{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T16:16:49Z","timestamp":1776010609299,"version":"3.50.1"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T00:00:00Z","timestamp":1306886400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            During blood infection, malarial parasites use\n            <jats:sc>d<\/jats:sc>\n            -glucose as their main energy source. The\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Plasmodium falciparum<\/jats:named-content>\n            hexose transporter (PfHT), which mediates the uptake of\n            <jats:sc>d<\/jats:sc>\n            -glucose into parasites, is essential for survival of asexual blood-stage parasites. Recently, genetic studies in the rodent malaria model,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Plasmodium berghei<\/jats:named-content>\n            , found that the orthologous hexose transporter (PbHT) is expressed throughout the parasite's development within the mosquito vector, in addition to being essential during intraerythrocytic development. Here, using a\n            <jats:sc>d<\/jats:sc>\n            -glucose-derived specific inhibitor of plasmodial hexose transporters, compound 3361, we have investigated the importance of\n            <jats:sc>d<\/jats:sc>\n            -glucose uptake during liver and transmission stages of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. berghei<\/jats:named-content>\n            . Initially, we confirmed the expression of PbHT during liver stage development, using a green fluorescent protein (GFP) tagging strategy. Compound 3361 inhibited liver-stage parasite development, with a 50% inhibitory concentration (IC\n            <jats:sub>50<\/jats:sub>\n            ) of 11 \u03bcM. This process was insensitive to the external\n            <jats:sc>d<\/jats:sc>\n            -glucose concentration. In addition, compound 3361 inhibited ookinete development and microgametogenesis, with IC\n            <jats:sub>50<\/jats:sub>\n            s in the region of 250 \u03bcM (the latter in a\n            <jats:sc>d<\/jats:sc>\n            -glucose-sensitive manner). Consistent with our findings for the effect of compound 3361 on vector parasite stages, 1 mM compound 3361 demonstrated transmission blocking activity. These data indicate that novel chemotherapeutic interventions that target PfHT may be active against liver and, to a lesser extent, transmission stages, in addition to blood stages.\n          <\/jats:p>","DOI":"10.1128\/aac.01739-10","type":"journal-article","created":{"date-parts":[[2011,3,15]],"date-time":"2011-03-15T18:53:46Z","timestamp":1300215226000},"page":"2824-2830","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Use of a Selective Inhibitor To Define the Chemotherapeutic Potential of the Plasmodial Hexose Transporter in Different Stages of the Parasite's Life Cycle"],"prefix":"10.1128","volume":"55","author":[{"given":"Ksenija","family":"Slavic","sequence":"first","affiliation":[{"name":"Centre for Infection and Immunity, Division of Clinical Sciences, St. George's, University of London, London SW17 0RE, United Kingdom"}]},{"given":"Michael J.","family":"Delves","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom"}]},{"given":"Miguel","family":"Prud\u00eancio","sequence":"additional","affiliation":[{"name":"Unidade de Malaria, Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal"}]},{"given":"Arthur M.","family":"Talman","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom"}]},{"given":"Ursula","family":"Straschil","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom"},{"name":"Institute of Genetics, School of Biology, The University of Nottingham, Nottingham, United Kingdom"}]},{"given":"Elvira T.","family":"Derbyshire","sequence":"additional","affiliation":[{"name":"Centre for Infection and Immunity, Division of Clinical Sciences, St. George's, University of London, London SW17 0RE, United Kingdom"}]},{"given":"Zhengyao","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Genetics, School of Biology, The University of Nottingham, Nottingham, United Kingdom"}]},{"given":"Robert E.","family":"Sinden","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom"}]},{"given":"Maria M.","family":"Mota","sequence":"additional","affiliation":[{"name":"Unidade de Malaria, Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal"}]},{"given":"Christophe","family":"Morin","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Chimie Mol\u00e9culaire (UMR 5250, ICMG FR-2607, CNRS), Universit\u00e9 Joseph Fourier, Grenoble Cedex, France"}]},{"given":"Rita","family":"Tewari","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom"},{"name":"Institute of Genetics, School of Biology, The University of Nottingham, Nottingham, United Kingdom"}]},{"given":"Sanjeev","family":"Krishna","sequence":"additional","affiliation":[{"name":"Centre for Infection and Immunity, Division of Clinical Sciences, St. George's, University of London, London SW17 0RE, United Kingdom"}]},{"given":"Henry M.","family":"Staines","sequence":"additional","affiliation":[{"name":"Centre for Infection and Immunity, Division of Clinical Sciences, St. George's, University of London, London SW17 0RE, United Kingdom"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00238.2004"},{"key":"e_1_3_2_3_2","first-page":"183","volume-title":"Facilitative glucose transporters","author":"Brot-Laroche E.","year":"1997","unstructured":"Brot-LarocheE. 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