{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:09:28Z","timestamp":1771632568144,"version":"3.50.1"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            PAO1 gene\n            <jats:italic>pvdQ<\/jats:italic>\n            encodes an acyl-homoserine lactone (AHL) acylase capable of degrading\n            <jats:italic>N<\/jats:italic>\n            -(3-oxododecanoyl)-\n            <jats:sc>l<\/jats:sc>\n            -homoserine lactone by cleaving the AHL amide. PvdQ has been proven to function as a quorum quencher in vitro in a number of phenotypic assays. To address the question of whether PvdQ also shows quorum-quenching properties in vivo, an infection model based on the nematode\n            <jats:italic>Caenorhabditis elegans<\/jats:italic>\n            was explored. In a fast-acting paralysis assay, strain PAO1(pME\n            <jats:italic>pvdQ<\/jats:italic>\n            ), which overproduces PvdQ, was shown to be less virulent than the wild-type strain. More than 75% of the nematodes exposed to PAO1(pME\n            <jats:italic>pvdQ<\/jats:italic>\n            ) survived and continued to grow when using this strain as a food source. Interestingly, in a slow-killing assay monitoring the survival of the nematodes throughout a 4-day course, strain PAO1-\u0394\n            <jats:italic>pvdQ<\/jats:italic>\n            was shown to be more virulent than the wild-type strain, confirming the role of PvdQ as a virulence-reducing agent. It was observed that larval stage 1 (L1) to L3-stage larvae benefit much more from protection by PvdQ than L4 worms. Finally, purified PvdQ protein was added to\n            <jats:italic>C. elegans<\/jats:italic>\n            worms infected with wild-type PAO1, and this resulted in reduced pathogenicity and increased the life span of the nematodes. From our observations we can conclude that PvdQ might be a strong candidate for antibacterial therapy against\n            <jats:italic>Pseudomonas<\/jats:italic>\n            infections.\n          <\/jats:p>","DOI":"10.1128\/aac.00380-09","type":"journal-article","created":{"date-parts":[[2009,9,1]],"date-time":"2009-09-01T01:36:01Z","timestamp":1251768961000},"page":"4891-4897","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":96,"title":["Quorum-Quenching Acylase Reduces the Virulence of\n            <i>Pseudomonas aeruginosa<\/i>\n            in a\n            <i>Caenorhabditis elegans<\/i>\n            Infection Model"],"prefix":"10.1128","volume":"53","author":[{"given":"Evelina","family":"Papaioannou","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands"}]},{"given":"Mariana","family":"Wahjudi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands"},{"name":"Faculty of Pharmacy and Faculty of Technobiology, University of Surabaya, Surabaya, Indonesia"}]},{"given":"Pol","family":"Nadal-Jimenez","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands"}]},{"given":"Gudrun","family":"Koch","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands"}]},{"given":"Rita","family":"Setroikromo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands"}]},{"given":"Wim J.","family":"Quax","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, University 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