{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T06:51:56Z","timestamp":1777531916958,"version":"3.51.4"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Since its first description in 2000, CTX-M-14 has become one of the most widespread extended-spectrum \u03b2-lactamases in Spain. In the present\n            <jats:italic>Escherichia coli<\/jats:italic>\n            multilevel population genetic study involving the characterization of phylogroups, clones, plasmids, and genetic platforms, 61 isolates from 16 hospitalized patients and 40 outpatients and healthy volunteers recovered from 2000 to 2005 were analyzed. Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] type, phylogenetic group, multilocus sequence type [MLST]) was established by standard methods. Analysis of transferred plasmids (I-\n            <jats:italic>Ceu<\/jats:italic>\n            I; S1 nuclease; restriction fragment length polymorphism analysis; and analysis of RNA interference, replicase, and relaxase) was performed by PCR, sequencing, and hybridization. The genetic environment of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14<\/jats:sub>\n            was characterized by PCR on the basis of known associated structures (IS\n            <jats:italic>Ecp1<\/jats:italic>\n            , IS\n            <jats:italic>903<\/jats:italic>\n            , IS\n            <jats:italic>CR1<\/jats:italic>\n            ). The isolates were mainly recovered from patients in the community (73.8%; 45\/61) with urinary tract infections (62.2%; 28\/45). They were clonally unrelated by PFGE and corresponded to phylogenetic groups A (36.1%), D (34.4%), and B1 (29.5%). MLST revealed a high degree of sequence type (ST) diversity among phylogroup D isolates and the overrepresentation of the ST10 complex among phylogroup A isolates and ST359\/ST155 among phylogroup B1 isolates. Two variants of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            <jats:sub>-14<\/jats:sub>\n            previously designated\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14a<\/jats:sub>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 59\/61) and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14b<\/jats:sub>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 2\/61) were detected.\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14a<\/jats:sub>\n            was associated with either IS\n            <jats:italic>Ecp1<\/jats:italic>\n            within IncK plasmids (\n            <jats:italic>n<\/jats:italic>\n            = 27), IS\n            <jats:italic>CR1<\/jats:italic>\n            linked to an IncHI2 plasmid (\n            <jats:italic>n<\/jats:italic>\n            = 1), or IS\n            <jats:italic>CR1<\/jats:italic>\n            linked to IncI-like plasmids (\n            <jats:italic>n<\/jats:italic>\n            = 3). The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14b<\/jats:sub>\n            identified was associated with an IS\n            <jats:italic>CR1<\/jats:italic>\n            element located in an IncHI2 plasmid (\n            <jats:italic>n<\/jats:italic>\n            = 1) or with IS\n            <jats:italic>Ecp1<\/jats:italic>\n            located in IncK (\n            <jats:italic>n<\/jats:italic>\n            = 1). The CTX-M-14-producing\n            <jats:italic>E. coli<\/jats:italic>\n            isolates in our geographic area are frequent causes of community-acquired urinary tract infections. The increase in the incidence of such isolates is mostly due to the dissemination of IncK plasmids among\n            <jats:italic>E. coli<\/jats:italic>\n            isolates of phylogroups A, B1, and D.\n          <\/jats:p>","DOI":"10.1128\/aac.01706-08","type":"journal-article","created":{"date-parts":[[2009,9,29]],"date-time":"2009-09-29T01:16:33Z","timestamp":1254186993000},"page":"5204-5212","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":111,"title":["Spread of\n            <i>bla<\/i>\n            <sub>CTX-M-14<\/sub>\n            Is Driven Mainly by IncK Plasmids Disseminated among\n            <i>Escherichia coli<\/i>\n            Phylogroups A, B1, and D in Spain"],"prefix":"10.1128","volume":"53","author":[{"given":"Ara\u0301nzazu","family":"Valverde","sequence":"first","affiliation":[{"name":"Servicio de Microbiologi\u0301a del Hospital Universitario Ramo\u0301n y Cajal, Madrid, Spain"},{"name":"CIBER Epidemiologi\u0301a y Salud Pu\u0301blica, Madrid, Spain"}]},{"given":"Rafael","family":"Canto\u0301n","sequence":"additional","affiliation":[{"name":"Servicio de Microbiologi\u0301a del Hospital Universitario Ramo\u0301n y Cajal, Madrid, Spain"},{"name":"CIBER Epidemiologi\u0301a y Salud Pu\u0301blica, Madrid, Spain"},{"name":"Unidad de Resistencia a Antibio\u0301ticos y Virulencia Bacteriana Asociada al Consejo Superior de Investigaciones Cienti\u0301ficas, Madrid, Spain"}]},{"given":"M. Pilar","family":"Garcilla\u0301n-Barcia","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a Molecular e Instituto de Biomedicina y Biotecnologi\u0301a de Cantabria, Universidad de Cantabria-Consejo Superior de Investigaciones Cienti\u0301ficas-IDICAN, C. Herrera Oria s\/n, Santander 39011, Spain"}]},{"given":"A\u0302ngela","family":"Novais","sequence":"additional","affiliation":[{"name":"Servicio de Microbiologi\u0301a del Hospital Universitario Ramo\u0301n y Cajal, Madrid, Spain"},{"name":"CIBER Epidemiologi\u0301a y Salud Pu\u0301blica, Madrid, Spain"}]},{"given":"Juan Carlos","family":"Gala\u0301n","sequence":"additional","affiliation":[{"name":"Servicio de Microbiologi\u0301a del Hospital Universitario Ramo\u0301n y Cajal, Madrid, Spain"},{"name":"CIBER Epidemiologi\u0301a y Salud Pu\u0301blica, Madrid, Spain"},{"name":"Unidad de Resistencia a Antibio\u0301ticos y Virulencia Bacteriana Asociada al Consejo Superior de Investigaciones Cienti\u0301ficas, Madrid, Spain"}]},{"given":"Andre\u0301s","family":"Alvarado","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a Molecular e Instituto de Biomedicina y Biotecnologi\u0301a de Cantabria, Universidad de Cantabria-Consejo Superior de Investigaciones Cienti\u0301ficas-IDICAN, C. Herrera Oria s\/n, Santander 39011, Spain"}]},{"given":"Fernando","family":"de la Cruz","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a Molecular e Instituto de Biomedicina y Biotecnologi\u0301a de Cantabria, Universidad de Cantabria-Consejo Superior de Investigaciones Cienti\u0301ficas-IDICAN, C. Herrera Oria s\/n, Santander 39011, Spain"}]},{"given":"Fernando","family":"Baquero","sequence":"additional","affiliation":[{"name":"Servicio de Microbiologi\u0301a del Hospital Universitario Ramo\u0301n y Cajal, Madrid, Spain"},{"name":"CIBER Epidemiologi\u0301a y Salud Pu\u0301blica, Madrid, Spain"},{"name":"Unidad de Resistencia a Antibio\u0301ticos y Virulencia Bacteriana Asociada al Consejo Superior de Investigaciones Cienti\u0301ficas, Madrid, Spain"},{"name":"Fundacio\u0301n para la Investigacio\u0301n en Biomedicina del Hospital Universitario Ramo\u0301n y Cajal (FiBIO-HRYC), Madrid, Spain"}]},{"given":"Teresa M.","family":"Coque","sequence":"additional","affiliation":[{"name":"Servicio de Microbiologi\u0301a del Hospital Universitario Ramo\u0301n y Cajal, Madrid, Spain"},{"name":"CIBER Epidemiologi\u0301a y Salud Pu\u0301blica, Madrid, Spain"},{"name":"Unidad de Resistencia a Antibio\u0301ticos y Virulencia Bacteriana Asociada al Consejo Superior de Investigaciones Cienti\u0301ficas, Madrid, Spain"},{"name":"Fundacio\u0301n para la Investigacio\u0301n en Biomedicina del Hospital Universitario Ramo\u0301n y Cajal (FiBIO-HRYC), Madrid, Spain"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"134","year":"2008","unstructured":"Alvarado, A., M. P. Garcill\u00e1n-Barcia, and F. de la Cruz. 2008. A PCR-based method for classification of conjugative plasmids based on relaxase sequences, p. 134. InProceedings of the International Plasmid Biology Conference. International Plasmid Biology Society, Gdansk, Poland.","journal-title":"Proceedings of the International Plasmid Biology Conference."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1983.tb03560.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00279-07"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro909"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1403.070405"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4030-4036.2002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00640-08"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.08.011"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2008.0827"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"e_1_3_2_13_2","unstructured":"Performance standards for antimicrobial susceptibility testing. 2006"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.13.47.19044-en"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.52.3.375-395.1988"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.2.434-464.1998"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn453"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2102-2114.2006"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki398"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msh118"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2009.00168.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2008.01669.x"},{"key":"e_1_3_2_23_2","first-page":"220","volume":"234","year":"1971","unstructured":"Hedges, R. W., and N. Datta. 1971. fi\u2212 R factors giving chloramphenicol resistance. Nature234:220-221.","journal-title":"Nature"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.5.2122-2125.2005"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm144"},{"key":"e_1_3_2_26_2","first-page":"560","volume":"9","year":"2009","unstructured":"Jaureguy, F., L. Landraud, V. Passet, L. Diancourt, E. Frapy, G. Guigon, E. Carbonnelle, O. Lortholary, O. Clermont, E. Denamur, B. Picard, X. Nassif, and S. Brisse. 2009. Phylogenetic and genomic diversity of human bacteremic Escherichia coli strains. BMC Genomics9:560.","journal-title":"BMC Genomics"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00171-08"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2008.03.005"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.2.150"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02065-07"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00734-08"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00123-09"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.5.1630-1634.2006"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp068"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00852-08"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn428"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.4.647-679.2002"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01412-06"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-007-9026-3"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.4.1204-1214.2004"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00791.x"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01070-06"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00274-06"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-9-2267"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dsn026"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.03.006"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.10.3747-3749.2001"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2844-2849.2005"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4667-4670.2005"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.1089-1094.2004"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn463"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.12.5860-5864.2005"},{"key":"e_1_3_2_53_2","first-page":"515","volume":"118","year":"1980","unstructured":"Tsch\u00e4pe, H., and E. Tietze. 1980. Genetic and molecular characterization of R plasmids incompatible with R387 (IncK). J. Gen. Microbiol.118:515-521.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01474-06"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4769-4775.2004"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01008-08"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02112.x"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05172.x"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh424"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01706-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01706-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T19:40:24Z","timestamp":1645472424000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01706-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12]]},"references-count":58,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2009,12]]}},"alternative-id":["10.1128\/AAC.01706-08"],"URL":"https:\/\/doi.org\/10.1128\/aac.01706-08","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12]]},"assertion":[{"value":"2008-12-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-09-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}