{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T16:55:30Z","timestamp":1761929730330},"reference-count":41,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Plasmodium falciparum<\/jats:named-content>\n            has the capacity to escape the actions of essentially all antimalarial drugs. ATP-binding cassette (ABC) transporter proteins are known to cause multidrug resistance in a large range of organisms, including the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Apicomplexa<\/jats:named-content>\n            parasites.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. falciparum<\/jats:named-content>\n            genome analysis has revealed two genes coding for the multidrug resistance protein (MRP) type of ABC transporters:\n            <jats:italic>Pfmrp1<\/jats:italic>\n            , previously associated with decreased parasite drug susceptibility, and the poorly studied\n            <jats:italic>Pfmrp2<\/jats:italic>\n            . The role of\n            <jats:italic>Pfmrp2<\/jats:italic>\n            polymorphisms in modulating sensitivity to antimalarial drugs has not been established. We herein report a comprehensive account of the\n            <jats:italic>Pfmrp2<\/jats:italic>\n            genetic variability in 46 isolates from Thailand. A notably high frequency of 2.8 single nucleotide polymorphisms (SNPs)\/kb was identified for this gene, including some novel SNPs. Additionally, we found that\n            <jats:italic>Pfmrp2<\/jats:italic>\n            harbors a significant number of microindels, some previously not reported. We also investigated the potential association of the identified\n            <jats:italic>Pfmrp2<\/jats:italic>\n            polymorphisms with altered\n            <jats:italic>in vitro<\/jats:italic>\n            susceptibility to several antimalarials used in artemisinin-based combination therapy and with parasite clearance time. Association analysis suggested\n            <jats:italic>Pfmrp2<\/jats:italic>\n            polymorphisms modulate the parasite's\n            <jats:italic>in vitro<\/jats:italic>\n            response to quinoline antimalarials, including chloroquine, piperaquine, and mefloquine, and association with\n            <jats:italic>in vivo<\/jats:italic>\n            parasite clearance. In conclusion, our study reveals that the\n            <jats:italic>Pfmrp2<\/jats:italic>\n            gene is the most diverse ABC transporter known in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. falciparum<\/jats:named-content>\n            with a potential role in antimalarial drug resistance.\n          <\/jats:p>","DOI":"10.1128\/aac.03337-14","type":"journal-article","created":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T02:09:54Z","timestamp":1412042994000},"page":"7390-7397","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Complex Polymorphisms in the Plasmodium falciparum Multidrug Resistance Protein 2 Gene and Its Contribution to Antimalarial Response"],"prefix":"10.1128","volume":"58","author":[{"given":"Maria Isabel","family":"Veiga","sequence":"first","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's, PT Government Associate Laboratory, Guimar\u00e3es, Braga, Portugal"},{"name":"Malaria Research Laboratory, Department of Medicine, Karolinska Institutet, Stockholm, Sweden"}]},{"given":"Nuno S.","family":"Os\u00f3rio","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's, PT Government Associate Laboratory, Guimar\u00e3es, Braga, Portugal"}]},{"given":"Pedro Eduardo","family":"Ferreira","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Nanyang Technological University, Singapore"},{"name":"Drug Resistance and Pharmacogenetics, Center for Biodiversity, Functional and Integrative Genomics, Faculdade de Ci\u00eancias, Universidade de Lisboa, Lisbon, Portugal"}]},{"given":"Oscar","family":"Franz\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden"}]},{"given":"Sabina","family":"Dahlstrom","sequence":"additional","affiliation":[{"name":"Malaria Research Laboratory, Department of Medicine, Karolinska Institutet, Stockholm, Sweden"}]},{"given":"J. Koji","family":"Lum","sequence":"additional","affiliation":[{"name":"Harpur College of Arts and Sciences, Binghamton University, The State University of New York, Binghamton, New York, USA"}]},{"given":"Francois","family":"Nosten","sequence":"additional","affiliation":[{"name":"Shoklo Malaria Research Unit, Mae Sot, Tak, Thailand"},{"name":"Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom"}]},{"given":"Jos\u00e9 Pedro","family":"Gil","sequence":"additional","affiliation":[{"name":"Drug Resistance and Pharmacogenetics, Center for Biodiversity, Functional and Integrative Genomics, Faculdade de Ci\u00eancias, Universidade de Lisboa, Lisbon, Portugal"},{"name":"Harpur College of Arts and Sciences, Binghamton University, The State University of New York, Binghamton, New York, USA"},{"name":"Division of Pharmacogenetics, Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"303","article-title":"Memoir of the memorias. 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