{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T07:10:15Z","timestamp":1768633815755,"version":"3.49.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have constructed a set of plasmids that allow expression, from their native chromosomal loci, of\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            proteins fused to one of four different fluorescent proteins (green fluorescent protein [GFP], cyan fluorescent protein [CFP], yellow fluorescent protein [YFP], and mCherry), using two different resistance markers (kanamycin and erythromycin). We have also constructed a plasmid that allows expression of proteins from the ectopic\n            <jats:italic>spa<\/jats:italic>\n            locus in the\n            <jats:italic>S. aureus<\/jats:italic>\n            chromosome. This toolbox can be used for studies of the localization of proteins in\n            <jats:italic>S. aureus<\/jats:italic>\n            , a prominent pathogen in both health care and community settings.\n          <\/jats:p>","DOI":"10.1128\/aem.00359-10","type":"journal-article","created":{"date-parts":[[2010,5,8]],"date-time":"2010-05-08T02:25:30Z","timestamp":1273285530000},"page":"4346-4353","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Fluorescent Reporters for Studies of Cellular Localization of Proteins in\n            <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Pedro M.","family":"Pereira","sequence":"first","affiliation":[{"name":"Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida da Repu\u0301blica, Apartado 127, 2781-901 Oeiras, Portugal"}]},{"given":"Helena","family":"Veiga","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida da Repu\u0301blica, Apartado 127, 2781-901 Oeiras, Portugal"}]},{"given":"Ana M.","family":"Jorge","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida da Repu\u0301blica, Apartado 127, 2781-901 Oeiras, Portugal"}]},{"given":"Mariana G.","family":"Pinho","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida da Repu\u0301blica, Apartado 127, 2781-901 Oeiras, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"36","volume":"11","year":"2004","unstructured":"Abramoff, M. 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