n types IV and V may be properly identified.\n <\/jats:p>","DOI":"10.1128\/aac.00275-07","type":"journal-article","created":{"date-parts":[[2007,6,19]],"date-time":"2007-06-19T00:59:44Z","timestamp":1182214784000},"page":"3374-3377","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":410,"title":["Update to the Multiplex PCR Strategy for Assignment of\n mec<\/i>\n Element Types in\n Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"51","author":[{"given":"Catarina","family":"Milheiric\u0327o","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Genetics, Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Oeiras, Portugal"}]},{"given":"Duarte C.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Oeiras, Portugal"}]},{"given":"Hermi\u0301nia","family":"de Lencastre","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Oeiras, Portugal"},{"name":"Laboratory of Microbiology, The Rockefeller University, New York, New York 10021"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)08713-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.122108599"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.5.1323-1336.2001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.6.1449"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.7.2637-2651.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-7646(03)00003-7"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.6.1549-1555.2000"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki306"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.4.1147-1152.2002"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.5113-5120.2003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm112"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4289-4294.2002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.7.2155-2161.2002"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00629-06"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl208"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/10766290152773365"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.5.2070-2083.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1304.061316"}],"updated-by":[{"DOI":"10.1128\/aac.01362-07","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000}}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00275-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00275-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T19:25:59Z","timestamp":1645471559000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00275-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":18,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1128\/AAC.00275-07"],"URL":"https:\/\/doi.org\/10.1128\/aac.00275-07","relation":{"correction":[{"id-type":"doi","id":"10.1128\/AAC.01362-07","asserted-by":"object"}]},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9]]},"assertion":[{"value":"2007-02-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-06-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}