{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:04:54Z","timestamp":1770509094139,"version":"3.49.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T00:00:00Z","timestamp":1210809600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Initial rates of sugar uptake (zero-\n            <jats:italic>trans<\/jats:italic>\n            rates) are often measured by incubating yeast cells with radiolabeled sugars for 5 to 30 s and determining the radioactivity entering the cells. The yeast cells used are usually harvested from growth medium, washed, suspended in nutrient-free buffer, and stored on ice before they are assayed. With this method, the specific rates of zero-\n            <jats:italic>trans<\/jats:italic>\n            lactose uptake by\n            <jats:italic>Kluyveromyces lactis<\/jats:italic>\n            or recombinant\n            <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n            strains harvested from lactose fermentations were three- to eightfold lower than the specific rates of lactose consumption during fermentation. No significant extracellular \u03b2-galactosidase activity was detected. The ATP content and adenylate energy charge (EC) of the yeasts were relatively low before the [\n            <jats:sup>14<\/jats:sup>\n            C]lactose uptake reactions were started. A short (1- to 7-min) preincubation of the yeasts with 10 to 30 mM glucose caused 1.5- to 5-fold increases in the specific rates of lactose uptake. These increases correlated with increases in EC (from 0.6 to 0.9) and ATP (from 4 to 8 \u03bcmol\u00b7g dry yeast\n            <jats:sup>\u22121<\/jats:sup>\n            ). Stimulation by glucose affected the transport\n            <jats:italic>V<\/jats:italic>\n            <jats:sub>max<\/jats:sub>\n            values, with smaller increases in\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            values. Similar observations were made for maltose transport, using a brewer's yeast. These findings suggest that the electrochemical proton potential that drives transport through sugar\/H\n            <jats:sup>+<\/jats:sup>\n            symports is significantly lower in the starved yeast suspensions used for zero-\n            <jats:italic>trans<\/jats:italic>\n            assays than in actively metabolizing cells. Zero-\n            <jats:italic>trans<\/jats:italic>\n            assays with such starved yeast preparations can produce results that seriously underestimate the capacity of sugar\/H\n            <jats:sup>+<\/jats:sup>\n            symports. A short exposure to glucose allows a closer approach to the sugar\/H\n            <jats:sup>+<\/jats:sup>\n            symport capacity of actively metabolizing cells.\n          <\/jats:p>","DOI":"10.1128\/aem.00188-08","type":"journal-article","created":{"date-parts":[[2008,4,1]],"date-time":"2008-04-01T00:45:29Z","timestamp":1207010729000},"page":"3076-3084","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Stimulation of Zero-\n            <i>trans<\/i>\n            Rates of Lactose and Maltose Uptake into Yeasts by Preincubation with Hexose To Increase the Adenylate Energy Charge"],"prefix":"10.1128","volume":"74","author":[{"given":"Pedro M. 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