{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:27:09Z","timestamp":1761895629741},"reference-count":55,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The bacterial tyrosine-kinase (BY-kinase) family comprises the major group of bacterial enzymes endowed with tyrosine kinase activity. We previously showed that the BceF protein from\n            <jats:named-content content-type=\"genus-species\">Burkholderia cepacia<\/jats:named-content>\n            IST408 belongs to this BY-kinase family and is involved in the biosynthesis of the exopolysaccharide cepacian. However, little is known about the extent of regulation of this protein kinase activity. In order to examine this regulation, we performed a comparative transcriptome profile between the\n            <jats:italic>bceF<\/jats:italic>\n            mutant and wild-type\n            <jats:named-content content-type=\"genus-species\">B. cepacia<\/jats:named-content>\n            IST408. The analyses led to identification of 630 genes whose expression was significantly changed. Genes with decreased expression in the\n            <jats:italic>bceF<\/jats:italic>\n            mutant were related to stress response, motility, cell adhesion, and carbon and energy metabolism. Genes with increased expression were related to intracellular signaling and lipid metabolism. Mutation of\n            <jats:italic>bceF<\/jats:italic>\n            led to reduced survival under heat shock and UV light exposure, reduced swimming motility, and alteration in biofilm architecture when grown\n            <jats:italic>in vitro<\/jats:italic>\n            . Consistent with some of these phenotypes, the\n            <jats:italic>bceF<\/jats:italic>\n            mutant demonstrated elevated levels of cyclic-di-GMP. Furthermore, BceF contributed to the virulence of\n            <jats:named-content content-type=\"genus-species\">B. cepacia<\/jats:named-content>\n            for larvae of the Greater wax moth,\n            <jats:named-content content-type=\"genus-species\">Galleria mellonella<\/jats:named-content>\n            . Taken together, BceF appears to play a considerable role in many cellular processes, including biofilm formation and virulence. As homologues of BceF occur in a number of pathogenic and plant-associated\n            <jats:named-content content-type=\"genus-species\">Burkholderia<\/jats:named-content>\n            strains, the modulation of bacterial behavior through tyrosine kinase activity is most likely a widely occurring phenomenon.\n          <\/jats:p>","DOI":"10.1128\/aem.00222-13","type":"journal-article","created":{"date-parts":[[2013,2,23]],"date-time":"2013-02-23T12:18:49Z","timestamp":1361621929000},"page":"3009-3020","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Comparative Transcriptomic Analysis of the Burkholderia cepacia Tyrosine Kinase\n            <i>bceF<\/i>\n            Mutant Reveals a Role in Tolerance to Stress, Biofilm Formation, and Virulence"],"prefix":"10.1128","volume":"79","author":[{"given":"Ana S.","family":"Ferreira","sequence":"first","affiliation":[{"name":"Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Lisbon, Portugal"}]},{"given":"In\u00eas N.","family":"Silva","sequence":"additional","affiliation":[{"name":"Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Lisbon, Portugal"}]},{"given":"V\u00edtor H.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Lisbon, Portugal"}]},{"given":"J\u00f6rg D.","family":"Becker","sequence":"additional","affiliation":[{"name":"Instituto Gulbenkian de Ci\u00eancia, Oeiras, Portugal"}]},{"given":"Michael","family":"Givskov","sequence":"additional","affiliation":[{"name":"Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark, and Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore"}]},{"given":"Robert P.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee, United Kingdom"}]},{"given":"F\u00e1bio","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica F\u00edsica Molecular and Institute of Nanosciences and Nanotechnologies, Instituto Superior T\u00e9cnico, Lisbon, Portugal"}]},{"given":"Leonilde M.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, Lisbon, Portugal"},{"name":"Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Lisbon, 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