{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T19:37:30Z","timestamp":1766518650197,"version":"3.40.5"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n This work describes the diversity and evolution of Tn\n 5801<\/jats:italic>\n among enterococci, staphylococci, and streptococci based on analysis of the 5,073 genomes of these bacterial groups available in gene databases. We also examined 610 isolates of\n Enterococcus<\/jats:named-content>\n (from 10 countries, 1987 to 2010) for the presence of this and other known CTn-\n tet<\/jats:italic>\n (M) elements due to the scarcity of data about Tn\n 5801<\/jats:italic>\n among enterococci. Genome location (by I\n Ceu<\/jats:italic>\n -I\u2013pulsed-field gel electrophoresis [PFGE] hybridization\/integration site identification), conjugation and fitness (by standard methods), Tn\n 5801<\/jats:italic>\n characterization (by long-PCR mapping\/sequencing), and clonality (by PFGE\/multilocus sequence typing [MLST]) were studied. Twenty-three Tn\n 5801<\/jats:italic>\n variants (17 unpublished) clustered in two groups, designated \u201cA\u201d (25 kb;\n n<\/jats:italic>\n = 14; predominant in\n Staphylococcus aureus<\/jats:named-content>\n ) and \u201cB\u201d (20 kb;\n n<\/jats:italic>\n = 9; predominant in\n Streptococcus agalactiae<\/jats:named-content>\n ). The percent GC content of the common backbone suggests a streptococcal origin of Tn\n 5801<\/jats:italic>\n group B, with further acquisition of a 5-kb fragment that resulted in group A. Deep sequence analysis allowed identification of variants associated with clonal lineages of\n S. aureus<\/jats:named-content>\n (clonal complex 8 [CC8], sequence type 239 [ST239]),\n S. agalactiae<\/jats:named-content>\n (CC17),\n Enterococcus faecium<\/jats:named-content>\n (ST17\/ST18), or\n Enterococcus faecalis<\/jats:named-content>\n (ST8), local variants, or variants located in different species and geographical areas. All Tn\n 5801<\/jats:italic>\n elements were chromosomally located upstream of the\n guaA<\/jats:italic>\n gene, which serves as an integration hot spot. Transferability was demonstrated only for Tn\n 5801<\/jats:italic>\n type B among\n E. faecalis<\/jats:named-content>\n clonal backgrounds, which eventually harbored another Tn\n 5801<\/jats:italic>\n copy. The study documents early acquisition of Tn\n 5801<\/jats:italic>\n by\n Enterococcus<\/jats:named-content>\n ,\n Staphylococcus<\/jats:named-content>\n , and\n Streptococcus<\/jats:named-content>\n . Clonal waves of these pathogens seem to have contributed to the geographical spread and local evolution of the transposon. Horizontal transfer, also demonstrated, could explain the variability observed, with the isolates often containing sequences of different origins.\n <\/jats:p>","DOI":"10.1128\/aac.01864-15","type":"journal-article","created":{"date-parts":[[2016,1,5]],"date-time":"2016-01-05T01:59:34Z","timestamp":1451959174000},"page":"1736-1746","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Diversity and Evolution of the Tn\n 5801-tet<\/i>\n (M)-Like Integrative and Conjugative Elements among Enterococcus, Streptococcus, and Staphylococcus"],"prefix":"10.1128","volume":"60","author":[{"given":"Ricardo","family":"Le\u00f3n-Sampedro","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Ram\u00f3n y Cajal, Madrid, Spain"},{"name":"CIBER Epidemiolog\u00eda y Salud P\u00fablica, Madrid, Spain"}]},{"given":"Carla","family":"Novais","sequence":"additional","affiliation":[{"name":"UCIBIO\/REQUIMTE, Departamento de Ci\u00eancias Biol\u00f3gicas, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Lu\u00edsa","family":"Peixe","sequence":"additional","affiliation":[{"name":"UCIBIO\/REQUIMTE, Departamento de Ci\u00eancias Biol\u00f3gicas, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Fernando","family":"Baquero","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Ram\u00f3n y Cajal, Madrid, Spain"},{"name":"Unidad de Resistencia a Antibi\u00f3ticos y Virulencia Bacteriana asociada al Consejo Superior de Investigaciones Cient\u00edficas, Madrid, Spain"},{"name":"CIBER Epidemiolog\u00eda y Salud P\u00fablica, Madrid, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8228-3491","authenticated-orcid":false,"given":"Teresa M.","family":"Coque","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Ram\u00f3n y Cajal, Madrid, Spain"},{"name":"Unidad de Resistencia a Antibi\u00f3ticos y Virulencia Bacteriana asociada al Consejo Superior de Investigaciones Cient\u00edficas, Madrid, Spain"},{"name":"CIBER Epidemiolog\u00eda y Salud P\u00fablica, Madrid, Spain"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5544"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)61651-6"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp214"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn255"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00283.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.8.1871"},{"key":"e_1_3_3_8_2","volume-title":"Enterococci: from commensals to leading causes of drug resistant infection","author":"Clewell DB","year":"2014","unstructured":"Clewell DB, Weaver KE, Dunny GM, Coque TM, Francia MV, Hayes F. 2014. 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