{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:17:11Z","timestamp":1773659831856,"version":"3.50.1"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The clinical\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            INSRA6884 strain exhibited nonsusceptibility to all penicillins tested (MICs of 64 to &gt;2,048 \u03bcg\/ml). The MICs of penicillins were weakly reduced by clavulanate (from 2,048 to 512 \u03bcg\/ml), and tazobactam restored piperacillin susceptibility. Molecular characterization identified the genes\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-7<\/jats:sub>\n            and a new \u03b2-lactamase gene,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-107<\/jats:sub>\n            , which encoded an enzyme that differed from SHV-1 by the amino acid substitutions Leu35Gln and Thr235Ala. The SHV-107-producing\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            strain exhibited only a \u03b2-lactam resistance phenotype with respect to amoxicillin, ticarcillin, and amoxicillin-clavulanate combination. The kinetic parameters of the purified SHV-107 enzyme revealed a high affinity for penicillins. However, catalytic efficiency for these antibiotics was lower for SHV-107 than for SHV-1. No hydrolysis was detected against oxyimino-\u03b2-lactams. The 50% inhibitory concentration (IC\n            <jats:sub>50<\/jats:sub>\n            ) for clavulanic acid was 9-fold higher for SHV-107 than for SHV-1, but the inhibitory effects of tazobactam were unchanged. Molecular dynamics simulation suggested that the Thr235Ala substitution affects the accommodation of clavulanate in the binding site and therefore its inhibitory activity.\n          <\/jats:p>","DOI":"10.1128\/aac.01444-10","type":"journal-article","created":{"date-parts":[[2011,11,15]],"date-time":"2011-11-15T19:06:06Z","timestamp":1321383966000},"page":"1042-1046","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Characterization of the Inhibitor-Resistant SHV \u03b2-Lactamase SHV-107 in a Clinical Klebsiella pneumoniae Strain Coproducing GES-7 Enzyme"],"prefix":"10.1128","volume":"56","author":[{"given":"Vera","family":"Manageiro","sequence":"first","affiliation":[{"name":"National Reference Laboratory of Antimicrobial Resistances, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal"}]},{"given":"Eug\u00e9nia","family":"Ferreira","sequence":"additional","affiliation":[{"name":"National Reference Laboratory of Antimicrobial Resistances, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal"}]},{"given":"Antony","family":"Cougnoux","sequence":"additional","affiliation":[{"name":"Clermont Universit\u00e9, Universit\u00e9 d'Auvergne, Evolution des Bact\u00e9ries Pathog\u00e8nes et Susceptibilit\u00e9 de l'H\u00f4te, Clermont-Ferrand, France"}]},{"given":"Lu\u00eds","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Centro Hospitalar de Coimbra, EPE, Coimbra, Portugal"}]},{"given":"Manuela","family":"Cani\u00e7a","sequence":"additional","affiliation":[{"name":"National Reference Laboratory of Antimicrobial Resistances, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal"}]},{"given":"Richard","family":"Bonnet","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Laboratoire de Bact\u00e9riologie, Clermont-Ferrand, France"},{"name":"Clermont Universit\u00e9, Universit\u00e9 d'Auvergne, Evolution des Bact\u00e9ries Pathog\u00e8nes et Susceptibilit\u00e9 de l'H\u00f4te, Clermont-Ferrand, France"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1-14.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.8.2269-2275.2001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"e34","DOI":"10.2741\/A477","article-title":"Inhibitor resistant class A \u03b2-lactamases","volume":"4","author":"Bonomo RA","year":"1999","unstructured":"BonomoRA RiceLB. 1999. 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